Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon
AbstractGenes belonging to the same operon are transcribed as a single mRNA molecule in all prokaryotes. The genes of the same operon are presumed to be involved in similar metabolic and physiological processes. Hence, computational analysis of constituent proteins could provide important clues to t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00351/full |
| _version_ | 1818194130987122688 |
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| author | Khurram eMushtaq Javaid Ahmad Sheikh Mohammed eAmir Nargis eKhan Balvinder eSingh Javed Naim Agrewala |
| author_facet | Khurram eMushtaq Javaid Ahmad Sheikh Mohammed eAmir Nargis eKhan Balvinder eSingh Javed Naim Agrewala |
| author_sort | Khurram eMushtaq |
| collection | DOAJ |
| description | AbstractGenes belonging to the same operon are transcribed as a single mRNA molecule in all prokaryotes. The genes of the same operon are presumed to be involved in similar metabolic and physiological processes. Hence, computational analysis of constituent proteins could provide important clues to the functional relationships within the operonic genes. This tends to be more fruitful in the case of Mycobacterium tuberculosis (Mtb), considering the number of hypothetical genes with unknown functions and interacting partners. Dramatic advances in the past decade have increased our knowledge of the mechanisms that tubercle bacilli employ to survive within the host. But the phenomenon of Mtb latency continues to baffle all. Rv2031c belonging to dormancy regulon of Mtb is predominantly expressed during latency, with myriad immunological roles. Thus we attempted to analyze the operon comprising Rv2031c protein to gain insights into its role during latency. In the current study, we have carried out computational analysis of proteins encoded by genes known to be a part of this operon. Our study includes phylogenetic analysis, modeling of protein 3D structures, and protein interaction network analysis. We describe the mechanistic role in the establishment of latency and regulation of DevS/DevR component system. Additionally, we have identified the probable role of these proteins in carbohydrate metabolism, erythromycin tolerance and nucleotide synthesis. Hence, these proteins can modulate the metabolism of mycobacterium inside the host cells and can be important for its survival in latency. The functional characterization and interactome of this important operon can give insight into its role during latency along with the exploitation of constituent proteins as drug targets and vaccine candidates. |
| first_indexed | 2024-12-12T00:57:24Z |
| format | Article |
| id | doaj.art-77d7016ea8b34b299cc3227466ad8a8d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T00:57:24Z |
| publishDate | 2015-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-77d7016ea8b34b299cc3227466ad8a8d2022-12-22T00:43:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-04-01610.3389/fmicb.2015.00351133028Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operonKhurram eMushtaq0Javaid Ahmad Sheikh1Mohammed eAmir2Nargis eKhan3Balvinder eSingh4Javed Naim Agrewala5CSIR- Institute of Microbial TechnologyCSIR- Institute of Microbial TechnologyCSIR- Institute of Microbial TechnologyCSIR- Institute of Microbial TechnologyCSIR- Institute of Microbial TechnologyCSIR- Institute of Microbial TechnologyAbstractGenes belonging to the same operon are transcribed as a single mRNA molecule in all prokaryotes. The genes of the same operon are presumed to be involved in similar metabolic and physiological processes. Hence, computational analysis of constituent proteins could provide important clues to the functional relationships within the operonic genes. This tends to be more fruitful in the case of Mycobacterium tuberculosis (Mtb), considering the number of hypothetical genes with unknown functions and interacting partners. Dramatic advances in the past decade have increased our knowledge of the mechanisms that tubercle bacilli employ to survive within the host. But the phenomenon of Mtb latency continues to baffle all. Rv2031c belonging to dormancy regulon of Mtb is predominantly expressed during latency, with myriad immunological roles. Thus we attempted to analyze the operon comprising Rv2031c protein to gain insights into its role during latency. In the current study, we have carried out computational analysis of proteins encoded by genes known to be a part of this operon. Our study includes phylogenetic analysis, modeling of protein 3D structures, and protein interaction network analysis. We describe the mechanistic role in the establishment of latency and regulation of DevS/DevR component system. Additionally, we have identified the probable role of these proteins in carbohydrate metabolism, erythromycin tolerance and nucleotide synthesis. Hence, these proteins can modulate the metabolism of mycobacterium inside the host cells and can be important for its survival in latency. The functional characterization and interactome of this important operon can give insight into its role during latency along with the exploitation of constituent proteins as drug targets and vaccine candidates.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00351/fullLatent Tuberculosismolecular dockingphylogenetic analysisProtein modellingprotein interaction network analysis |
| spellingShingle | Khurram eMushtaq Javaid Ahmad Sheikh Mohammed eAmir Nargis eKhan Balvinder eSingh Javed Naim Agrewala Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon Frontiers in Microbiology Latent Tuberculosis molecular docking phylogenetic analysis Protein modelling protein interaction network analysis |
| title | Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon |
| title_full | Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon |
| title_fullStr | Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon |
| title_full_unstemmed | Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon |
| title_short | Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon |
| title_sort | rv2031c of mycobacterium tuberculosis a master regulator of rv2028 rv2031 hspx operon |
| topic | Latent Tuberculosis molecular docking phylogenetic analysis Protein modelling protein interaction network analysis |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00351/full |
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