| Summary: | We previously constructed a <i>Saccharomyces cerevisiae</i> carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, a transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that <i>ADY2</i>, <i>HES1</i>, and <i>CUP1</i> showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO<sub>4</sub> in YPD media could increase carotenoid yield 9.2-fold. Reverse engineering of target genes was performed and carotenoid yield could be increased 6.4-fold in YPD media through overexpression of <i>ACE1</i>. The present study revealed for the first time the prominent promotion of carotenoid yield by copper ions in engineered <i>S. cerevisiae</i> and provided a new target <i>ACE1</i> for genetic engineering of <i>S. cerevisiae</i> for the bioproduction of carotenoids.
|
|---|