Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle
In order to investigate the effect of Bacillus amyloliquefaciens GSBa-1 on polyphenol synthesis and phenylpropanoid metabolism in Citrus medica L. var. sarcodactylis Swingle stored at normal temperature, C. medica L. var. sarcodactylis Swingle was soaked in a 1 × 108 CFU/mL suspension of B. amyloliq...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2024-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-3-021.pdf |
| _version_ | 1797256641328971776 |
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| author | ZHANG Min, LU Qingchen, ZHOU Xinqun, WANG Lihua, SUN Jing, GAO Haina, LIU Bangdi |
| author_facet | ZHANG Min, LU Qingchen, ZHOU Xinqun, WANG Lihua, SUN Jing, GAO Haina, LIU Bangdi |
| author_sort | ZHANG Min, LU Qingchen, ZHOU Xinqun, WANG Lihua, SUN Jing, GAO Haina, LIU Bangdi |
| collection | DOAJ |
| description | In order to investigate the effect of Bacillus amyloliquefaciens GSBa-1 on polyphenol synthesis and phenylpropanoid metabolism in Citrus medica L. var. sarcodactylis Swingle stored at normal temperature, C. medica L. var. sarcodactylis Swingle was soaked in a 1 × 108 CFU/mL suspension of B. amyloliquefaciens GSBa-1, taken out and air-dried before being stored at room temperature. The contents of total soluble solids (TSS) and titratable acids (TA), ethylene release, respiration intensity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, H2O2 concentration, polyphenol content and enzyme activities related to the phenylpropane metabolic pathway were measured. The results showed that treatment with GSBa-1 reduced ethylene release and respiratory intensity, inhibited peel color change and fruit softening, induced an increase in phenylalanine ammonia lyase (PAL) and cinnamate-4-hydroxylase (C4H) activities, rapidly promoted the synthesis of gallic acid and caffeic acid as representatives of phenolic acids, induced the accumulation of flavonoids and flavanols such as hesperidin, seroxin and diosmin, increased the contents of total phenols and total flavonoids, and improved DPPH radical scavenging capacity and H2O2 content and consequently fruit resistance. It can be concluded that B. amylolytic GSBa-1 can activate the phenylpropane metabolic pathway, and stimulate the synthesis and accumulation of polyphenols, thereby delaying post-ripening and senescence and improving storage quality of C. medica L. var. sarcodactylis Swingle. |
| first_indexed | 2024-04-24T22:24:58Z |
| format | Article |
| id | doaj.art-77e711a2b6b743f9b6a56394a97845a8 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-24T22:24:58Z |
| publishDate | 2024-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-77e711a2b6b743f9b6a56394a97845a82024-03-20T02:27:12ZengChina Food Publishing CompanyShipin Kexue1002-66302024-02-0145316717510.7506/spkx1002-6630-20230319-184Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis SwingleZHANG Min, LU Qingchen, ZHOU Xinqun, WANG Lihua, SUN Jing, GAO Haina, LIU Bangdi0(1. Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China; 2. Key Laboratory of Agro-products Postharvest Handling, Ministry of Agriculture and Rural Affairs, Beijing 100121, China; 3. School of Life Sciences and Food Engineering, Hebei University of Technology, Handan 056038, China; 4. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China)In order to investigate the effect of Bacillus amyloliquefaciens GSBa-1 on polyphenol synthesis and phenylpropanoid metabolism in Citrus medica L. var. sarcodactylis Swingle stored at normal temperature, C. medica L. var. sarcodactylis Swingle was soaked in a 1 × 108 CFU/mL suspension of B. amyloliquefaciens GSBa-1, taken out and air-dried before being stored at room temperature. The contents of total soluble solids (TSS) and titratable acids (TA), ethylene release, respiration intensity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, H2O2 concentration, polyphenol content and enzyme activities related to the phenylpropane metabolic pathway were measured. The results showed that treatment with GSBa-1 reduced ethylene release and respiratory intensity, inhibited peel color change and fruit softening, induced an increase in phenylalanine ammonia lyase (PAL) and cinnamate-4-hydroxylase (C4H) activities, rapidly promoted the synthesis of gallic acid and caffeic acid as representatives of phenolic acids, induced the accumulation of flavonoids and flavanols such as hesperidin, seroxin and diosmin, increased the contents of total phenols and total flavonoids, and improved DPPH radical scavenging capacity and H2O2 content and consequently fruit resistance. It can be concluded that B. amylolytic GSBa-1 can activate the phenylpropane metabolic pathway, and stimulate the synthesis and accumulation of polyphenols, thereby delaying post-ripening and senescence and improving storage quality of C. medica L. var. sarcodactylis Swingle.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-3-021.pdfbacillus amyloliquefaciens gsba-1; citrus medica l. var. sarcodactylis swingle; polyphenol; phenylpropane metabolism; storage quality |
| spellingShingle | ZHANG Min, LU Qingchen, ZHOU Xinqun, WANG Lihua, SUN Jing, GAO Haina, LIU Bangdi Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle Shipin Kexue bacillus amyloliquefaciens gsba-1; citrus medica l. var. sarcodactylis swingle; polyphenol; phenylpropane metabolism; storage quality |
| title | Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle |
| title_full | Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle |
| title_fullStr | Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle |
| title_full_unstemmed | Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle |
| title_short | Effect of Bacillus amyloliquefaciens GSBa-1 on Polyphenol Synthesis and Phenylpropanoid Metabolism in Citrus medica L. var. sarcodactylis Swingle |
| title_sort | effect of bacillus amyloliquefaciens gsba 1 on polyphenol synthesis and phenylpropanoid metabolism in citrus medica l var sarcodactylis swingle |
| topic | bacillus amyloliquefaciens gsba-1; citrus medica l. var. sarcodactylis swingle; polyphenol; phenylpropane metabolism; storage quality |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-3-021.pdf |
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