Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101
The main objective of this study was to standardise the procedure for encapsulation of potentially probiotic breast milk isolate Pediococcus pentosaceus DM101 using sodium alginate by extrusion method. The encapsulation parameters were optimized by comparing the encapsulation efficiency obtained wi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2021-06-01
|
| Series: | Journal of Veterinary and Animal Sciences |
| Subjects: | |
| Online Access: | https://www.jvas.in/public_html/upload/article_file/article_file_qvr9f3.pdf?t=qvr9f4 |
| _version_ | 1818439236224811008 |
|---|---|
| author | Jithu Rappai A.K. Beena Ligimol James V. Aparna Sudhakaran |
| author_facet | Jithu Rappai A.K. Beena Ligimol James V. Aparna Sudhakaran |
| author_sort | Jithu Rappai |
| collection | DOAJ |
| description | The main objective of this study was to standardise the procedure for encapsulation of
potentially probiotic breast milk isolate Pediococcus pentosaceus DM101 using sodium alginate by extrusion method. The encapsulation parameters were optimized by comparing the encapsulation efficiency obtained with varying concentration of sodium alginate (0.5, 1.0, 1.5 and 2.0%), calcium chloride (0.05, 0.1 and 0.2M) and gelling time (5, 10, 20 and 30 minutes). Encapsulation efficiency was ascertained by finding the survival percentage after exposing the encapsulated cells to acid stress (pH 2.0 for 3h). Encapsulation carried out using two per cent sodium alginate and 0.1M
CaCl2 following 20 min gelling time was found to confer maximum protective effect. Encapsulation efficiency of alginate beads prepared under optimized conditions was found to be 81.37±2.44 percent. The results endorse alginate encapsulation as a means to confer a protective shielding effect thereby facilitating effective probiotic delivery. |
| first_indexed | 2024-12-14T17:53:15Z |
| format | Article |
| id | doaj.art-77c560450c44478aa042ca8749694de0 |
| institution | Directory Open Access Journal |
| issn | 0971-0701 2582-0605 |
| language | English |
| last_indexed | 2024-12-14T17:53:15Z |
| publishDate | 2021-06-01 |
| publisher | Director of Academics and Research, Kerala Veterinary and Animal Sciences University |
| record_format | Article |
| series | Journal of Veterinary and Animal Sciences |
| spelling | doaj.art-77c560450c44478aa042ca8749694de02022-12-21T22:52:36ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052021-06-0152219619910.51966/jvas.2021.52.2.196-199Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101Jithu Rappai0 A.K. Beena1https://orcid.org/0000-0001-7263-5265Ligimol James2V. Aparna Sudhakaran3M. Tech scholarDepartment of Dairy Microbiology College of Dairy Science and Technology, Mannuthy, Thrissur, Kerala-680651, India. Kerala Veterinary and Animal Sciences UniversityDepartment of Dairy Microbiology College of Dairy Science and Technology, Mannuthy, Thrissur, Kerala-680651, India. Kerala Veterinary and Animal Sciences UniversityDepartment of Dairy Microbiology College of Dairy Science and Technology, Mannuthy, Thrissur, Kerala-680651, India. Kerala Veterinary and Animal Sciences UniversityThe main objective of this study was to standardise the procedure for encapsulation of potentially probiotic breast milk isolate Pediococcus pentosaceus DM101 using sodium alginate by extrusion method. The encapsulation parameters were optimized by comparing the encapsulation efficiency obtained with varying concentration of sodium alginate (0.5, 1.0, 1.5 and 2.0%), calcium chloride (0.05, 0.1 and 0.2M) and gelling time (5, 10, 20 and 30 minutes). Encapsulation efficiency was ascertained by finding the survival percentage after exposing the encapsulated cells to acid stress (pH 2.0 for 3h). Encapsulation carried out using two per cent sodium alginate and 0.1M CaCl2 following 20 min gelling time was found to confer maximum protective effect. Encapsulation efficiency of alginate beads prepared under optimized conditions was found to be 81.37±2.44 percent. The results endorse alginate encapsulation as a means to confer a protective shielding effect thereby facilitating effective probiotic delivery.https://www.jvas.in/public_html/upload/article_file/article_file_qvr9f3.pdf?t=qvr9f4probioticoptimizationsodium alginatepediococcus pentosaceus |
| spellingShingle | Jithu Rappai A.K. Beena Ligimol James V. Aparna Sudhakaran Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101 Journal of Veterinary and Animal Sciences probiotic optimization sodium alginate pediococcus pentosaceus |
| title | Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101 |
| title_full | Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101 |
| title_fullStr | Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101 |
| title_full_unstemmed | Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101 |
| title_short | Process standardization for alginate encapsulation of potentially probiotic Pediococcus pentosaceus DM101 |
| title_sort | process standardization for alginate encapsulation of potentially probiotic pediococcus pentosaceus dm101 |
| topic | probiotic optimization sodium alginate pediococcus pentosaceus |
| url | https://www.jvas.in/public_html/upload/article_file/article_file_qvr9f3.pdf?t=qvr9f4 |
| work_keys_str_mv | AT jithurappai processstandardizationforalginateencapsulationofpotentiallyprobioticpediococcuspentosaceusdm101 AT akbeena processstandardizationforalginateencapsulationofpotentiallyprobioticpediococcuspentosaceusdm101 AT ligimoljames processstandardizationforalginateencapsulationofpotentiallyprobioticpediococcuspentosaceusdm101 AT vaparnasudhakaran processstandardizationforalginateencapsulationofpotentiallyprobioticpediococcuspentosaceusdm101 |