A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows
Cost-effective automated solutions for hydrogel-based 3D cell culture workflows are required to increase throughput, reproducibility and user-independent results. 3D bioprinters can handle viscous biomaterials, however, throughput is low, they are limited to specific biomaterials, and lack post-proc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137023000699 |
| _version_ | 1797733897453174784 |
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| author | Melanie Kahl Dominik Schneidereit Christoph Meinert Nathalie Bock Dietmar W. Hutmacher Oliver Friedrich |
| author_facet | Melanie Kahl Dominik Schneidereit Christoph Meinert Nathalie Bock Dietmar W. Hutmacher Oliver Friedrich |
| author_sort | Melanie Kahl |
| collection | DOAJ |
| description | Cost-effective automated solutions for hydrogel-based 3D cell culture workflows are required to increase throughput, reproducibility and user-independent results. 3D bioprinters can handle viscous biomaterials, however, throughput is low, they are limited to specific biomaterials, and lack post-processing and analysis methods. Conversely, liquid handling robots have higher throughput but miss viscous materials handling capabilities. Furthermore, commercial systems are not only expensive but mostly not ‘open-source’ and hence are not adaptable to specific experimental requirements.The aim of this study was the implementation of an opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows from production to analysis into a previously developed biomanufacturing workstation. An analysis module with a fluorescence wide-field microscope and a motorised XYZ stage was engineered to transport tissue-culture plates from the storage rack of the workstation to the custom-made microscope and to assess fluorescence-based cell parameters. The microscope has two fluorescence channels and a resolution greater than 228 lp/mm. The XYZ-stage achieved an accuracy of 0.8 μm in X-direction (repeatability 2.4 μm) and 1.4 μm in Z-direction (repeatability <0.1 μm). Viability assays and drug-response studies were conducted on 2D and 3D prostate cancer gelatin-methacryloyl hydrogel models demonstrating its capabilities comparable to a conventional fluorescence microscope. Altogether, the integration of an opto-mechatronic screening module into the biomanufacturing workstation enables automated, reproducible, and user-independent production, imaging and analysis of hydrogel-based 3D cell cultures. |
| first_indexed | 2024-03-12T12:35:17Z |
| format | Article |
| id | doaj.art-77f357cb19f348b89be4ceb3628af8e2 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-03-12T12:35:17Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-77f357cb19f348b89be4ceb3628af8e22023-08-29T04:17:55ZengElsevierBiosensors and Bioelectronics: X2590-13702023-09-0114100372A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflowsMelanie Kahl0Dominik Schneidereit1Christoph Meinert2Nathalie Bock3Dietmar W. Hutmacher4Oliver Friedrich5Institute of Medical Biotechnology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany; School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; Centre for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia; Corresponding author. Institute of Medical Biotechnology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.Institute of Medical Biotechnology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, GermanyCentre for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia; Herston Biofabrication Institute, Metro North Hospital and Health Services, Brisbane, Queensland, AustraliaSchool of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; Centre for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia; Translational Research Institute, Queensland University of Technology, Woolloongabba, 4102, QLD, AustraliaSchool of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland, Australia; ARC ITTC in Additive Biomanufacturing, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, AustraliaInstitute of Medical Biotechnology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany; School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland, AustraliaCost-effective automated solutions for hydrogel-based 3D cell culture workflows are required to increase throughput, reproducibility and user-independent results. 3D bioprinters can handle viscous biomaterials, however, throughput is low, they are limited to specific biomaterials, and lack post-processing and analysis methods. Conversely, liquid handling robots have higher throughput but miss viscous materials handling capabilities. Furthermore, commercial systems are not only expensive but mostly not ‘open-source’ and hence are not adaptable to specific experimental requirements.The aim of this study was the implementation of an opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows from production to analysis into a previously developed biomanufacturing workstation. An analysis module with a fluorescence wide-field microscope and a motorised XYZ stage was engineered to transport tissue-culture plates from the storage rack of the workstation to the custom-made microscope and to assess fluorescence-based cell parameters. The microscope has two fluorescence channels and a resolution greater than 228 lp/mm. The XYZ-stage achieved an accuracy of 0.8 μm in X-direction (repeatability 2.4 μm) and 1.4 μm in Z-direction (repeatability <0.1 μm). Viability assays and drug-response studies were conducted on 2D and 3D prostate cancer gelatin-methacryloyl hydrogel models demonstrating its capabilities comparable to a conventional fluorescence microscope. Altogether, the integration of an opto-mechatronic screening module into the biomanufacturing workstation enables automated, reproducible, and user-independent production, imaging and analysis of hydrogel-based 3D cell cultures.http://www.sciencedirect.com/science/article/pii/S2590137023000699Open-sourceAutomationOpto-mechatronicFluorescence microscopeHigh-throughputHydrogel-based 3D culture models |
| spellingShingle | Melanie Kahl Dominik Schneidereit Christoph Meinert Nathalie Bock Dietmar W. Hutmacher Oliver Friedrich A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows Biosensors and Bioelectronics: X Open-source Automation Opto-mechatronic Fluorescence microscope High-throughput Hydrogel-based 3D culture models |
| title | A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows |
| title_full | A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows |
| title_fullStr | A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows |
| title_full_unstemmed | A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows |
| title_short | A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows |
| title_sort | fluorescence based opto mechatronic screening module omsm for automated 3d cell culture workflows |
| topic | Open-source Automation Opto-mechatronic Fluorescence microscope High-throughput Hydrogel-based 3D culture models |
| url | http://www.sciencedirect.com/science/article/pii/S2590137023000699 |
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