Optimization of in vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation of soybean for visual screening of transformants using RUBY

Optimization of in vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation of soybean for visual screening of transformants using RUBY

In vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation (HRT) assays are key components of the plant biotechnology and functional genomics toolkit. In this report, both in vitro and ex vitro HRT were optimized in soybean using the RUBY reporter. Different parameters includi...

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Bibliographic Details
Main Authors: Mohsen Niazian, François Belzile, Shaun J. Curtin, Maxime de Ronne, Davoud Torkamaneh
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-07-01
Series:Frontiers in Plant Science
Subjects:
hairy root transformation
gene functional analysis
artificial neural networks
soybean
visual detection
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2023.1207762/full

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