Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients
Diabetes mellitus type 2 (T2DM) is one of the most prevalent non-communicable diseases worldwide. Interleukin-17 (IL-17, or IL-17A) is a crucial cytokine that links T cell activation to neutrophil recruitment and activity. In the cytokine storm, up-regulation of T-helper 17 cell cytokine IL-17A,...
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| Format: | Article |
| Language: | English |
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College of Education for Pure Sciences
2022-09-01
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| Series: | Wasit Journal for Pure Sciences |
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| Online Access: | https://wjps.uowasit.edu.iq/index.php/wjps/article/view/43 |
| _version_ | 1797288373396701184 |
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| author | Marwa Kadhum Intisar Ahmed |
| author_facet | Marwa Kadhum Intisar Ahmed |
| author_sort | Marwa Kadhum |
| collection | DOAJ |
| description |
Diabetes mellitus type 2 (T2DM) is one of the most prevalent
non-communicable diseases worldwide. Interleukin-17 (IL-17, or IL-17A) is a
crucial cytokine that links T cell activation to neutrophil recruitment and activity. In the cytokine storm, up-regulation of T-helper 17 cell cytokine IL-17A, this study aims to investigate the association of polymorphism of IL-17A (rs2275913) gene to their serum levels among Iraqi diabetic type 2 patients. A total of 70 participants, 50 were confirmed previously type 2 diabetic patients, and 20 were apparently healthy. Genetic polymorphism of IL-17AG197A rs (2275913) was carried out using RFLP-PCR. Serum levels of IL-17A were performed using by enzyme-linked immuno-sorbent assay technique (ELISA) using a Human- IL-17A kit. The results of the current study indicated the levels IL-17A were higher in the sera of diabetic patients compared to healthy controls, although there were no significant differences, in patients (243.7233±38.45944), vs. controls (148.8930±11.79280), P= 0.135. . Moreover, T2DM patients with AA and AG genotypes showed higher levels of IL17A than that with GG genotype: 252.5476±50.775 for AA genotype and253.5817±79.13 for AG genotype com-pared to163.5100±8.14105, 115.2900±0.0 for each genotype in controls, P> 0.05.
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| first_indexed | 2024-03-07T18:48:24Z |
| format | Article |
| id | doaj.art-77effa0745aa441da1df96dffb275402 |
| institution | Directory Open Access Journal |
| issn | 2790-5233 2790-5241 |
| language | English |
| last_indexed | 2024-03-07T18:48:24Z |
| publishDate | 2022-09-01 |
| publisher | College of Education for Pure Sciences |
| record_format | Article |
| series | Wasit Journal for Pure Sciences |
| spelling | doaj.art-77effa0745aa441da1df96dffb2754022024-03-02T02:03:37ZengCollege of Education for Pure SciencesWasit Journal for Pure Sciences2790-52332790-52412022-09-011210.31185/wjps.43Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic PatientsMarwa Kadhum0Intisar Ahmed1College of Education for Pure Sciences, Wasit University, IraqCollege of Education for Pure Sciences, Wasit University, Iraq Diabetes mellitus type 2 (T2DM) is one of the most prevalent non-communicable diseases worldwide. Interleukin-17 (IL-17, or IL-17A) is a crucial cytokine that links T cell activation to neutrophil recruitment and activity. In the cytokine storm, up-regulation of T-helper 17 cell cytokine IL-17A, this study aims to investigate the association of polymorphism of IL-17A (rs2275913) gene to their serum levels among Iraqi diabetic type 2 patients. A total of 70 participants, 50 were confirmed previously type 2 diabetic patients, and 20 were apparently healthy. Genetic polymorphism of IL-17AG197A rs (2275913) was carried out using RFLP-PCR. Serum levels of IL-17A were performed using by enzyme-linked immuno-sorbent assay technique (ELISA) using a Human- IL-17A kit. The results of the current study indicated the levels IL-17A were higher in the sera of diabetic patients compared to healthy controls, although there were no significant differences, in patients (243.7233±38.45944), vs. controls (148.8930±11.79280), P= 0.135. . Moreover, T2DM patients with AA and AG genotypes showed higher levels of IL17A than that with GG genotype: 252.5476±50.775 for AA genotype and253.5817±79.13 for AG genotype com-pared to163.5100±8.14105, 115.2900±0.0 for each genotype in controls, P> 0.05. https://wjps.uowasit.edu.iq/index.php/wjps/article/view/43T2DM, IL-17A, RFLP-PCR, ELISA |
| spellingShingle | Marwa Kadhum Intisar Ahmed Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients Wasit Journal for Pure Sciences T2DM, IL-17A, RFLP-PCR, ELISA |
| title | Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients |
| title_full | Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients |
| title_fullStr | Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients |
| title_full_unstemmed | Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients |
| title_short | Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients |
| title_sort | association of interleukin 17a genotypes with their serum levels in iraqi type 2 diabetic patients |
| topic | T2DM, IL-17A, RFLP-PCR, ELISA |
| url | https://wjps.uowasit.edu.iq/index.php/wjps/article/view/43 |
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