Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples
Introduction: First spotted in Wuhan, China, World Health Organisation declared the deadly outbreak caused by novel Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2) as global pandemic on March 11, 2020. With over 72 million cases globally till December 2020, countries need to gear u...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2021-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/14795/48127_CE[Ra]_F(Sh)_PF1(AG_SL)_PFA(KM)_PB(AG_KM)_PN(KM).pdf |
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| author | Prashant Patil Pratik Thosani Santosh Karade Kavita Bala Anand SPS Shergill Sourav Sen Rajiv Mohan Gupta |
| author_facet | Prashant Patil Pratik Thosani Santosh Karade Kavita Bala Anand SPS Shergill Sourav Sen Rajiv Mohan Gupta |
| author_sort | Prashant Patil |
| collection | DOAJ |
| description | Introduction: First spotted in Wuhan, China, World Health
Organisation declared the deadly outbreak caused by novel
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS
CoV-2) as global pandemic on March 11, 2020. With over
72 million cases globally till December 2020, countries need to
gear up to detect, isolate and treat Corona Virus Disease-2019
(COVID-19) cases. Laboratories play a crucial role in diagnosis
thereby instituting early contact tracing and quarantine
measures. The laboratory based COVID-19 diagnostic testing
data may help in formulating strategies to contain the spread
of infection.
Aim: To describe key patient variables of the respiratory samples
processed for SARS-CoV-2 by Real Time Polymerase Chain
Reaction (RT-PCR) at a diagnostic laboratory.
Materials and Methods: In this descriptive, single centre
study carried out at ICMR approved COVID-19 diagnostic
laboratory, nasopharyngeal swabs received in Viral Transport
Medium (VTM) were tested for SARS-CoV-2 infection by RTPCR. Key patient variables, like age, gender, symptoms and
sample positivity rate were tabulated. The demographic and
clinical data of samples tested were summarised by medians
and Interquartile Range (IQR) for continuous variables and by
proportions for categorical variables.
Results: A total of 12,187 samples were received between
21st March to 8th July 2020. The data from 11,196 individuals
were complete and were included in the analysis. Overall,
2,053 samples were tested positive for SARS-CoV-2 indicating
positivity rate of 18.33%. Sample positivity was highest (63.91%)
among high-risk contacts of a laboratory confirmed case. The
maximum number of samples tested belonged to age group of
21-40 years and male predominance was observed.
Conclusion: Although social distancing, mask usage, hand
hygiene and respiratory etiquettes are important measures for
containment of COVID-19, strengthening and capacity building
of laboratory network is crucial for mitigating the pandemic. |
| first_indexed | 2024-12-14T17:43:29Z |
| format | Article |
| id | doaj.art-77beb689b490439e9675a870c12bbe31 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T17:43:29Z |
| publishDate | 2021-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-77beb689b490439e9675a870c12bbe312022-12-21T22:52:47ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-04-01154DC09DC1210.7860/JCDR/2021/48127.14795Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 SamplesPrashant Patil0Pratik Thosani1Santosh Karade2Kavita Bala Anand3SPS Shergill4Sourav Sen5Rajiv Mohan Gupta6Resident, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India.Resident, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India.Associate Professor, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India.Associate Professor, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India.Associate Professor, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India.Professor and Head, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India.Professor and Dean, Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India. PLAGIARISM CHECKIntroduction: First spotted in Wuhan, China, World Health Organisation declared the deadly outbreak caused by novel Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2) as global pandemic on March 11, 2020. With over 72 million cases globally till December 2020, countries need to gear up to detect, isolate and treat Corona Virus Disease-2019 (COVID-19) cases. Laboratories play a crucial role in diagnosis thereby instituting early contact tracing and quarantine measures. The laboratory based COVID-19 diagnostic testing data may help in formulating strategies to contain the spread of infection. Aim: To describe key patient variables of the respiratory samples processed for SARS-CoV-2 by Real Time Polymerase Chain Reaction (RT-PCR) at a diagnostic laboratory. Materials and Methods: In this descriptive, single centre study carried out at ICMR approved COVID-19 diagnostic laboratory, nasopharyngeal swabs received in Viral Transport Medium (VTM) were tested for SARS-CoV-2 infection by RTPCR. Key patient variables, like age, gender, symptoms and sample positivity rate were tabulated. The demographic and clinical data of samples tested were summarised by medians and Interquartile Range (IQR) for continuous variables and by proportions for categorical variables. Results: A total of 12,187 samples were received between 21st March to 8th July 2020. The data from 11,196 individuals were complete and were included in the analysis. Overall, 2,053 samples were tested positive for SARS-CoV-2 indicating positivity rate of 18.33%. Sample positivity was highest (63.91%) among high-risk contacts of a laboratory confirmed case. The maximum number of samples tested belonged to age group of 21-40 years and male predominance was observed. Conclusion: Although social distancing, mask usage, hand hygiene and respiratory etiquettes are important measures for containment of COVID-19, strengthening and capacity building of laboratory network is crucial for mitigating the pandemic.https://www.jcdr.net/articles/PDF/14795/48127_CE[Ra]_F(Sh)_PF1(AG_SL)_PFA(KM)_PB(AG_KM)_PN(KM).pdfreal time polymerase chain reactionrespiratory infectionsevere acute respiratory syndrome coronavirus-2 |
| spellingShingle | Prashant Patil Pratik Thosani Santosh Karade Kavita Bala Anand SPS Shergill Sourav Sen Rajiv Mohan Gupta Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples Journal of Clinical and Diagnostic Research real time polymerase chain reaction respiratory infection severe acute respiratory syndrome coronavirus-2 |
| title | Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples |
| title_full | Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples |
| title_fullStr | Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples |
| title_full_unstemmed | Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples |
| title_short | Laboratory Diagnosis of SARS Corona Virus-2 Infection: Single Centre Experience of First 12,000 Samples |
| title_sort | laboratory diagnosis of sars corona virus 2 infection single centre experience of first 12 000 samples |
| topic | real time polymerase chain reaction respiratory infection severe acute respiratory syndrome coronavirus-2 |
| url | https://www.jcdr.net/articles/PDF/14795/48127_CE[Ra]_F(Sh)_PF1(AG_SL)_PFA(KM)_PB(AG_KM)_PN(KM).pdf |
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