First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants
This study investigated polymorphisms in the milk protein genes <i>CSN1S1</i>, <i>CSN2</i>, <i>CSN1S2</i>, <i>CSN3</i>, <i>LALBA</i>, and <i>LGB</i>, and casein haplotypes in Beninese indigenous cattle. Considering 67 animals, D...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/11/1702 |
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| author | Sèyi Fridaïus Ulrich Vanvanhossou Isabella Jasmin Giambra Tong Yin Kerstin Brügemann Luc Hippolyte Dossa Sven König |
| author_facet | Sèyi Fridaïus Ulrich Vanvanhossou Isabella Jasmin Giambra Tong Yin Kerstin Brügemann Luc Hippolyte Dossa Sven König |
| author_sort | Sèyi Fridaïus Ulrich Vanvanhossou |
| collection | DOAJ |
| description | This study investigated polymorphisms in the milk protein genes <i>CSN1S1</i>, <i>CSN2</i>, <i>CSN1S2</i>, <i>CSN3</i>, <i>LALBA</i>, and <i>LGB</i>, and casein haplotypes in Beninese indigenous cattle. Considering 67 animals, DNA sequencing of the genes’ exons, flanking regions and parts of the 5′-upstream regions identified 1058 genetic variants including 731 previously unknown. In addition, four novel milk protein variants were detected, including <i>CSN3<sup>K</sup></i> (p.Ala66Val), <i>LALBA<sup>F</sup></i> (p.Arg58Trp), <i>LGB<sup>B1</sup></i> (p.Ala134Val) and <i>LGB<sup>K</sup></i> (p.Thr92Asnfs*13). <i>CSN3<sup>K</sup></i> is caused by a novel SNP (BTA6:85656526C>T, exon 4) whereas <i>LALBA<sup>F</sup></i> and <i>LGB<sup>B1</sup></i> are due to rs714688595C>T (exon 1) and rs109625649C>T (exon 4), respectively. Regarding <i>LGB<sup>K</sup></i>, a frameshift insertion of one adenine residue at BTA11:103257980 (exon 3) induces a premature translation termination resulting in a 46% reduction of the reference protein sequence. The casein polymorphisms formed five main <i>CSN1S1-CSN2-CSN1S2-CSN3</i> haplotypes including B-A1-A-B, B-A1-A-A and C-A2-A-B which are predominant in the investigated cattle breeds. Moreover, in silico analyses of polymorphisms within the 5′- and 3′- untranslated regions of all six milk proteins revealed effects on microRNA and transcription factor binding sites. This study suggests a large genetic variation of milk protein genes in Beninese cattle, which should be investigated in further studies for their effects on milk production, including quality and yield traits. |
| first_indexed | 2024-03-10T05:28:36Z |
| format | Article |
| id | doaj.art-77f068f4ca784cecac8c86ba4da9c466 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T05:28:36Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-77f068f4ca784cecac8c86ba4da9c4662023-11-22T23:27:32ZengMDPI AGGenes2073-44252021-10-011211170210.3390/genes12111702First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein VariantsSèyi Fridaïus Ulrich Vanvanhossou0Isabella Jasmin Giambra1Tong Yin2Kerstin Brügemann3Luc Hippolyte Dossa4Sven König5Institute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, 35390 Gießen, GermanyInstitute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, 35390 Gießen, GermanyInstitute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, 35390 Gießen, GermanyInstitute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, 35390 Gießen, GermanySchool of Science and Technics of Animal Production, Faculty of Agricultural Sciences, University of Abomey-Calavi, Abomey-Calavi, 03 BP 2819 Jéricho Cotonou, BeninInstitute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, 35390 Gießen, GermanyThis study investigated polymorphisms in the milk protein genes <i>CSN1S1</i>, <i>CSN2</i>, <i>CSN1S2</i>, <i>CSN3</i>, <i>LALBA</i>, and <i>LGB</i>, and casein haplotypes in Beninese indigenous cattle. Considering 67 animals, DNA sequencing of the genes’ exons, flanking regions and parts of the 5′-upstream regions identified 1058 genetic variants including 731 previously unknown. In addition, four novel milk protein variants were detected, including <i>CSN3<sup>K</sup></i> (p.Ala66Val), <i>LALBA<sup>F</sup></i> (p.Arg58Trp), <i>LGB<sup>B1</sup></i> (p.Ala134Val) and <i>LGB<sup>K</sup></i> (p.Thr92Asnfs*13). <i>CSN3<sup>K</sup></i> is caused by a novel SNP (BTA6:85656526C>T, exon 4) whereas <i>LALBA<sup>F</sup></i> and <i>LGB<sup>B1</sup></i> are due to rs714688595C>T (exon 1) and rs109625649C>T (exon 4), respectively. Regarding <i>LGB<sup>K</sup></i>, a frameshift insertion of one adenine residue at BTA11:103257980 (exon 3) induces a premature translation termination resulting in a 46% reduction of the reference protein sequence. The casein polymorphisms formed five main <i>CSN1S1-CSN2-CSN1S2-CSN3</i> haplotypes including B-A1-A-B, B-A1-A-A and C-A2-A-B which are predominant in the investigated cattle breeds. Moreover, in silico analyses of polymorphisms within the 5′- and 3′- untranslated regions of all six milk proteins revealed effects on microRNA and transcription factor binding sites. This study suggests a large genetic variation of milk protein genes in Beninese cattle, which should be investigated in further studies for their effects on milk production, including quality and yield traits.https://www.mdpi.com/2073-4425/12/11/1702casein and whey protein genesgenetic variantscasein haplotypeAfrican taurine and indicine breedsmicroRNAtranscription factor binding sites |
| spellingShingle | Sèyi Fridaïus Ulrich Vanvanhossou Isabella Jasmin Giambra Tong Yin Kerstin Brügemann Luc Hippolyte Dossa Sven König First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants Genes casein and whey protein genes genetic variants casein haplotype African taurine and indicine breeds microRNA transcription factor binding sites |
| title | First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants |
| title_full | First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants |
| title_fullStr | First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants |
| title_full_unstemmed | First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants |
| title_short | First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants |
| title_sort | first dna sequencing in beninese indigenous cattle breeds captures new milk protein variants |
| topic | casein and whey protein genes genetic variants casein haplotype African taurine and indicine breeds microRNA transcription factor binding sites |
| url | https://www.mdpi.com/2073-4425/12/11/1702 |
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