Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability
Background: Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320423001104 |
| _version_ | 1827630305883717632 |
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| author | Ryoko Inaki Yoshihiko Sato Daisuke Nakamura Yoshiyuki Aikawa Tsuyoshi Takato Kazuto Hoshi Atsuhiko Hikita |
| author_facet | Ryoko Inaki Yoshihiko Sato Daisuke Nakamura Yoshiyuki Aikawa Tsuyoshi Takato Kazuto Hoshi Atsuhiko Hikita |
| author_sort | Ryoko Inaki |
| collection | DOAJ |
| description | Background: Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as temperature, time, air pressure, and vibration affect the cell functions isolated from body tissues. In particular, temperature is a critical factor which determines the viability of the cells and organs. In this study, we investigated the optimal temperature during the transportation of lipoaspirates from which adipose -derived stem cells (ASCs) were isolated. Method: Lipoaspirates obtained by liposuctions (lipomatic or vaser method) were transported in four different temperature zones (4, 20, 32, and 37 °C) in a transport container which is electrically controlled to maintain a constant temperature during transport. Stromal vascular fractions (SVFs) were harvested from the lipoaspirate, and the cell number, viability and proliferation rate and the yield of ASCs were examined. In addition, the metabolic state of the cells was examined. Results: ASCs from lipoaspirates transported at high temperature significantly decreased cell viability, while those at low temperature maintained high cell viability and showed good cell proliferation. In addition, transportation of lipoaspirates at low temperature resulted in a high level of NAD+/NADH, coenzymes involved in intracellular metabolism, and a low level of lactate in lipoaspirate suppressed the glycolytic system of intracellular metabolism, in ASCs. Conclusion: The lipoaspirate transported at 4 °C exhibited best results regarding live cell number, viability and cell proliferation in our experiments. This study offers a direction to build a transport system that connects laboratories and hospitals and achieve a beneficial therapy for patients. |
| first_indexed | 2024-03-09T14:04:53Z |
| format | Article |
| id | doaj.art-77cebaef1eec4294a7b3dd9c369ed900 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-03-09T14:04:53Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-77cebaef1eec4294a7b3dd9c369ed9002023-11-30T05:07:57ZengElsevierRegenerative Therapy2352-32042023-12-0124662669Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viabilityRyoko Inaki0Yoshihiko Sato1Daisuke Nakamura2Yoshiyuki Aikawa3Tsuyoshi Takato4Kazuto Hoshi5Atsuhiko Hikita6Department of Oral-maxillofacial Surgery, Dentistry and Orthodontics, The University of Tokyo Hospital, Tokyo, Japan; Department of Tissue Engineering, The University of Tokyo Hospital, Tokyo, Japan; National Hospital Organization Miyagi National Hospital, JapanPharma & Healthcare Logistics Team, Tokyo Branch, Mitsubishi Logistics Corporation, Tokyo, JapanCosmetic Surgery, SBC Medical Group, Tokyo, JapanCosmetic Surgery, SBC Medical Group, Tokyo, JapanShonan Beauty Clinic- Shinbashi, Tokyo, JapanDepartment of Oral-maxillofacial Surgery, Dentistry and Orthodontics, The University of Tokyo Hospital, Tokyo, Japan; Department of Tissue Engineering, The University of Tokyo Hospital, Tokyo, JapanDepartment of Tissue Engineering, The University of Tokyo Hospital, Tokyo, Japan; Corresponding author. Department of Tissue Engineering, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.Background: Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as temperature, time, air pressure, and vibration affect the cell functions isolated from body tissues. In particular, temperature is a critical factor which determines the viability of the cells and organs. In this study, we investigated the optimal temperature during the transportation of lipoaspirates from which adipose -derived stem cells (ASCs) were isolated. Method: Lipoaspirates obtained by liposuctions (lipomatic or vaser method) were transported in four different temperature zones (4, 20, 32, and 37 °C) in a transport container which is electrically controlled to maintain a constant temperature during transport. Stromal vascular fractions (SVFs) were harvested from the lipoaspirate, and the cell number, viability and proliferation rate and the yield of ASCs were examined. In addition, the metabolic state of the cells was examined. Results: ASCs from lipoaspirates transported at high temperature significantly decreased cell viability, while those at low temperature maintained high cell viability and showed good cell proliferation. In addition, transportation of lipoaspirates at low temperature resulted in a high level of NAD+/NADH, coenzymes involved in intracellular metabolism, and a low level of lactate in lipoaspirate suppressed the glycolytic system of intracellular metabolism, in ASCs. Conclusion: The lipoaspirate transported at 4 °C exhibited best results regarding live cell number, viability and cell proliferation in our experiments. This study offers a direction to build a transport system that connects laboratories and hospitals and achieve a beneficial therapy for patients.http://www.sciencedirect.com/science/article/pii/S2352320423001104LipoaspirateTransportationTemperatureAdipose-derived stem cells |
| spellingShingle | Ryoko Inaki Yoshihiko Sato Daisuke Nakamura Yoshiyuki Aikawa Tsuyoshi Takato Kazuto Hoshi Atsuhiko Hikita Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability Regenerative Therapy Lipoaspirate Transportation Temperature Adipose-derived stem cells |
| title | Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability |
| title_full | Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability |
| title_fullStr | Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability |
| title_full_unstemmed | Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability |
| title_short | Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability |
| title_sort | lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability |
| topic | Lipoaspirate Transportation Temperature Adipose-derived stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2352320423001104 |
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