Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i>
We previously constructed a <i>Saccharomyces cerevisiae</i> carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When usi...
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/233 |
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| author | Buli Su Anzhang Li Ming-Rong Deng Honghui Zhu |
| author_facet | Buli Su Anzhang Li Ming-Rong Deng Honghui Zhu |
| author_sort | Buli Su |
| collection | DOAJ |
| description | We previously constructed a <i>Saccharomyces cerevisiae</i> carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, a transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that <i>ADY2</i>, <i>HES1</i>, and <i>CUP1</i> showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO<sub>4</sub> in YPD media could increase carotenoid yield 9.2-fold. Reverse engineering of target genes was performed and carotenoid yield could be increased 6.4-fold in YPD media through overexpression of <i>ACE1</i>. The present study revealed for the first time the prominent promotion of carotenoid yield by copper ions in engineered <i>S. cerevisiae</i> and provided a new target <i>ACE1</i> for genetic engineering of <i>S. cerevisiae</i> for the bioproduction of carotenoids. |
| first_indexed | 2024-03-09T03:53:32Z |
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| id | doaj.art-77ba6e220fab4b00875a3daef286a79a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:53:32Z |
| publishDate | 2021-01-01 |
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| series | Microorganisms |
| spelling | doaj.art-77ba6e220fab4b00875a3daef286a79a2023-12-03T14:24:01ZengMDPI AGMicroorganisms2076-26072021-01-019223310.3390/microorganisms9020233Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i>Buli Su0Anzhang Li1Ming-Rong Deng2Honghui Zhu3State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaWe previously constructed a <i>Saccharomyces cerevisiae</i> carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, a transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that <i>ADY2</i>, <i>HES1</i>, and <i>CUP1</i> showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO<sub>4</sub> in YPD media could increase carotenoid yield 9.2-fold. Reverse engineering of target genes was performed and carotenoid yield could be increased 6.4-fold in YPD media through overexpression of <i>ACE1</i>. The present study revealed for the first time the prominent promotion of carotenoid yield by copper ions in engineered <i>S. cerevisiae</i> and provided a new target <i>ACE1</i> for genetic engineering of <i>S. cerevisiae</i> for the bioproduction of carotenoids.https://www.mdpi.com/2076-2607/9/2/233<i>Saccharomyces cerevisiae</i>carotenoidscopperzinc<i>ACE1</i><i>ADY2</i> |
| spellingShingle | Buli Su Anzhang Li Ming-Rong Deng Honghui Zhu Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i> Microorganisms <i>Saccharomyces cerevisiae</i> carotenoids copper zinc <i>ACE1</i> <i>ADY2</i> |
| title | Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i> |
| title_full | Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i> |
| title_short | Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant <i>Saccharomyces cerevisiae</i> |
| title_sort | transcriptome analysis reveals a promotion of carotenoid production by copper ions in recombinant i saccharomyces cerevisiae i |
| topic | <i>Saccharomyces cerevisiae</i> carotenoids copper zinc <i>ACE1</i> <i>ADY2</i> |
| url | https://www.mdpi.com/2076-2607/9/2/233 |
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