A Streamlined Approach for Fluorescence Labelling of Low-Copy-Number Plasmids for Determination of Conjugation Frequency by Flow Cytometry

Bacterial conjugation plays a major role in the dissemination of antibiotic resistance and virulence traits through horizontal transfer of plasmids. Robust measurement of conjugation frequency of plasmids between bacterial strains and species is therefore important for understanding the transfer dyn...

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Bibliographic Details
Main Authors: Qin Qi, Muhammad Kamruzzaman, Jonathan R. Iredell
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/11/4/878