Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique
This study carried out to direct molecular investigation of Entamoeba bovis from feces samples of calves which suffering from diarrhea that collected from different fields in Al-Diwanyia city by using polymerase chain reaction technique (PCR). This technique was dependent on used specific primers...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, University of Kufa
2016-06-01
|
| Series: | Kufa Journal for Veterinary Medical Sciences |
| Subjects: | |
| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4278 |
| _version_ | 1827389078093430784 |
|---|---|
| author | Amal Hassan Al-shabbani |
| author_facet | Amal Hassan Al-shabbani |
| author_sort | Amal Hassan Al-shabbani |
| collection | DOAJ |
| description |
This study carried out to direct molecular investigation of Entamoeba bovis from feces samples of calves which suffering from diarrhea that collected from different fields in Al-Diwanyia city by using polymerase chain reaction technique (PCR). This technique was dependent on used specific primers that amplification of small subunit ribosomal RNA gene in Entamoeba bovis. This primers were designed in this study by using NCBI-Genk data base (FN666248.1) and primer 3 plus for primers design. The PCR results were appeared that cattle infected with Entamoeba bovis in percentage of about (36% ) 18 positive samples out of 50 diarrheic samples. We concluded that Entamoeba bovis is important causes of enteric infection in calf whereas, the polymerase chain reaction technique is very specific and rapid assay.
|
| first_indexed | 2024-03-08T16:29:22Z |
| format | Article |
| id | doaj.art-77c15b5f7c034758ba74988165aa0f67 |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:22Z |
| publishDate | 2016-06-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-77c15b5f7c034758ba74988165aa0f672024-01-06T10:21:42ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782016-06-017110.36326/kjvs/2016/v7i14278Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction techniqueAmal Hassan Al-shabbani This study carried out to direct molecular investigation of Entamoeba bovis from feces samples of calves which suffering from diarrhea that collected from different fields in Al-Diwanyia city by using polymerase chain reaction technique (PCR). This technique was dependent on used specific primers that amplification of small subunit ribosomal RNA gene in Entamoeba bovis. This primers were designed in this study by using NCBI-Genk data base (FN666248.1) and primer 3 plus for primers design. The PCR results were appeared that cattle infected with Entamoeba bovis in percentage of about (36% ) 18 positive samples out of 50 diarrheic samples. We concluded that Entamoeba bovis is important causes of enteric infection in calf whereas, the polymerase chain reaction technique is very specific and rapid assay. https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4278Calvesentamoeba bovispolymerase chain reaction |
| spellingShingle | Amal Hassan Al-shabbani Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique Kufa Journal for Veterinary Medical Sciences Calves entamoeba bovis polymerase chain reaction |
| title | Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique |
| title_full | Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique |
| title_fullStr | Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique |
| title_full_unstemmed | Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique |
| title_short | Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique |
| title_sort | direct detection of entamoeba bovis in calves infected by diarrhea by using polymerase chain reaction technique |
| topic | Calves entamoeba bovis polymerase chain reaction |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4278 |
| work_keys_str_mv | AT amalhassanalshabbani directdetectionofentamoebabovisincalvesinfectedbydiarrheabyusingpolymerasechainreactiontechnique |