Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal
Background & objectives: Epidemiological interventions and mosquito control are the available measures for dengue control. The former approach uses serotype and genetic information on the circulating virus strains. Dengue has been frequently reported from Nepal, but this information is mostly la...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Indian Journal of Medical Research |
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| Online Access: | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=142;issue=7;spage=1;epage=6;aulast=Gupta |
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| author | Birendra Prasad Gupta Sneha Singh Roshan Kurmi Rajani Malla Easwaran Sreekumar Krishna Das Manandhar |
| author_facet | Birendra Prasad Gupta Sneha Singh Roshan Kurmi Rajani Malla Easwaran Sreekumar Krishna Das Manandhar |
| author_sort | Birendra Prasad Gupta |
| collection | DOAJ |
| description | Background & objectives: Epidemiological interventions and mosquito control are the available measures for dengue control. The former approach uses serotype and genetic information on the circulating virus strains. Dengue has been frequently reported from Nepal, but this information is mostly lacking. The present study was done to generate a comprehensive clinical and virological picture of a dengue outbreak in Nepal during 2013.
Methods: A hospital-based study involving patients from five districts of Nepal was carried out. Demographic information, clinical details and dengue serological status were obtained. Viral RNA was characterized at the molecular level by reverse-transcription polymerase chain reaction (RT-PCR), nucleotide sequencing and phylogenetic analysis.
Results: From among the 2340 laboratory-confirmed dengue cases during the study period, 198 patients consented for the study. Clinically they had fever (100%), headache (59.1%), rashes (18.2%), retro-orbital pain (30.3%), vomiting (15.1%), joint pain (28.8%) and thrombocytopenia (74.3%). Fifteen (7.5%) of them had mucosal bleeding manifestations, and the rest were uncomplicated dengue fever. The patients were mostly adults with a mean age of 45.75 ± 38.61 yr. Of the 52 acute serum samples tested, 15 were positive in RT-PCR. The causative virus was identified as DENV serotype 2 belonging to the Cosmopolitan genotype.
Interpretations & conclusions: We report here the involvement of DENV serotype 2 in an outbreak in Nepal in 2013. Earlier outbreaks in the region in 2010 were attributed to serotype 1 virus. As serotype shifts are frequently associated with secondary infections and severe disease, there is a need for enhancing surveillance especially in the monsoon and post-monsoon periods to prevent large-scale, severe dengue outbreaks in the region. |
| first_indexed | 2024-04-11T23:00:51Z |
| format | Article |
| id | doaj.art-77c859826f994bae8448f4cb01054b40 |
| institution | Directory Open Access Journal |
| issn | 0971-5916 |
| language | English |
| last_indexed | 2024-04-11T23:00:51Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Medical Research |
| spelling | doaj.art-77c859826f994bae8448f4cb01054b402022-12-22T03:58:10ZengWolters Kluwer Medknow PublicationsIndian Journal of Medical Research0971-59162015-01-0114271610.4103/0971-5916.176564Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in NepalBirendra Prasad GuptaSneha SinghRoshan KurmiRajani MallaEaswaran SreekumarKrishna Das ManandharBackground & objectives: Epidemiological interventions and mosquito control are the available measures for dengue control. The former approach uses serotype and genetic information on the circulating virus strains. Dengue has been frequently reported from Nepal, but this information is mostly lacking. The present study was done to generate a comprehensive clinical and virological picture of a dengue outbreak in Nepal during 2013. Methods: A hospital-based study involving patients from five districts of Nepal was carried out. Demographic information, clinical details and dengue serological status were obtained. Viral RNA was characterized at the molecular level by reverse-transcription polymerase chain reaction (RT-PCR), nucleotide sequencing and phylogenetic analysis. Results: From among the 2340 laboratory-confirmed dengue cases during the study period, 198 patients consented for the study. Clinically they had fever (100%), headache (59.1%), rashes (18.2%), retro-orbital pain (30.3%), vomiting (15.1%), joint pain (28.8%) and thrombocytopenia (74.3%). Fifteen (7.5%) of them had mucosal bleeding manifestations, and the rest were uncomplicated dengue fever. The patients were mostly adults with a mean age of 45.75 ± 38.61 yr. Of the 52 acute serum samples tested, 15 were positive in RT-PCR. The causative virus was identified as DENV serotype 2 belonging to the Cosmopolitan genotype. Interpretations & conclusions: We report here the involvement of DENV serotype 2 in an outbreak in Nepal in 2013. Earlier outbreaks in the region in 2010 were attributed to serotype 1 virus. As serotype shifts are frequently associated with secondary infections and severe disease, there is a need for enhancing surveillance especially in the monsoon and post-monsoon periods to prevent large-scale, severe dengue outbreaks in the region.http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=142;issue=7;spage=1;epage=6;aulast=GuptaDengue fever - dengue virus type 2 - India - Nepal - phylogeny |
| spellingShingle | Birendra Prasad Gupta Sneha Singh Roshan Kurmi Rajani Malla Easwaran Sreekumar Krishna Das Manandhar Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal Indian Journal of Medical Research Dengue fever - dengue virus type 2 - India - Nepal - phylogeny |
| title | Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal |
| title_full | Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal |
| title_fullStr | Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal |
| title_full_unstemmed | Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal |
| title_short | Re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in Nepal |
| title_sort | re emergence of dengue virus serotype 2 strains in the 2013 outbreak in nepal |
| topic | Dengue fever - dengue virus type 2 - India - Nepal - phylogeny |
| url | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=142;issue=7;spage=1;epage=6;aulast=Gupta |
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