Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers
The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content. This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region (SCAR) marker for ra...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917618252 |
| _version_ | 1818739033366331392 |
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| author | Wen-zheng CHAO Chuan-hong TANG Jing-song ZHANG Ling YU Honda Yoichi |
| author_facet | Wen-zheng CHAO Chuan-hong TANG Jing-song ZHANG Ling YU Honda Yoichi |
| author_sort | Wen-zheng CHAO |
| collection | DOAJ |
| description | The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content. This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region (SCAR) marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat (ISSR) fragment (882 bp) from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp. |
| first_indexed | 2024-12-18T01:18:24Z |
| format | Article |
| id | doaj.art-77d1005a543148588809fc42242f550b |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:18:24Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-77d1005a543148588809fc42242f550b2022-12-21T21:25:54ZengElsevierJournal of Integrative Agriculture2095-31192018-01-01171130138Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markersWen-zheng CHAO0Chuan-hong TANG1Jing-song ZHANG2Ling YU3Honda Yoichi4School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, P.R.China; Key Laboratory of Edible Fungus Resources and Utilization (South), Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.ChinaKey Laboratory of Edible Fungus Resources and Utilization (South), Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.China; Laboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan; Correspondence TANG Chuan-hongKey Laboratory of Edible Fungus Resources and Utilization (South), Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.China; ZHANG Jing-song, Tel: +86-21-62200746, Fax: +86-21-62201530School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, P.R.ChinaLaboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, JapanThe cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content. This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region (SCAR) marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat (ISSR) fragment (882 bp) from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp.http://www.sciencedirect.com/science/article/pii/S2095311917618252DNA poolingGanoderma lucidumHunong 5 cultivarISSR markerSCAR marker |
| spellingShingle | Wen-zheng CHAO Chuan-hong TANG Jing-song ZHANG Ling YU Honda Yoichi Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers Journal of Integrative Agriculture DNA pooling Ganoderma lucidum Hunong 5 cultivar ISSR marker SCAR marker |
| title | Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers |
| title_full | Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers |
| title_fullStr | Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers |
| title_full_unstemmed | Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers |
| title_short | Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers |
| title_sort | development of a stable scar marker for rapid identification of ganoderma lucidum hunong 5 cultivar using dna pooling method and inter simple sequence repeat markers |
| topic | DNA pooling Ganoderma lucidum Hunong 5 cultivar ISSR marker SCAR marker |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917618252 |
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