Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability
Abstract Background The Prototheca algae have recently emerged as an important cause of bovine mastitis globally. Isolates from bovine mastitis in several countries were nearly all identified as P. bovis, suggesting that it was the main causative agent of bovine protothecal mastitis. The aim of the...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03546-4 |
| _version_ | 1797973569107394560 |
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| author | Niloufar Tashakkori Hamideh Kalateh Rahmani Babak Khoramian |
| author_facet | Niloufar Tashakkori Hamideh Kalateh Rahmani Babak Khoramian |
| author_sort | Niloufar Tashakkori |
| collection | DOAJ |
| description | Abstract Background The Prototheca algae have recently emerged as an important cause of bovine mastitis globally. Isolates from bovine mastitis in several countries were nearly all identified as P. bovis, suggesting that it was the main causative agent of bovine protothecal mastitis. The aim of the present study was to evaluate the presence and isolation of Prototheca spp. in dairy farms, detect the genetic diversity among strains, determine the capacity of producing biofilm and their resistance to antifungal and antimicrobial drugs. Results A total of 48 Prototheca isolates from four different farms were randomly selected to be investigated. Multiplex PCR showed all isolated colonies were Prototheca bovis. Performing RAPD-PCR by using OPA-4 primer, it was revealed that there was a clear amplification pattern. Different levels of biofilm production were observed among strains. Among 48 isolates, only 4 of them (8.33%) showed strong biofilm production. By using E-test strips, amphotericin B was able to inhibit the growth of all the strains tested. Disc diffusion method used for antimicrobial sensitivity test showed that the highest activity was demonstrated by gentamicin and colistin with 95.83% (46/48) and 89.58% (43/48) of sensitive strains, respectively. Conclusions The present study showed that RAPD-PCR was a rapid tool for discriminating P. bovis strains. Also, gentamicin and colistin can be considered as potential antimicrobial drugs which can prevent the growth of the mentioned strains in vitro, although there is no effective clinical treatment yet. Further studies are needed in order to detect an effective clinical therapy considering biofilm production by Prototheca spp. and their probable role in Prototheca pathogenicity. |
| first_indexed | 2024-04-11T04:06:19Z |
| format | Article |
| id | doaj.art-77e564827ca843d6a9de74ecf7244ba0 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T04:06:19Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-77e564827ca843d6a9de74ecf7244ba02023-01-01T12:21:40ZengBMCBMC Veterinary Research1746-61482022-12-011811710.1186/s12917-022-03546-4Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation abilityNiloufar Tashakkori0Hamideh Kalateh Rahmani1Babak Khoramian2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of MashhadDepartment of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of MashhadDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of MashhadAbstract Background The Prototheca algae have recently emerged as an important cause of bovine mastitis globally. Isolates from bovine mastitis in several countries were nearly all identified as P. bovis, suggesting that it was the main causative agent of bovine protothecal mastitis. The aim of the present study was to evaluate the presence and isolation of Prototheca spp. in dairy farms, detect the genetic diversity among strains, determine the capacity of producing biofilm and their resistance to antifungal and antimicrobial drugs. Results A total of 48 Prototheca isolates from four different farms were randomly selected to be investigated. Multiplex PCR showed all isolated colonies were Prototheca bovis. Performing RAPD-PCR by using OPA-4 primer, it was revealed that there was a clear amplification pattern. Different levels of biofilm production were observed among strains. Among 48 isolates, only 4 of them (8.33%) showed strong biofilm production. By using E-test strips, amphotericin B was able to inhibit the growth of all the strains tested. Disc diffusion method used for antimicrobial sensitivity test showed that the highest activity was demonstrated by gentamicin and colistin with 95.83% (46/48) and 89.58% (43/48) of sensitive strains, respectively. Conclusions The present study showed that RAPD-PCR was a rapid tool for discriminating P. bovis strains. Also, gentamicin and colistin can be considered as potential antimicrobial drugs which can prevent the growth of the mentioned strains in vitro, although there is no effective clinical treatment yet. Further studies are needed in order to detect an effective clinical therapy considering biofilm production by Prototheca spp. and their probable role in Prototheca pathogenicity.https://doi.org/10.1186/s12917-022-03546-4ProtothecaMolecular typingRAPD-PCRBiofilmAntimicrobialAntifungal |
| spellingShingle | Niloufar Tashakkori Hamideh Kalateh Rahmani Babak Khoramian Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability BMC Veterinary Research Prototheca Molecular typing RAPD-PCR Biofilm Antimicrobial Antifungal |
| title | Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability |
| title_full | Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability |
| title_fullStr | Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability |
| title_full_unstemmed | Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability |
| title_short | Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability |
| title_sort | genotypic and phenotypic diversity of prototheca spp recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability |
| topic | Prototheca Molecular typing RAPD-PCR Biofilm Antimicrobial Antifungal |
| url | https://doi.org/10.1186/s12917-022-03546-4 |
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