Application of bioluminescence resonance energy transfer assays in primary mouse neuronal cultures

Summary: Bioluminescence resonance energy transfer (BRET) is widely employed for real-time monitoring of G protein-coupled receptor activity, interactions, and trafficking in heterologous cell lines, yet its use in neuronal systems remains limited. Here, we present a protocol to apply BRET assays to...

Celý popis

Podrobná bibliografie
Hlavní autoři: Hanh T.M. Hoang, Kiran George, Mohiuddin Ahmad
Médium: Článek
Jazyk:English
Vydáno: Elsevier 2024-09-01
Edice:STAR Protocols
Témata:
On-line přístup:http://www.sciencedirect.com/science/article/pii/S2666166724003939