CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1
<p>Abstract</p> <p>Background</p> <p>The CXC chemokine ligand 12 (CXCL12)/stromal cell-derived factor-1 (SDF-1) and CXC receptor 4 (CXCR4) axis is involved in human colorectal cancer (CRC) carcinogenesis and can promote the progression of CRC. Interaction between CRC ce...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Journal of Biomedical Science |
| Subjects: | |
| Online Access: | http://www.jbiomedsci.com/content/19/1/91 |
| _version_ | 1819117800046723072 |
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| author | Tung Shui-Yi Chang Shun-Fu Chou Ming-Hui Huang Wen-Shih Hsieh Yung-Yu Shen Chien-Heng Kuo Hsing-Chun Chen Cheng-Nan |
| author_facet | Tung Shui-Yi Chang Shun-Fu Chou Ming-Hui Huang Wen-Shih Hsieh Yung-Yu Shen Chien-Heng Kuo Hsing-Chun Chen Cheng-Nan |
| author_sort | Tung Shui-Yi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The CXC chemokine ligand 12 (CXCL12)/stromal cell-derived factor-1 (SDF-1) and CXC receptor 4 (CXCR4) axis is involved in human colorectal cancer (CRC) carcinogenesis and can promote the progression of CRC. Interaction between CRC cells and endothelium is a key event in tumor progression. The aim of this study was to investigate the effect of SDF-1 on the adhesion of CRC cells.</p> <p>Methods</p> <p>Human CRC DLD-1 cells were used to study the effect of SDF-1 on intercellular adhesion molecule-1 (ICAM-1) expression and cell adhesion to endothelium.</p> <p>Results</p> <p>SDF-1 treatment induced adhesion of DLD-1 cells to the endothelium and increased the expression level of the ICAM-1. Inhibition of ICAM-1 by small interfering RNA (siRNA) and neutralizing antibody inhibited SDF-1-induced cell adhesion. By using specific inhibitors and short hairpin RNA (shRNA), we demonstrated that the activation of ERK, JNK and p38 pathways is critical for SDF-1-induced ICAM-1 expression and cell adhesion. Promoter activity and transcription factor ELISA assays showed that SDF-1 increased Sp1-, C/EBP-β- and NF-κB-DNA binding activities in DLD-1 cells. Inhibition of Sp1, C/EBP-β and NF-κB activations by specific siRNA blocked the SDF-1-induced ICAM-1 promoter activity and expression. The effect of SDF-1 on cell adhesion was mediated by the CXCR4.</p> <p>Conclusion</p> <p>Our findings support the hypothesis that ICAM-1 up-regulation stimulated by SDF-1 may play an active role in CRC cell adhesion.</p> |
| first_indexed | 2024-12-22T05:38:44Z |
| format | Article |
| id | doaj.art-77d5e47a1a6f4c22b8078f8ecebfd648 |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-12-22T05:38:44Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
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| series | Journal of Biomedical Science |
| spelling | doaj.art-77d5e47a1a6f4c22b8078f8ecebfd6482022-12-21T18:37:16ZengBMCJournal of Biomedical Science1021-77701423-01272012-10-011919110.1186/1423-0127-19-91CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1Tung Shui-YiChang Shun-FuChou Ming-HuiHuang Wen-ShihHsieh Yung-YuShen Chien-HengKuo Hsing-ChunChen Cheng-Nan<p>Abstract</p> <p>Background</p> <p>The CXC chemokine ligand 12 (CXCL12)/stromal cell-derived factor-1 (SDF-1) and CXC receptor 4 (CXCR4) axis is involved in human colorectal cancer (CRC) carcinogenesis and can promote the progression of CRC. Interaction between CRC cells and endothelium is a key event in tumor progression. The aim of this study was to investigate the effect of SDF-1 on the adhesion of CRC cells.</p> <p>Methods</p> <p>Human CRC DLD-1 cells were used to study the effect of SDF-1 on intercellular adhesion molecule-1 (ICAM-1) expression and cell adhesion to endothelium.</p> <p>Results</p> <p>SDF-1 treatment induced adhesion of DLD-1 cells to the endothelium and increased the expression level of the ICAM-1. Inhibition of ICAM-1 by small interfering RNA (siRNA) and neutralizing antibody inhibited SDF-1-induced cell adhesion. By using specific inhibitors and short hairpin RNA (shRNA), we demonstrated that the activation of ERK, JNK and p38 pathways is critical for SDF-1-induced ICAM-1 expression and cell adhesion. Promoter activity and transcription factor ELISA assays showed that SDF-1 increased Sp1-, C/EBP-β- and NF-κB-DNA binding activities in DLD-1 cells. Inhibition of Sp1, C/EBP-β and NF-κB activations by specific siRNA blocked the SDF-1-induced ICAM-1 promoter activity and expression. The effect of SDF-1 on cell adhesion was mediated by the CXCR4.</p> <p>Conclusion</p> <p>Our findings support the hypothesis that ICAM-1 up-regulation stimulated by SDF-1 may play an active role in CRC cell adhesion.</p>http://www.jbiomedsci.com/content/19/1/91Colorectal cancerStromal cell-derived factor-1Intercellular adhesion molecule-1Cell adhesionTranscriptional regulation |
| spellingShingle | Tung Shui-Yi Chang Shun-Fu Chou Ming-Hui Huang Wen-Shih Hsieh Yung-Yu Shen Chien-Heng Kuo Hsing-Chun Chen Cheng-Nan CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1 Journal of Biomedical Science Colorectal cancer Stromal cell-derived factor-1 Intercellular adhesion molecule-1 Cell adhesion Transcriptional regulation |
| title | CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1 |
| title_full | CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1 |
| title_fullStr | CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1 |
| title_full_unstemmed | CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1 |
| title_short | CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1 |
| title_sort | cxc chemokine ligand 12 stromal cell derived factor 1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule 1 |
| topic | Colorectal cancer Stromal cell-derived factor-1 Intercellular adhesion molecule-1 Cell adhesion Transcriptional regulation |
| url | http://www.jbiomedsci.com/content/19/1/91 |
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