Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology
The paper presents the results of investigation of immunotoxic action of interferon α-2b, immobilized in polyethylene glycol by means of electron-beam synthesis technology.Materials and methods. In this study we used following methods: a preliminary assessment of immunotoxicity after single administ...
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| Format: | Article |
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Siberian State Medical University (Tomsk)
2018-01-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1032 |
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| author | D. N. Kinsht P. G. Madonov E. Yu. Sherstoboev M. G. Danilets E. S. Trofimova A. A. Ligacheva A. M. Dygai |
| author_facet | D. N. Kinsht P. G. Madonov E. Yu. Sherstoboev M. G. Danilets E. S. Trofimova A. A. Ligacheva A. M. Dygai |
| author_sort | D. N. Kinsht |
| collection | DOAJ |
| description | The paper presents the results of investigation of immunotoxic action of interferon α-2b, immobilized in polyethylene glycol by means of electron-beam synthesis technology.Materials and methods. In this study we used following methods: a preliminary assessment of immunotoxicity after single administration in wide range of doses, a study of the effect of the drug on the weight and cellularity of central and peripheral immune organs, an assessment of the phagocytic activity of peritoneal macrophages, a study of the effect of the drug on the phagocytic activity of neutrophils, an estimation of the level of serum hemagglutinins to sheep erythrocytes, an evaluation of the effect of the drug on the delayed-type hypersensitivity reaction induced by trinitrobenzenesulfonic acid.Results. We have shown that the course application of the drug based on immobilized interferon α-2b in tenfold and hundredfold therapeutic doses has no immunotoxic action. At the same time, single administration of the drug at various doses and the therapeutic course application in tenfold dose leads to the stimulation of humoral immune responses. The course application of this drug in tenfold and hundredfold therapeutic dose enhances the phagocytic activity of peritoneal macrophages and peripheral blood neutrophils of experimental animals. |
| first_indexed | 2024-04-10T01:06:26Z |
| format | Article |
| id | doaj.art-77dd7e0e78a84c018c2c695856ec1290 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:06:26Z |
| publishDate | 2018-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-77dd7e0e78a84c018c2c695856ec12902023-03-13T09:58:23ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842018-01-0116414415410.20538/1682-0363-2017-4-144-154740Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technologyD. N. Kinsht0P. G. Madonov1E. Yu. Sherstoboev2M. G. Danilets3E. S. Trofimova4A. A. Ligacheva5A. M. Dygai6Новосибирский государственный медицинский университет (НГМУ)Новосибирский государственный медицинский университет (НГМУ)Научно-исследовательский институт фармакологии и регенеративной медицины (НИИФиРМ) имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр (ТНИМЦ) Российской академии наук (РАН)Научно-исследовательский институт фармакологии и регенеративной медицины (НИИФиРМ) имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр (ТНИМЦ) Российской академии наук (РАН)Научно-исследовательский институт фармакологии и регенеративной медицины (НИИФиРМ) имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр (ТНИМЦ) Российской академии наук (РАН)Научно-исследовательский институт фармакологии и регенеративной медицины (НИИФиРМ) имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр (ТНИМЦ) Российской академии наук (РАН)Научно-исследовательский институт фармакологии и регенеративной медицины (НИИФиРМ) имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр (ТНИМЦ) Российской академии наук (РАН)The paper presents the results of investigation of immunotoxic action of interferon α-2b, immobilized in polyethylene glycol by means of electron-beam synthesis technology.Materials and methods. In this study we used following methods: a preliminary assessment of immunotoxicity after single administration in wide range of doses, a study of the effect of the drug on the weight and cellularity of central and peripheral immune organs, an assessment of the phagocytic activity of peritoneal macrophages, a study of the effect of the drug on the phagocytic activity of neutrophils, an estimation of the level of serum hemagglutinins to sheep erythrocytes, an evaluation of the effect of the drug on the delayed-type hypersensitivity reaction induced by trinitrobenzenesulfonic acid.Results. We have shown that the course application of the drug based on immobilized interferon α-2b in tenfold and hundredfold therapeutic doses has no immunotoxic action. At the same time, single administration of the drug at various doses and the therapeutic course application in tenfold dose leads to the stimulation of humoral immune responses. The course application of this drug in tenfold and hundredfold therapeutic dose enhances the phagocytic activity of peritoneal macrophages and peripheral blood neutrophils of experimental animals.https://bulletin.ssmu.ru/jour/article/view/1032интерферон альфа-2bпегилированиеиммунотоксичностьгуморальный и клеточный иммунный ответфагоцитозмыши-гибриды линии f1(cba×c57bl/6) |
| spellingShingle | D. N. Kinsht P. G. Madonov E. Yu. Sherstoboev M. G. Danilets E. S. Trofimova A. A. Ligacheva A. M. Dygai Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology Бюллетень сибирской медицины интерферон альфа-2b пегилирование иммунотоксичность гуморальный и клеточный иммунный ответ фагоцитоз мыши-гибриды линии f1(cba×c57bl/6) |
| title | Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology |
| title_full | Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology |
| title_fullStr | Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology |
| title_full_unstemmed | Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology |
| title_short | Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology |
| title_sort | immunotoxic effects of interferon alpha 2b modified by immobilization by means of electron beam synthesis technology |
| topic | интерферон альфа-2b пегилирование иммунотоксичность гуморальный и клеточный иммунный ответ фагоцитоз мыши-гибриды линии f1(cba×c57bl/6) |
| url | https://bulletin.ssmu.ru/jour/article/view/1032 |
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