Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells
Background and Objective: Research have been focused on the applying the chemical inducer for trans-differentiation the adult BMSCs into neural cell. So that, at the first should investigate the toxcity effect of the chemical inducer on the induced cells. Plasticity and easy accessibility of bone ma...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Golestan University of Medical Sciences
2012-08-01
|
| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-600&slc_lang=en&sid=1 |
| _version_ | 1811257995901796352 |
|---|---|
| author | Taheri F Haji Ghasem Kashani M Ghorbanian MT Hosseinpour L |
| author_facet | Taheri F Haji Ghasem Kashani M Ghorbanian MT Hosseinpour L |
| author_sort | Taheri F |
| collection | DOAJ |
| description | Background and Objective: Research have been focused on the applying the chemical inducer for trans-differentiation the adult BMSCs into neural cell. So that, at the first should investigate the toxcity effect of the chemical inducer on the induced cells. Plasticity and easy accessibility of bone marrow mesenchymal stem cells is a unique charactristic for treatment of neural disorderies. This study was desgined to determine the inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells. Materials and Methods: In this experimental study, BMSCs isolated from the adult rat bone marrow and cultured in αMEM containing 10% FBS. Cell identity for surface antigens was performed in third passage by immunocytochemistry and multipotancy capacity of BMSCs was done by BMSC differentiation into adipocytes and osteocytes. The cells were exposed to chemical agents (a: the αMEM medium supplemented with 2% DMSO, b: the αMEM medium supplemented with 10-8M Deprenyl) for 24 houres and then transferred to αMEM containing 10% FBS cell survival and proliferation was evaluated after the 24, 48, 72 and 96 houres by MTT [3-(4-5-Dimethylthiazolyl-2-y1)-2,5-diphenyltetrazolium bromid] test. Data were analyzed using SPSS-16, One-Way ANOVA and Tukey tests. Results: In addition to expression the surface antigens and adipogenic and osteogenic differentiation by BMSCs, MTT test results showed that proliferation and survival of induced-deprenyl and DMSO cells within 48, 72 and 96 hours after the induction was increased significantly than negative control group. Conclusion: Deprenyl increases survival and cell proliferation compared to Dimethyl Sulfoxide. It can be used as cell inducer. |
| first_indexed | 2024-04-12T18:06:18Z |
| format | Article |
| id | doaj.art-77d23404b96446a7bdefe5e842555edb |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-04-12T18:06:18Z |
| publishDate | 2012-08-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-77d23404b96446a7bdefe5e842555edb2022-12-22T03:21:59ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802012-08-011431018Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cellsTaheri F0Haji Ghasem Kashani M1Ghorbanian MT2Hosseinpour L3 MSc Student in Developmental Biology, School of Biology, Damghan University, Damghan, Iran Assistant Professor, Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran Assistant Professor, Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran MSc in Developmental Biology, Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran Background and Objective: Research have been focused on the applying the chemical inducer for trans-differentiation the adult BMSCs into neural cell. So that, at the first should investigate the toxcity effect of the chemical inducer on the induced cells. Plasticity and easy accessibility of bone marrow mesenchymal stem cells is a unique charactristic for treatment of neural disorderies. This study was desgined to determine the inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells. Materials and Methods: In this experimental study, BMSCs isolated from the adult rat bone marrow and cultured in αMEM containing 10% FBS. Cell identity for surface antigens was performed in third passage by immunocytochemistry and multipotancy capacity of BMSCs was done by BMSC differentiation into adipocytes and osteocytes. The cells were exposed to chemical agents (a: the αMEM medium supplemented with 2% DMSO, b: the αMEM medium supplemented with 10-8M Deprenyl) for 24 houres and then transferred to αMEM containing 10% FBS cell survival and proliferation was evaluated after the 24, 48, 72 and 96 houres by MTT [3-(4-5-Dimethylthiazolyl-2-y1)-2,5-diphenyltetrazolium bromid] test. Data were analyzed using SPSS-16, One-Way ANOVA and Tukey tests. Results: In addition to expression the surface antigens and adipogenic and osteogenic differentiation by BMSCs, MTT test results showed that proliferation and survival of induced-deprenyl and DMSO cells within 48, 72 and 96 hours after the induction was increased significantly than negative control group. Conclusion: Deprenyl increases survival and cell proliferation compared to Dimethyl Sulfoxide. It can be used as cell inducer.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-600&slc_lang=en&sid=1Bone marrow mesenchymal stem cellDeprenylDimethyl SulfoxideMTT |
| spellingShingle | Taheri F Haji Ghasem Kashani M Ghorbanian MT Hosseinpour L Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells مجله دانشگاه علوم پزشکی گرگان Bone marrow mesenchymal stem cell Deprenyl Dimethyl Sulfoxide MTT |
| title | Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells |
| title_full | Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells |
| title_fullStr | Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells |
| title_full_unstemmed | Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells |
| title_short | Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells |
| title_sort | inductive effect of deprenyl and dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells |
| topic | Bone marrow mesenchymal stem cell Deprenyl Dimethyl Sulfoxide MTT |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-600&slc_lang=en&sid=1 |
| work_keys_str_mv | AT taherif inductiveeffectofdeprenylanddimethylsulfoxideonproliferationandsurvivalofthemesenchymalstemcells AT hajighasemkashanim inductiveeffectofdeprenylanddimethylsulfoxideonproliferationandsurvivalofthemesenchymalstemcells AT ghorbanianmt inductiveeffectofdeprenylanddimethylsulfoxideonproliferationandsurvivalofthemesenchymalstemcells AT hosseinpourl inductiveeffectofdeprenylanddimethylsulfoxideonproliferationandsurvivalofthemesenchymalstemcells |