Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i>
Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/11/595 |
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| author | Yanan Xu Patricia J. Harvey |
| author_facet | Yanan Xu Patricia J. Harvey |
| author_sort | Yanan Xu |
| collection | DOAJ |
| description | Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga <i>Dunaliella salina</i> is one of the richest sources of natural carotenoids. We have previously investigated the over-production of phytoene in <i>D. salina</i> after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in <i>D. salina</i>. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support the over-production of phytoene by <i>D. salina</i> cultures as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h of treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in <i>D. salina</i>. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from <i>D. salina</i>. |
| first_indexed | 2024-03-10T05:19:45Z |
| format | Article |
| id | doaj.art-77c42300c06748b88b083a7c7c54ce2e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T05:19:45Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-77c42300c06748b88b083a7c7c54ce2e2023-11-23T00:07:00ZengMDPI AGMarine Drugs1660-33972021-10-01191159510.3390/md19110595Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i>Yanan Xu0Patricia J. Harvey1Faculty of Engineering and Science, University of Greenwich, Central Avenue, Chatham Maritime, Kent ME4 4TB, UKFaculty of Engineering and Science, University of Greenwich, Central Avenue, Chatham Maritime, Kent ME4 4TB, UKPhytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga <i>Dunaliella salina</i> is one of the richest sources of natural carotenoids. We have previously investigated the over-production of phytoene in <i>D. salina</i> after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in <i>D. salina</i>. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support the over-production of phytoene by <i>D. salina</i> cultures as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h of treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in <i>D. salina</i>. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from <i>D. salina</i>.https://www.mdpi.com/1660-3397/19/11/595phytoene<i>Dunaliella salina</i>mitosis inhibitorsherbicidesred light |
| spellingShingle | Yanan Xu Patricia J. Harvey Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i> Marine Drugs phytoene <i>Dunaliella salina</i> mitosis inhibitors herbicides red light |
| title | Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i> |
| title_full | Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i> |
| title_fullStr | Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i> |
| title_full_unstemmed | Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i> |
| title_short | Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga <i>Dunaliella salina</i> |
| title_sort | mitosis inhibitors induce massive accumulation of phytoene in the microalga i dunaliella salina i |
| topic | phytoene <i>Dunaliella salina</i> mitosis inhibitors herbicides red light |
| url | https://www.mdpi.com/1660-3397/19/11/595 |
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