A Rapid Method for Isolating Single Cells from Apple Flesh
The identification, separation and analysis of individual living cells can be used to analyze the heterogeneity and operation mechanism of living systems. The study of fruit development is based on the extraction of active single cells. In this study, we investigated the effects of different enzymes...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2017-03-01
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| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014117301735 |
| _version_ | 1797203302855737344 |
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| author | Yaqin GUAN Haiyong QU |
| author_facet | Yaqin GUAN Haiyong QU |
| author_sort | Yaqin GUAN |
| collection | DOAJ |
| description | The identification, separation and analysis of individual living cells can be used to analyze the heterogeneity and operation mechanism of living systems. The study of fruit development is based on the extraction of active single cells. In this study, we investigated the effects of different enzymes and enzymatic hydrolysis times on the extraction of single cells from the ‘Fuji’ apple (Malus × domestica Borkh. ‘Fuji’). The results showed that the extraction of single cells in apple flesh was a suitable method when 0.1% macerozyme was used and the enzymolysis time was 0.5 h. Fluorescent brightening agent VBL staining showed that the cell wall was intact, while fluorescein diacetate FDA and azo dye Evans blue staining indicated that the extracted single cells were active. The extracted single cells could be further used as materials for protoplast extraction. |
| first_indexed | 2024-04-24T08:17:11Z |
| format | Article |
| id | doaj.art-77e11087a1b2422899ebc2c766f2bf57 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:17:11Z |
| publishDate | 2017-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-77e11087a1b2422899ebc2c766f2bf572024-04-17T03:00:47ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412017-03-0132475210.1016/j.hpj.2017.07.005A Rapid Method for Isolating Single Cells from Apple FleshYaqin GUANHaiyong QUThe identification, separation and analysis of individual living cells can be used to analyze the heterogeneity and operation mechanism of living systems. The study of fruit development is based on the extraction of active single cells. In this study, we investigated the effects of different enzymes and enzymatic hydrolysis times on the extraction of single cells from the ‘Fuji’ apple (Malus × domestica Borkh. ‘Fuji’). The results showed that the extraction of single cells in apple flesh was a suitable method when 0.1% macerozyme was used and the enzymolysis time was 0.5 h. Fluorescent brightening agent VBL staining showed that the cell wall was intact, while fluorescein diacetate FDA and azo dye Evans blue staining indicated that the extracted single cells were active. The extracted single cells could be further used as materials for protoplast extraction.http://www.sciencedirect.com/science/article/pii/S2468014117301735applefleshsingle cellsenzyme liquid concentrationenzymolysis time |
| spellingShingle | Yaqin GUAN Haiyong QU A Rapid Method for Isolating Single Cells from Apple Flesh Horticultural Plant Journal apple flesh single cells enzyme liquid concentration enzymolysis time |
| title | A Rapid Method for Isolating Single Cells from Apple Flesh |
| title_full | A Rapid Method for Isolating Single Cells from Apple Flesh |
| title_fullStr | A Rapid Method for Isolating Single Cells from Apple Flesh |
| title_full_unstemmed | A Rapid Method for Isolating Single Cells from Apple Flesh |
| title_short | A Rapid Method for Isolating Single Cells from Apple Flesh |
| title_sort | rapid method for isolating single cells from apple flesh |
| topic | apple flesh single cells enzyme liquid concentration enzymolysis time |
| url | http://www.sciencedirect.com/science/article/pii/S2468014117301735 |
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