Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production
Pectinases are important enzymes not only for their potential applications in different industries such animal feed, agricultural, textile, beverage, food processing, oil extraction, etc. Ten fungal species were isolated from the soil and screened for production of pectinase enzyme by using the pect...
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| Format: | Article |
| Language: | English |
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São Paulo State University (UNESP)
2020-06-01
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| Series: | Revista de Ciências Farmacêuticas Básica e Aplicada |
| Subjects: | |
| Online Access: | http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/635 |
| _version_ | 1819049945272942592 |
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| author | Maísa Davanso Abra Eli Atsakou Edwil A. L. Gattás Ariela Veloso de Paula |
| author_facet | Maísa Davanso Abra Eli Atsakou Edwil A. L. Gattás Ariela Veloso de Paula |
| author_sort | Maísa Davanso |
| collection | DOAJ |
| description | Pectinases are important enzymes not only for their potential applications in different industries such animal feed, agricultural, textile, beverage, food processing, oil extraction, etc. Ten fungal species were isolated from the soil and screened for production of pectinase enzyme by using the pectin agar medium. Pectinolytic enzymes synthesis were attained at a temperature of 30 °C and activities were determined after a seven-days culture of Aspergillus sp. 391 and Aspergillus sp. 031, in a basic medium containing 2% citrus pectin and as the sole carbon source. The extract enzymatic showed an optimum activity for exo-polygalacturonase (PG) and pectin lyase (PNL) against galacturonic acid and pectin at pH 4.5 and 5.5, respectively. There were variations in PG and PNL enzymes levels produced in culture filtrates obtained of Aspergillus sp. 391 with addition of citrus waste (2.0 and 4.0 % w/v) to the medium. Maximum activity for PNL activity was observed in the medium containing 5% pectin or 4% citrus waste, as sole carbon source, after 7 days of growth. The results showed that the isolate Aspergillus sp. 391 is a promising for pectinolytic enzymes production at the industrial level. |
| first_indexed | 2024-12-21T11:40:13Z |
| format | Article |
| id | doaj.art-77edeef168a34f9dbe34fe1b71674427 |
| institution | Directory Open Access Journal |
| issn | 1808-4532 2179-443X |
| language | English |
| last_indexed | 2024-12-21T11:40:13Z |
| publishDate | 2020-06-01 |
| publisher | São Paulo State University (UNESP) |
| record_format | Article |
| series | Revista de Ciências Farmacêuticas Básica e Aplicada |
| spelling | doaj.art-77edeef168a34f9dbe34fe1b716744272022-12-21T19:05:19ZengSão Paulo State University (UNESP)Revista de Ciências Farmacêuticas Básica e Aplicada1808-45322179-443X2020-06-014015635Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase productionMaísa Davanso0Abra Eli Atsakou1Edwil A. L. Gattás2Ariela Veloso de Paula3Instituto de Química, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrasilFaculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrasilFaculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrasilFaculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrasilPectinases are important enzymes not only for their potential applications in different industries such animal feed, agricultural, textile, beverage, food processing, oil extraction, etc. Ten fungal species were isolated from the soil and screened for production of pectinase enzyme by using the pectin agar medium. Pectinolytic enzymes synthesis were attained at a temperature of 30 °C and activities were determined after a seven-days culture of Aspergillus sp. 391 and Aspergillus sp. 031, in a basic medium containing 2% citrus pectin and as the sole carbon source. The extract enzymatic showed an optimum activity for exo-polygalacturonase (PG) and pectin lyase (PNL) against galacturonic acid and pectin at pH 4.5 and 5.5, respectively. There were variations in PG and PNL enzymes levels produced in culture filtrates obtained of Aspergillus sp. 391 with addition of citrus waste (2.0 and 4.0 % w/v) to the medium. Maximum activity for PNL activity was observed in the medium containing 5% pectin or 4% citrus waste, as sole carbon source, after 7 days of growth. The results showed that the isolate Aspergillus sp. 391 is a promising for pectinolytic enzymes production at the industrial level.http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/635aspergillus sp.pectinaseenzyme productionactivity |
| spellingShingle | Maísa Davanso Abra Eli Atsakou Edwil A. L. Gattás Ariela Veloso de Paula Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production Revista de Ciências Farmacêuticas Básica e Aplicada aspergillus sp. pectinase enzyme production activity |
| title | Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production |
| title_full | Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production |
| title_fullStr | Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production |
| title_full_unstemmed | Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production |
| title_short | Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production |
| title_sort | assessment of pectinase producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production |
| topic | aspergillus sp. pectinase enzyme production activity |
| url | http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/635 |
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