QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat
Deficiency of micronutrient elements, such as zinc (Zn) and iron (Fe), is called “hidden hunger,” and bio-fortification is the most effective way to overcome the problem. In this study, a high-density Affymetrix 50K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.680391/full |
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| author | Yue Wang Xiaoting Xu Yuanfeng Hao Yelun Zhang Yuping Liu Zongjun Pu Yubing Tian Dengan Xu Xianchun Xia Zhonghu He Zhonghu He Yong Zhang |
| author_facet | Yue Wang Xiaoting Xu Yuanfeng Hao Yelun Zhang Yuping Liu Zongjun Pu Yubing Tian Dengan Xu Xianchun Xia Zhonghu He Zhonghu He Yong Zhang |
| author_sort | Yue Wang |
| collection | DOAJ |
| description | Deficiency of micronutrient elements, such as zinc (Zn) and iron (Fe), is called “hidden hunger,” and bio-fortification is the most effective way to overcome the problem. In this study, a high-density Affymetrix 50K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for grain Zn (GZn) and grain Fe (GFe) concentrations in 254 recombinant inbred lines (RILs) from a cross Jingdong 8/Bainong AK58 in nine environments. There was a wide range of variation in GZn and GFe concentrations among the RILs, with the largest effect contributed by the line × environment interaction, followed by line and environmental effects. The broad sense heritabilities of GZn and GFe were 0.36 ± 0.03 and 0.39 ± 0.03, respectively. Seven QTL for GZn on chromosomes 1DS, 2AS, 3BS, 4DS, 6AS, 6DL, and 7BL accounted for 2.2–25.1% of the phenotypic variances, and four QTL for GFe on chromosomes 3BL, 4DS, 6AS, and 7BL explained 2.3–30.4% of the phenotypic variances. QTL on chromosomes 4DS, 6AS, and 7BL might have pleiotropic effects on both GZn and GFe that were validated on a germplasm panel. Closely linked SNP markers were converted to high-throughput KASP markers, providing valuable tools for selection of improved Zn and Fe bio-fortification in breeding. |
| first_indexed | 2024-12-16T14:39:19Z |
| format | Article |
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| language | English |
| last_indexed | 2024-12-16T14:39:19Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-77eacdbf8a0242e197db647a6319f5222022-12-21T22:28:00ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-06-01810.3389/fnut.2021.680391680391QTL Mapping for Grain Zinc and Iron Concentrations in Bread WheatYue Wang0Xiaoting Xu1Yuanfeng Hao2Yelun Zhang3Yuping Liu4Zongjun Pu5Yubing Tian6Dengan Xu7Xianchun Xia8Zhonghu He9Zhonghu He10Yong Zhang11National Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaHebei Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, ChinaHebei Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, ChinaInstitute of Crop Sciences, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInternational Maize and Wheat Improvement Center (CIMMYT) China Office, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaDeficiency of micronutrient elements, such as zinc (Zn) and iron (Fe), is called “hidden hunger,” and bio-fortification is the most effective way to overcome the problem. In this study, a high-density Affymetrix 50K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for grain Zn (GZn) and grain Fe (GFe) concentrations in 254 recombinant inbred lines (RILs) from a cross Jingdong 8/Bainong AK58 in nine environments. There was a wide range of variation in GZn and GFe concentrations among the RILs, with the largest effect contributed by the line × environment interaction, followed by line and environmental effects. The broad sense heritabilities of GZn and GFe were 0.36 ± 0.03 and 0.39 ± 0.03, respectively. Seven QTL for GZn on chromosomes 1DS, 2AS, 3BS, 4DS, 6AS, 6DL, and 7BL accounted for 2.2–25.1% of the phenotypic variances, and four QTL for GFe on chromosomes 3BL, 4DS, 6AS, and 7BL explained 2.3–30.4% of the phenotypic variances. QTL on chromosomes 4DS, 6AS, and 7BL might have pleiotropic effects on both GZn and GFe that were validated on a germplasm panel. Closely linked SNP markers were converted to high-throughput KASP markers, providing valuable tools for selection of improved Zn and Fe bio-fortification in breeding.https://www.frontiersin.org/articles/10.3389/fnut.2021.680391/fullTriticum aestivummineral biofortificationquantitative trait locus50K SNP arrayKASP marker |
| spellingShingle | Yue Wang Xiaoting Xu Yuanfeng Hao Yelun Zhang Yuping Liu Zongjun Pu Yubing Tian Dengan Xu Xianchun Xia Zhonghu He Zhonghu He Yong Zhang QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat Frontiers in Nutrition Triticum aestivum mineral biofortification quantitative trait locus 50K SNP array KASP marker |
| title | QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat |
| title_full | QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat |
| title_fullStr | QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat |
| title_full_unstemmed | QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat |
| title_short | QTL Mapping for Grain Zinc and Iron Concentrations in Bread Wheat |
| title_sort | qtl mapping for grain zinc and iron concentrations in bread wheat |
| topic | Triticum aestivum mineral biofortification quantitative trait locus 50K SNP array KASP marker |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.680391/full |
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