Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates
Developing new approaches for vascularizing synthetic tissue systems will have a tremendous impact in diverse areas. One area where this is particularly important is developing new skeletal muscle tissue systems, which could be utilized in physiological model studies and tissue regeneration. To deve...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/10/907 |
| _version_ | 1797552152552407040 |
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| author | Li Wan James Flegle Burak Ozdoganlar Philip R. LeDuc |
| author_facet | Li Wan James Flegle Burak Ozdoganlar Philip R. LeDuc |
| author_sort | Li Wan |
| collection | DOAJ |
| description | Developing new approaches for vascularizing synthetic tissue systems will have a tremendous impact in diverse areas. One area where this is particularly important is developing new skeletal muscle tissue systems, which could be utilized in physiological model studies and tissue regeneration. To develop vascularized approaches a microfluidic on-chip design for creating channels in polymer systems can be pursued. Current microfluidic tissue engineering methods include soft lithography, rapid prototyping, and cell printing; however, these have limitations such as having their scaffolding being inorganic, less desirable planar vasculature geometry, low fabrication efficiency, and limited resolution. Here we successfully developed a circular microfluidic channel embedded in a 3D extracellular matrix scaffolding with 3D myogenesis. We used a thermo-responsive polymer approach with micromilling-molding and designed a mixture of polyester wax and paraffin wax to fabricate the sacrificial template for microfluidic channel generation in the scaffolding. These findings will impact a number of fields including biomaterials, biomimetic structures, and personalized medicine in the future. |
| first_indexed | 2024-03-10T15:56:53Z |
| format | Article |
| id | doaj.art-77af817b911b40e28867b43567c0de36 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T15:56:53Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-77af817b911b40e28867b43567c0de362023-11-20T15:37:29ZengMDPI AGMicromachines2072-666X2020-09-01111090710.3390/mi11100907Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial TemplatesLi Wan0James Flegle1Burak Ozdoganlar2Philip R. LeDuc3Department of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA 15213, USADepartment of Microbiology, University of Chicago, 5801 S Ellis Ave, Chicago, IL 60637, USADepartment of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA 15213, USADepartment of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA 15213, USADeveloping new approaches for vascularizing synthetic tissue systems will have a tremendous impact in diverse areas. One area where this is particularly important is developing new skeletal muscle tissue systems, which could be utilized in physiological model studies and tissue regeneration. To develop vascularized approaches a microfluidic on-chip design for creating channels in polymer systems can be pursued. Current microfluidic tissue engineering methods include soft lithography, rapid prototyping, and cell printing; however, these have limitations such as having their scaffolding being inorganic, less desirable planar vasculature geometry, low fabrication efficiency, and limited resolution. Here we successfully developed a circular microfluidic channel embedded in a 3D extracellular matrix scaffolding with 3D myogenesis. We used a thermo-responsive polymer approach with micromilling-molding and designed a mixture of polyester wax and paraffin wax to fabricate the sacrificial template for microfluidic channel generation in the scaffolding. These findings will impact a number of fields including biomaterials, biomimetic structures, and personalized medicine in the future.https://www.mdpi.com/2072-666X/11/10/907microfluidicsmuscle-on-a-chipsacrificial template |
| spellingShingle | Li Wan James Flegle Burak Ozdoganlar Philip R. LeDuc Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates Micromachines microfluidics muscle-on-a-chip sacrificial template |
| title | Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates |
| title_full | Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates |
| title_fullStr | Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates |
| title_full_unstemmed | Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates |
| title_short | Toward Vasculature in Skeletal Muscle-on-a-Chip through Thermo-Responsive Sacrificial Templates |
| title_sort | toward vasculature in skeletal muscle on a chip through thermo responsive sacrificial templates |
| topic | microfluidics muscle-on-a-chip sacrificial template |
| url | https://www.mdpi.com/2072-666X/11/10/907 |
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