Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in <i>Escherichia coli</i> and One-Step Chromatographic Purification

Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in <i>Escherichia coli</i> and One-Step Chromatographic Purification

Although single-chain variable fragment (scFv) is recognized as a highly versatile scaffold of recombinant antibody fragment molecules, its overexpression in <i>Escherichia coli</i> often leads to the formation of inclusion bodies. To address this issue, we devised and tested four differ...

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Bibliographic Details
Main Authors: Kyu Tae Byun, Boram Kim, Junmin Cho, Inbeom Lee, Myung Gu Lee, Dongsun Park, Tae-Bong Kang, Hyung-Sik Won, Chan Gil Kim
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Biomolecules
Subjects:
antibody
single-chain variable fragment (scFv)
human epidermal growth factor receptor 2 (HER2)
bacterial production
<i>Escherichia coli</i>
maltose-binding protein (MBP)
Online Access:https://www.mdpi.com/2218-273X/13/10/1508

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