Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada
Abstract An eight-week trial was designed to explore the dietary effects of commercially purchased exogenous bile acids (BAs) on growth, whole-body composition, lipid profile, intestinal digestive enzymes, liver function enzymes, oxidative stress biomarkers, and serum immunity of thinlip mullet, Liz...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49788-6 |
| _version_ | 1797376981073920000 |
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| author | Mohsen Abdel-Tawwab Hany M. R. Abdel-Latif Mohammed F. El Basuini Asmaa M. El-Nokrashy Asmaa A. Khaled Mohamed Kord Ali A. Soliman Mohamed Zaki Abd-Elaziz Nour Eman M. H. Labib Hala Saber Khalil |
| author_facet | Mohsen Abdel-Tawwab Hany M. R. Abdel-Latif Mohammed F. El Basuini Asmaa M. El-Nokrashy Asmaa A. Khaled Mohamed Kord Ali A. Soliman Mohamed Zaki Abd-Elaziz Nour Eman M. H. Labib Hala Saber Khalil |
| author_sort | Mohsen Abdel-Tawwab |
| collection | DOAJ |
| description | Abstract An eight-week trial was designed to explore the dietary effects of commercially purchased exogenous bile acids (BAs) on growth, whole-body composition, lipid profile, intestinal digestive enzymes, liver function enzymes, oxidative stress biomarkers, and serum immunity of thinlip mullet, Liza ramada. Four triplicate groups (10.50 ± 0.05 g) were fed four soybean meal (SBM)-based diets supplied with several BAs levels at 0 (control), 50, 130, or 350 mg/kg feed. Results indicated that the growth was significantly increased in groups fed BAs-based diets, especially at 130 mg/kg feed. The body composition analysis showed that feeding fish on diets supplied with BAs up to 130 mg/kg decreased moisture (%) alongside increased crude protein (%). However, the body composition of fish fed a diet with 350 mg BAs/kg had the lowest moisture (%) and the highest crude protein (%). Moreover, there were significant increases in the intestinal (protease, α-amylase, and lipase) enzyme activities in the groups supplied with BAs up to 130 mg BAs/kg. Liver function enzymes (aspartate aminotransferase and alanine aminotransferase enzyme activities) were significantly decreased in BAs-supplemented groups compared to those fed the BAs-free group. On the other hand, the control group had higher total cholesterol, triglycerides, and low-density lipoprotein alongside the lower high-density lipoprotein than BAs-supplemented groups, especially at 350 mg BAs/kg feed. BAs significantly decreased hepatic malondialdehyde concentrations and increased the activity of hepatic catalase, superoxide dismutase, and total antioxidant capacity compared with those reared on the control diet. Serum lysozyme, respiratory burst, and alternative complement activities were significantly increased in BAs-supplied groups, particularly in the group supplied with 130 mg BAs/kg compared to those fed on the control diet. Accordingly, our findings recommend that including 130 mg BAs/kg in an SBM-based diet enhanced the growth, digestive enzyme activities, and liver functions, alleviated oxidative stress, boosted serum immunity, and lowered lipid metabolites in thinlip mullet. These findings will be beneficial for improving the quality of feed prepared for feeding mullets and an effective alternative strategy to support mullet farming. |
| first_indexed | 2024-03-08T19:46:23Z |
| format | Article |
| id | doaj.art-77cdae26a1f34da994e238b3864ea250 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T19:46:23Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-77cdae26a1f34da994e238b3864ea2502023-12-24T12:19:17ZengNature PortfolioScientific Reports2045-23222023-12-0113111410.1038/s41598-023-49788-6Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramadaMohsen Abdel-Tawwab0Hany M. R. Abdel-Latif1Mohammed F. El Basuini2Asmaa M. El-Nokrashy3Asmaa A. Khaled4Mohamed Kord5Ali A. Soliman6Mohamed Zaki7Abd-Elaziz Nour8Eman M. H. Labib9Hala Saber Khalil10Department of Fish Biology and Ecology, Central Laboratory for Aquaculture Research, Agriculture Research CenterDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria UniversityAnimal Production Department, Faculty of Agriculture, Tanta UniversityDepartment of Aquaculture, Faculty of Aquatic and Fisheries Science, Kafrelsheikh UniversityAnimal and Fish Production Department, Faculty of Agriculture (Saba Basha), Alexandria UniversityCentral Laboratory for Agricultural Climate, ARCNational Institute of Oceanography and Fisheries (NIOF)Animal Production Department, Faculty of Agriculture, Alexandria UniversityAnimal Production Department, Faculty of Agriculture, Alexandria UniversityUtilization of By-Products Department, Animal Production Research Institute, Agriculture Research Center, Ministry of Agriculture and Land ReclamationAquaculture Department, Faculty of Fish Resources, Suez UniversityAbstract An eight-week trial was designed to explore the dietary effects of commercially purchased exogenous bile acids (BAs) on growth, whole-body composition, lipid profile, intestinal digestive enzymes, liver function enzymes, oxidative stress biomarkers, and serum immunity of thinlip mullet, Liza ramada. Four triplicate groups (10.50 ± 0.05 g) were fed four soybean meal (SBM)-based diets supplied with several BAs levels at 0 (control), 50, 130, or 350 mg/kg feed. Results indicated that the growth was significantly increased in groups fed BAs-based diets, especially at 130 mg/kg feed. The body composition analysis showed that feeding fish on diets supplied with BAs up to 130 mg/kg decreased moisture (%) alongside increased crude protein (%). However, the body composition of fish fed a diet with 350 mg BAs/kg had the lowest moisture (%) and the highest crude protein (%). Moreover, there were significant increases in the intestinal (protease, α-amylase, and lipase) enzyme activities in the groups supplied with BAs up to 130 mg BAs/kg. Liver function enzymes (aspartate aminotransferase and alanine aminotransferase enzyme activities) were significantly decreased in BAs-supplemented groups compared to those fed the BAs-free group. On the other hand, the control group had higher total cholesterol, triglycerides, and low-density lipoprotein alongside the lower high-density lipoprotein than BAs-supplemented groups, especially at 350 mg BAs/kg feed. BAs significantly decreased hepatic malondialdehyde concentrations and increased the activity of hepatic catalase, superoxide dismutase, and total antioxidant capacity compared with those reared on the control diet. Serum lysozyme, respiratory burst, and alternative complement activities were significantly increased in BAs-supplied groups, particularly in the group supplied with 130 mg BAs/kg compared to those fed on the control diet. Accordingly, our findings recommend that including 130 mg BAs/kg in an SBM-based diet enhanced the growth, digestive enzyme activities, and liver functions, alleviated oxidative stress, boosted serum immunity, and lowered lipid metabolites in thinlip mullet. These findings will be beneficial for improving the quality of feed prepared for feeding mullets and an effective alternative strategy to support mullet farming.https://doi.org/10.1038/s41598-023-49788-6 |
| spellingShingle | Mohsen Abdel-Tawwab Hany M. R. Abdel-Latif Mohammed F. El Basuini Asmaa M. El-Nokrashy Asmaa A. Khaled Mohamed Kord Ali A. Soliman Mohamed Zaki Abd-Elaziz Nour Eman M. H. Labib Hala Saber Khalil Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada Scientific Reports |
| title | Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada |
| title_full | Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada |
| title_fullStr | Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada |
| title_full_unstemmed | Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada |
| title_short | Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada |
| title_sort | effects of exogenous bile acids bas on growth lipid profile digestive enzymes and immune responses of thinlip mullet liza ramada |
| url | https://doi.org/10.1038/s41598-023-49788-6 |
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