Effect of Antioxidants (β-mercaptoethanol and Cysteamine) on Assisted Reproductive Technology In vitro
Introduction: Oocyte Culture of Germinal Vesicle (GV) and its growth improves Assisted Reproductive Technology (ART) invitro and infertility. Inappropriate culture medium environment, low quality of oocytes, increase in Oxidative Stress (OS) events, Reactive Oxygen Species (ROS) and free radical...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9298/21778_CE[Ra1]_F(RK)_PF1(SRI_RK)_PFA(AK)_PF2(PRB)_PF3(AG_OM).pdf |
| Summary: | Introduction: Oocyte Culture of Germinal Vesicle (GV) and its
growth improves Assisted Reproductive Technology (ART) invitro
and infertility. Inappropriate culture medium environment, low
quality of oocytes, increase in Oxidative Stress (OS) events,
Reactive Oxygen Species (ROS) and free radicals production are
the main factors that result in unsuccessful Invitro Maturation
(IVM) and decrease in reproduction.
Aim: The present study was conducted with the aim to evaluate
the effect of β-mercaptoethanol (BME) and Cysteamine (CYS)
on IVM improvement, embryo fertilization and development of
blastocyst of mouse immature oocyte.
Materials and Methods: Oocytes were obtained from 4-6
weeks old Naval Medical Research Institute (NMRI) female mice,
48 hours after stimulation with Intraperitoneal (IP) injection of
10 IU Pregnant Mare Serum Gonadotropin (PMSG). GV oocyte
with and without cumulus cells were isolated from ovaries and
cultured in Tissue Culture Medium (TCM) 199 with availability of
100 µM of antioxidants (BME and CYS). After 24 hours, mature
oocyte in metaphase II (MII) were fertilized with sperm in In vitro
Fertilization (IVF) medium (T6) and evaluated for fetal development
into blastocyst.
Results: BME and CYS could significantly (p<0.05) increase
the rate of IVM and oocyte evolution, and embryo formation in
medium culture. Furthermore, it is demonstrated that existence
of Cumulus Oocyte Complexes (COC) significantly showed
better IVM, fertilization and evolution trend as compared to
oocytes without cumulus cover or Denuded Oocytes (DO),
especially in TCM199 plus BME and CYS. So that the change
in GV stage oocytes to MII (maturation rate), fertilization rates
or 2PN formation, and two cell embryos formation or blastocyst
development rate in the treatment group with addition of BME &
CYS and COC was statistically significant as compared to the
DO group (p-value < 0.0001).
Conclusion: Both cellular and environmental factors could be
important and involved in ART improvement. |
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| ISSN: | 2249-782X 0973-709X |