Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice
Background: Lutein, a carotenoid compound, has previously been studied for its antioxidant and medicinal properties as well as the moderate protection it confers against gamma radiation. This study aimed at evaluating the effects of lutein against radiation-induced hematological and biochemical chan...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2018-01-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/3178 |
| _version_ | 1818276496053108736 |
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| author | Vidya Vasudeva Yogish Somayaji Tenkanidiyoor Alex John Peter Jayaram Shetty Srikant Patil Lakshman Ronald Fernandes Krishna Ananthapura Patali |
| author_facet | Vidya Vasudeva Yogish Somayaji Tenkanidiyoor Alex John Peter Jayaram Shetty Srikant Patil Lakshman Ronald Fernandes Krishna Ananthapura Patali |
| author_sort | Vidya Vasudeva |
| collection | DOAJ |
| description | Background: Lutein, a carotenoid compound, has previously been studied for its antioxidant and medicinal properties as well as the moderate protection it confers against gamma radiation. This study aimed at evaluating the effects of lutein against radiation-induced hematological and biochemical changes in mice.
Methods: The optimized dose of the compound was orally administered for 15 days, and the mice were irradiated (6 Gy) on day 15 after the administration of the compound. The groups were divided (6 mice in each group) into normal control, radiation control, gallic acid control, 10% DMSO control, lutein control, and irradiated groups pretreated with gallic acid, 10% DMSO, and lutein. Gallic acid was used to maintain a standard since it is a proven radioprotector. Within 24 hours post irradiation, the animals were anesthetized and sacrificed. The hematological, biochemical, and antioxidant changes were determined using suitable methods. Data were analyzed by the Kaplan–Meier curve (log-rank test) and ANOVA (the Tukey test). The independent t test was used to compare the independent groups. SPSS (ver. 16) was employed. Results: Maximum survival was observed with a dose of 250 mg/kg b.wt lutein. The total leukocyte count and the percentage lymphocyte count exhibited a significant decline in the irradiated groups pretreated with gallic acid and lutein in comparison to their controls, whereas the percentage granulocyte count showed a significant rise. Antioxidant activity had markedly declined in the irradiated groups, indicating oxidative stress. Lutein pretreatment reduced the damage and maintained the antioxidant system.
Conclusion: The present study suggests a protective role for lutein in palliating radiation-induced oxidative changes and maintaining the antioxidant system in vivo. |
| first_indexed | 2024-12-12T22:46:34Z |
| format | Article |
| id | doaj.art-77b32e5a2ab549f1983e14b834cbe85c |
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| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-12T22:46:34Z |
| publishDate | 2018-01-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-77b32e5a2ab549f1983e14b834cbe85c2022-12-22T00:09:11ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882018-01-014314151Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino MiceVidya Vasudeva0Yogish Somayaji Tenkanidiyoor1Alex John Peter2Jayaram Shetty3Srikant Patil Lakshman4Ronald Fernandes5Krishna Ananthapura Patali6Central Research Laboratory(CRL), K S Hegde Medical Academy, Nitte University, Mangalore, KarnatakaCentral Research Laboratory(CRL), K S Hegde Medical Academy, Nitte University, Mangalore, KarnatakaDepartment of Oncology, Nitte Leela Narayana Shetty Memorial Cancer Institute, Mangalore, KarnatakaDepartment of Oncology, Nitte Leela Narayana Shetty Memorial Cancer Institute, Mangalore, KarnatakaDepartment of Physiology, K S Hegde Medical Academy, Nitte University, Mangalore, KarnatakaDepartment of Biochemistry, Nitte Gulabi Shetty Memorial Institute of Pharmaceutical Sciences, Nitte University, Mangalore, KarnatakaDepartment of Physiology, K S Hegde Medical Academy, Nitte University, Mangalore, KarnatakaBackground: Lutein, a carotenoid compound, has previously been studied for its antioxidant and medicinal properties as well as the moderate protection it confers against gamma radiation. This study aimed at evaluating the effects of lutein against radiation-induced hematological and biochemical changes in mice. Methods: The optimized dose of the compound was orally administered for 15 days, and the mice were irradiated (6 Gy) on day 15 after the administration of the compound. The groups were divided (6 mice in each group) into normal control, radiation control, gallic acid control, 10% DMSO control, lutein control, and irradiated groups pretreated with gallic acid, 10% DMSO, and lutein. Gallic acid was used to maintain a standard since it is a proven radioprotector. Within 24 hours post irradiation, the animals were anesthetized and sacrificed. The hematological, biochemical, and antioxidant changes were determined using suitable methods. Data were analyzed by the Kaplan–Meier curve (log-rank test) and ANOVA (the Tukey test). The independent t test was used to compare the independent groups. SPSS (ver. 16) was employed. Results: Maximum survival was observed with a dose of 250 mg/kg b.wt lutein. The total leukocyte count and the percentage lymphocyte count exhibited a significant decline in the irradiated groups pretreated with gallic acid and lutein in comparison to their controls, whereas the percentage granulocyte count showed a significant rise. Antioxidant activity had markedly declined in the irradiated groups, indicating oxidative stress. Lutein pretreatment reduced the damage and maintained the antioxidant system. Conclusion: The present study suggests a protective role for lutein in palliating radiation-induced oxidative changes and maintaining the antioxidant system in vivo.http://ijms.sums.ac.ir/index.php/IJMS/article/view/3178AntioxidantsCarotenoidsLuteinOxidative stress |
| spellingShingle | Vidya Vasudeva Yogish Somayaji Tenkanidiyoor Alex John Peter Jayaram Shetty Srikant Patil Lakshman Ronald Fernandes Krishna Ananthapura Patali Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice Iranian Journal of Medical Sciences Antioxidants Carotenoids Lutein Oxidative stress |
| title | Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice |
| title_full | Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice |
| title_fullStr | Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice |
| title_full_unstemmed | Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice |
| title_short | Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice |
| title_sort | radioprotective efficacy of lutein in ameliorating electron beam radiation induced oxidative injury in swiss albino mice |
| topic | Antioxidants Carotenoids Lutein Oxidative stress |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/3178 |
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