Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study
Ciliary neurotrophic factor (CNTF) is a member of interleukin-6 type cytokine family. The CNTF receptor complex is a heterodimer including gp130 and CNTF receptor α (CNTFRα) proteins triggering the activation of multiple intracellular signaling pathways including AKT/PI3K, MAPK/ERK and Jak/STAT path...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2020-10-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3147 |
| _version_ | 1828490511329001472 |
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| author | Sonia Fantone Giovanni Tossetta Rodolfo Montironi Martina Senzacqua Daniela Marzioni Roberta Mazzucchelli |
| author_facet | Sonia Fantone Giovanni Tossetta Rodolfo Montironi Martina Senzacqua Daniela Marzioni Roberta Mazzucchelli |
| author_sort | Sonia Fantone |
| collection | DOAJ |
| description | Ciliary neurotrophic factor (CNTF) is a member of interleukin-6 type cytokine family. The CNTF receptor complex is a heterodimer including gp130 and CNTF receptor α (CNTFRα) proteins triggering the activation of multiple intracellular signaling pathways including AKT/PI3K, MAPK/ERK and Jak/STAT pathways. At present no data are available on the localization of CNTF and CNTFRα in prostate as well as on the role of CNTF in this organ. In this study we have analyzed the localization of CNTF and CNTFRα by immunohistochemistry and we have used PWR-1E cell line as a model for normal glandular cell to investigate the role of this cytokine. Our results show that CNTF and CNTFRa are expressed in the staminal compart of the prostate and that CNTF selectively inhibits ERK pathway. In conclusion, we suggest that CNTF could be considered as key molecule to maintenance epithelium homeostasis via pERK downregulation by an autocrine mechanism. Further CNTF studies in prostate cancer could be useful to verify the potential role of this cytokine in carcinogenesis. |
| first_indexed | 2024-12-11T10:46:08Z |
| format | Article |
| id | doaj.art-77c3402ac53d436c8d76328482c6d001 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T10:46:08Z |
| publishDate | 2020-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-77c3402ac53d436c8d76328482c6d0012022-12-22T01:10:28ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062020-10-0164410.4081/ejh.2020.3147Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular studySonia Fantone0Giovanni Tossetta1Rodolfo Montironi2Martina Senzacqua 3Daniela Marzioni4Roberta Mazzucchelli5Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, AnconaDepartment of Experimental and Clinical Medicine, Università Politecnica delle Marche, AnconaDepartment of Biomedical Sciences and Public Health, Section of Pathological Anatomy, Università Politecnica delle Marche, School of Medicine, United Hospitals, AnconaDepartment of Experimental and Clinical Medicine, Università Politecnica delle Marche, AnconaDepartment of Experimental and Clinical Medicine, Università Politecnica delle Marche, AnconaDepartment of Biomedical Sciences and Public Health, Section of Pathological Anatomy, Università Politecnica delle Marche, School of Medicine, United Hospitals, AnconaCiliary neurotrophic factor (CNTF) is a member of interleukin-6 type cytokine family. The CNTF receptor complex is a heterodimer including gp130 and CNTF receptor α (CNTFRα) proteins triggering the activation of multiple intracellular signaling pathways including AKT/PI3K, MAPK/ERK and Jak/STAT pathways. At present no data are available on the localization of CNTF and CNTFRα in prostate as well as on the role of CNTF in this organ. In this study we have analyzed the localization of CNTF and CNTFRα by immunohistochemistry and we have used PWR-1E cell line as a model for normal glandular cell to investigate the role of this cytokine. Our results show that CNTF and CNTFRa are expressed in the staminal compart of the prostate and that CNTF selectively inhibits ERK pathway. In conclusion, we suggest that CNTF could be considered as key molecule to maintenance epithelium homeostasis via pERK downregulation by an autocrine mechanism. Further CNTF studies in prostate cancer could be useful to verify the potential role of this cytokine in carcinogenesis.https://www.ejh.it/index.php/ejh/article/view/3147PWR-1E cell lineimmunohistochemistryWestern blottingcytokine |
| spellingShingle | Sonia Fantone Giovanni Tossetta Rodolfo Montironi Martina Senzacqua Daniela Marzioni Roberta Mazzucchelli Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study European Journal of Histochemistry PWR-1E cell line immunohistochemistry Western blotting cytokine |
| title | Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study |
| title_full | Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study |
| title_fullStr | Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study |
| title_full_unstemmed | Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study |
| title_short | Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study |
| title_sort | ciliary neurotrophic factor cntf and its receptor cntfrα signal through mapk erk pathway in human prostate tissues a morphological and biomolecular study |
| topic | PWR-1E cell line immunohistochemistry Western blotting cytokine |
| url | https://www.ejh.it/index.php/ejh/article/view/3147 |
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