Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis
Electroacupuncture (EA) and induced pluripotent stem cell (iPSC)-derived small extracellular vesicles (iPSC-EVs) have substantial beneficial effects on ischemic stroke. However, the detailed mechanisms remain unclear. Here, we explored the mechanisms underlying the regulation of EA and iPSC-EVs in t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1107559/full |
| _version_ | 1797946913428865024 |
|---|---|
| author | Qiongqiong Zhang Peiying Deng Suhui Chen Hong Xu Yamin Zhang Hui Chen Hui Chen Hui Chen Jianmin Zhang Jianmin Zhang Jianmin Zhang Jianmin Zhang Hua Sun |
| author_facet | Qiongqiong Zhang Peiying Deng Suhui Chen Hong Xu Yamin Zhang Hui Chen Hui Chen Hui Chen Jianmin Zhang Jianmin Zhang Jianmin Zhang Jianmin Zhang Hua Sun |
| author_sort | Qiongqiong Zhang |
| collection | DOAJ |
| description | Electroacupuncture (EA) and induced pluripotent stem cell (iPSC)-derived small extracellular vesicles (iPSC-EVs) have substantial beneficial effects on ischemic stroke. However, the detailed mechanisms remain unclear. Here, we explored the mechanisms underlying the regulation of EA and iPSC-EVs in the microbiome-gut-brain axis (MGBA) after ischemic stroke. Ischemic stroke mice (C57BL/6) were subjected to middle cerebral artery occlusion (MCAO) or Sham surgery. EA and iPSC-EVs treatments significantly improved neurological function and neuronal and intestinal tract injury, downregulated the levels of IL-17 expression and upregulated IL-10 levels in brain and colon tissue after cerebral ischemia−reperfusion. EA and iPSC-EVs treatments also modulated the microbiota composition and diversity as well as the differential distribution of species in the intestines of the mice after cerebral ischemia−reperfusion. Our results demonstrated that EA and iPSC-EVs treatments regulated intestinal immunity through MGBA regulation of intestinal microbes, reducing brain and colon damage following cerebral ischemia and positively impacting the outcomes of ischemic stroke. Our findings provide new insights into the application of EA combined with iPSC-EVs as a treatment for ischemic stroke. |
| first_indexed | 2024-04-10T21:19:41Z |
| format | Article |
| id | doaj.art-77eacd8b7ff04a8b89fcc1312e14651a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T21:19:41Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-77eacd8b7ff04a8b89fcc1312e14651a2023-01-20T07:46:26ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-01-011410.3389/fimmu.2023.11075591107559Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axisQiongqiong Zhang0Peiying Deng1Suhui Chen2Hong Xu3Yamin Zhang4Hui Chen5Hui Chen6Hui Chen7Jianmin Zhang8Jianmin Zhang9Jianmin Zhang10Jianmin Zhang11Hua Sun12Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaCAMS Key Laboratory for T-Cell and Immunotherapy, State Key Laboratory of Medical Molecular Biology, Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, ChinaHaihe Laboratory of Cell Ecosystem, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, ChinaChangzhou Xitaihu Institute for Frontier Technology of Cell Therapy, Changzhou, ChinaCAMS Key Laboratory for T-Cell and Immunotherapy, State Key Laboratory of Medical Molecular Biology, Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, ChinaHaihe Laboratory of Cell Ecosystem, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, ChinaChangzhou Xitaihu Institute for Frontier Technology of Cell Therapy, Changzhou, ChinaGuidon Pharmaceutics, Inc., Beijing, ChinaDepartment of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaElectroacupuncture (EA) and induced pluripotent stem cell (iPSC)-derived small extracellular vesicles (iPSC-EVs) have substantial beneficial effects on ischemic stroke. However, the detailed mechanisms remain unclear. Here, we explored the mechanisms underlying the regulation of EA and iPSC-EVs in the microbiome-gut-brain axis (MGBA) after ischemic stroke. Ischemic stroke mice (C57BL/6) were subjected to middle cerebral artery occlusion (MCAO) or Sham surgery. EA and iPSC-EVs treatments significantly improved neurological function and neuronal and intestinal tract injury, downregulated the levels of IL-17 expression and upregulated IL-10 levels in brain and colon tissue after cerebral ischemia−reperfusion. EA and iPSC-EVs treatments also modulated the microbiota composition and diversity as well as the differential distribution of species in the intestines of the mice after cerebral ischemia−reperfusion. Our results demonstrated that EA and iPSC-EVs treatments regulated intestinal immunity through MGBA regulation of intestinal microbes, reducing brain and colon damage following cerebral ischemia and positively impacting the outcomes of ischemic stroke. Our findings provide new insights into the application of EA combined with iPSC-EVs as a treatment for ischemic stroke.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1107559/fullelectroacupunctureischemic strokeinduced pluripotent stem cellmicrobiome-gut-brain axisinflammation |
| spellingShingle | Qiongqiong Zhang Peiying Deng Suhui Chen Hong Xu Yamin Zhang Hui Chen Hui Chen Hui Chen Jianmin Zhang Jianmin Zhang Jianmin Zhang Jianmin Zhang Hua Sun Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis Frontiers in Immunology electroacupuncture ischemic stroke induced pluripotent stem cell microbiome-gut-brain axis inflammation |
| title | Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis |
| title_full | Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis |
| title_fullStr | Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis |
| title_full_unstemmed | Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis |
| title_short | Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis |
| title_sort | electroacupuncture and human ipsc derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain gut axis |
| topic | electroacupuncture ischemic stroke induced pluripotent stem cell microbiome-gut-brain axis inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1107559/full |
| work_keys_str_mv | AT qiongqiongzhang electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT peiyingdeng electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT suhuichen electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT hongxu electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT yaminzhang electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT huichen electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT huichen electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT huichen electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT jianminzhang electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT jianminzhang electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT jianminzhang electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT jianminzhang electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis AT huasun electroacupunctureandhumanipscderivedsmallextracellularvesiclesregulatethegutmicrobiotainischemicstrokeviathebraingutaxis |