Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration
The production of nanofibrous materials for soft tissue repair that resemble extracellular matrices (ECMs) is challenging. Electrospinning uniquely produces scaffolds resembling the ultrastructure of natural ECMs. Herein, electrospinning was used to fabricate <i>Bombyx mori</i> silk fibr...
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MDPI AG
2022-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/15/3004 |
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| author | Soheila Mohammadzadehmoghadam Catherine F. LeGrand Chee-Wai Wong Beverley F. Kinnear Yu Dong Deirdre R. Coombe |
| author_facet | Soheila Mohammadzadehmoghadam Catherine F. LeGrand Chee-Wai Wong Beverley F. Kinnear Yu Dong Deirdre R. Coombe |
| author_sort | Soheila Mohammadzadehmoghadam |
| collection | DOAJ |
| description | The production of nanofibrous materials for soft tissue repair that resemble extracellular matrices (ECMs) is challenging. Electrospinning uniquely produces scaffolds resembling the ultrastructure of natural ECMs. Herein, electrospinning was used to fabricate <i>Bombyx mori</i> silk fibroin (SF) and SF/halloysite nanotube (HNT) composite scaffolds. Different HNT loadings were examined, but 1 wt% HNTs enhanced scaffold hydrophilicity and water uptake capacity without loss of mechanical strength. The inclusion of 1 wt% HNTs in SF scaffolds also increased the scaffold’s thermal stability without altering the molecular structure of the SF, as revealed by thermogravimetric analyses and Fourier transform infrared spectroscopy (FTIR), respectively. SF/HNT 1 wt% composite scaffolds better supported the viability and spreading of 3T3 fibroblasts and the differentiation of C2C12 myoblasts into aligned myotubes. These scaffolds coated with decellularised ECM from 3T3 cells or primary human dermal fibroblasts (HDFs) supported the growth of primary human keratinocytes. However, SF/HNT 1 wt% composite scaffolds with HDF-derived ECM provided the best microenvironment, as on these, keratinocytes formed intact monolayers with an undifferentiated, basal cell phenotype. Our data indicate the merits of SF/HNT 1 wt% composite scaffolds for applications in soft tissue repair and the expansion of primary human keratinocytes for skin regeneration. |
| first_indexed | 2024-03-09T05:05:07Z |
| format | Article |
| id | doaj.art-77a7df7caa794f06a47704f19e8bc9f7 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T05:05:07Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-77a7df7caa794f06a47704f19e8bc9f72023-12-03T12:56:00ZengMDPI AGPolymers2073-43602022-07-011415300410.3390/polym14153004Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue RegenerationSoheila Mohammadzadehmoghadam0Catherine F. LeGrand1Chee-Wai Wong2Beverley F. Kinnear3Yu Dong4Deirdre R. Coombe5School of Civil and Mechanical Engineering, Curtin University, Bentley, WA 6102, AustraliaCurtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Bentley, WA 6102, AustraliaCurtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Bentley, WA 6102, AustraliaCurtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Bentley, WA 6102, AustraliaSchool of Civil and Mechanical Engineering, Curtin University, Bentley, WA 6102, AustraliaCurtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Bentley, WA 6102, AustraliaThe production of nanofibrous materials for soft tissue repair that resemble extracellular matrices (ECMs) is challenging. Electrospinning uniquely produces scaffolds resembling the ultrastructure of natural ECMs. Herein, electrospinning was used to fabricate <i>Bombyx mori</i> silk fibroin (SF) and SF/halloysite nanotube (HNT) composite scaffolds. Different HNT loadings were examined, but 1 wt% HNTs enhanced scaffold hydrophilicity and water uptake capacity without loss of mechanical strength. The inclusion of 1 wt% HNTs in SF scaffolds also increased the scaffold’s thermal stability without altering the molecular structure of the SF, as revealed by thermogravimetric analyses and Fourier transform infrared spectroscopy (FTIR), respectively. SF/HNT 1 wt% composite scaffolds better supported the viability and spreading of 3T3 fibroblasts and the differentiation of C2C12 myoblasts into aligned myotubes. These scaffolds coated with decellularised ECM from 3T3 cells or primary human dermal fibroblasts (HDFs) supported the growth of primary human keratinocytes. However, SF/HNT 1 wt% composite scaffolds with HDF-derived ECM provided the best microenvironment, as on these, keratinocytes formed intact monolayers with an undifferentiated, basal cell phenotype. Our data indicate the merits of SF/HNT 1 wt% composite scaffolds for applications in soft tissue repair and the expansion of primary human keratinocytes for skin regeneration.https://www.mdpi.com/2073-4360/14/15/3004silk fibroinelectrospinninghalloysite nanotubesextracellular matrixkeratinocytemyoblast |
| spellingShingle | Soheila Mohammadzadehmoghadam Catherine F. LeGrand Chee-Wai Wong Beverley F. Kinnear Yu Dong Deirdre R. Coombe Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration Polymers silk fibroin electrospinning halloysite nanotubes extracellular matrix keratinocyte myoblast |
| title | Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration |
| title_full | Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration |
| title_fullStr | Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration |
| title_full_unstemmed | Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration |
| title_short | Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration |
| title_sort | fabrication and evaluation of electrospun silk fibroin halloysite nanotube biomaterials for soft tissue regeneration |
| topic | silk fibroin electrospinning halloysite nanotubes extracellular matrix keratinocyte myoblast |
| url | https://www.mdpi.com/2073-4360/14/15/3004 |
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