Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome
Post-intensive care syndrome (PICS) is a physical, cognitive, and mental impairment observed in intensive care unit (ICU) survivors. Although this is an emerging problem in the ICU, how sepsis induces the characteristic symptoms of PICS remains unclear. To develop a model of PICS, we induced sepsis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/8/1593 |
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| author | Yoshihisa Fujinami Shigeaki Inoue Yuko Ono Yusuke Miyazaki Kazumichi Fujioka Kimihiro Yamashita Joji Kotani |
| author_facet | Yoshihisa Fujinami Shigeaki Inoue Yuko Ono Yusuke Miyazaki Kazumichi Fujioka Kimihiro Yamashita Joji Kotani |
| author_sort | Yoshihisa Fujinami |
| collection | DOAJ |
| description | Post-intensive care syndrome (PICS) is a physical, cognitive, and mental impairment observed in intensive care unit (ICU) survivors. Although this is an emerging problem in the ICU, how sepsis induces the characteristic symptoms of PICS remains unclear. To develop a model of PICS, we induced sepsis in male C57/B6 mice via sublethal cecum slurry injection and subsequently treated them using ICU-like interventions. At 1–2 weeks post-sepsis induction, we simultaneously evaluated the abilities of the surviving mice using the following behavioral tests: (1) a grip strength test (GST) and a treadmill test for physical assessment, (2) a novel object recognition test (NORT) for cognitive assessment, and (3) an open field test (OFT) and a marble burying test (MBT) for mental assessment. The surviving mice showed a range of deficits, including muscle weakness with significantly decreased grip strength in the GST; decreased total mileage during the treadmill test; anxiety and decreased activity, with significantly decreased time in the central area, and increased duration of immobility in the OFT; and an increased number of buried marbles in the MBT. Given these physical and mental impairments in the surviving mice, our model has the potential to elucidate mechanistic insights and to discover therapeutic targets and new interventions for PICS. |
| first_indexed | 2024-03-10T12:28:01Z |
| format | Article |
| id | doaj.art-77b7c39f6d3a4bbe9c74697e5bbb48f9 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T12:28:01Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-77b7c39f6d3a4bbe9c74697e5bbb48f92023-11-21T14:54:22ZengMDPI AGJournal of Clinical Medicine2077-03832021-04-01108159310.3390/jcm10081593Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care SyndromeYoshihisa Fujinami0Shigeaki Inoue1Yuko Ono2Yusuke Miyazaki3Kazumichi Fujioka4Kimihiro Yamashita5Joji Kotani6Department of Disaster and Emergency and Critical Care Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Disaster and Emergency and Critical Care Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Disaster and Emergency and Critical Care Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Disaster and Emergency and Critical Care Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Disaster and Emergency and Critical Care Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanPost-intensive care syndrome (PICS) is a physical, cognitive, and mental impairment observed in intensive care unit (ICU) survivors. Although this is an emerging problem in the ICU, how sepsis induces the characteristic symptoms of PICS remains unclear. To develop a model of PICS, we induced sepsis in male C57/B6 mice via sublethal cecum slurry injection and subsequently treated them using ICU-like interventions. At 1–2 weeks post-sepsis induction, we simultaneously evaluated the abilities of the surviving mice using the following behavioral tests: (1) a grip strength test (GST) and a treadmill test for physical assessment, (2) a novel object recognition test (NORT) for cognitive assessment, and (3) an open field test (OFT) and a marble burying test (MBT) for mental assessment. The surviving mice showed a range of deficits, including muscle weakness with significantly decreased grip strength in the GST; decreased total mileage during the treadmill test; anxiety and decreased activity, with significantly decreased time in the central area, and increased duration of immobility in the OFT; and an increased number of buried marbles in the MBT. Given these physical and mental impairments in the surviving mice, our model has the potential to elucidate mechanistic insights and to discover therapeutic targets and new interventions for PICS.https://www.mdpi.com/2077-0383/10/8/1593post-intensive care syndromephysical impairmentscognitive impairmentmental impairmentsepsissurvivor |
| spellingShingle | Yoshihisa Fujinami Shigeaki Inoue Yuko Ono Yusuke Miyazaki Kazumichi Fujioka Kimihiro Yamashita Joji Kotani Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome Journal of Clinical Medicine post-intensive care syndrome physical impairments cognitive impairment mental impairment sepsis survivor |
| title | Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome |
| title_full | Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome |
| title_fullStr | Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome |
| title_full_unstemmed | Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome |
| title_short | Sepsis Induces Physical and Mental Impairments in a Mouse Model of Post-Intensive Care Syndrome |
| title_sort | sepsis induces physical and mental impairments in a mouse model of post intensive care syndrome |
| topic | post-intensive care syndrome physical impairments cognitive impairment mental impairment sepsis survivor |
| url | https://www.mdpi.com/2077-0383/10/8/1593 |
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