Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress
Coptisine is the major bioactive protoberberine alkaloid found in <i>Rhizoma Coptidis.</i> Coptisine reduces inflammatory responses and improves glucose tolerance; nevertheless, whether coptisine has vasoprotective effect in diabetes is not fully characterized. Conduit arteries including...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/1420-3049/26/14/4210 |
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| author | Yan Zhou Chunxiu Zhou Xutao Zhang Chi Teng Vong Yitao Wang Wai San Cheang |
| author_facet | Yan Zhou Chunxiu Zhou Xutao Zhang Chi Teng Vong Yitao Wang Wai San Cheang |
| author_sort | Yan Zhou |
| collection | DOAJ |
| description | Coptisine is the major bioactive protoberberine alkaloid found in <i>Rhizoma Coptidis.</i> Coptisine reduces inflammatory responses and improves glucose tolerance; nevertheless, whether coptisine has vasoprotective effect in diabetes is not fully characterized. Conduit arteries including aortas and carotid arteries were obtained from male C57BL/6J mice for <i>ex vivo</i> treatment with risk factors (high glucose or tunicamycin) and coptisine. Some arterial rings were obtained from diabetic mice, which were induced by high-fat diet (45% kcal% fat) feeding for 6 weeks combined with a low-dose intraperitoneal injection of streptozotocin (120 mg/kg). Functional studies showed that coptisine protected endothelium-dependent relaxation in aortas against risk factors and from diabetic mice. Coptisine increased phosphorylations of AMPK and eNOS and downregulated the endoplasmic reticulum (ER) stress markers as determined by Western blotting. Coptisine elevates NO bioavailability and decreases reactive oxygen species level. The results indicate that coptisine improves vascular function in diabetes through suppression of ER stress and oxidative stress, implying the therapeutic potential of coptisine to treat diabetic vasculopathy. |
| first_indexed | 2024-03-10T09:30:35Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:30:35Z |
| publishDate | 2021-07-01 |
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| spelling | doaj.art-77f37b1a70be47658576a234f5e3b6252023-11-22T04:30:16ZengMDPI AGMolecules1420-30492021-07-012614421010.3390/molecules26144210Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative StressYan Zhou0Chunxiu Zhou1Xutao Zhang2Chi Teng Vong3Yitao Wang4Wai San Cheang5State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, ChinaCoptisine is the major bioactive protoberberine alkaloid found in <i>Rhizoma Coptidis.</i> Coptisine reduces inflammatory responses and improves glucose tolerance; nevertheless, whether coptisine has vasoprotective effect in diabetes is not fully characterized. Conduit arteries including aortas and carotid arteries were obtained from male C57BL/6J mice for <i>ex vivo</i> treatment with risk factors (high glucose or tunicamycin) and coptisine. Some arterial rings were obtained from diabetic mice, which were induced by high-fat diet (45% kcal% fat) feeding for 6 weeks combined with a low-dose intraperitoneal injection of streptozotocin (120 mg/kg). Functional studies showed that coptisine protected endothelium-dependent relaxation in aortas against risk factors and from diabetic mice. Coptisine increased phosphorylations of AMPK and eNOS and downregulated the endoplasmic reticulum (ER) stress markers as determined by Western blotting. Coptisine elevates NO bioavailability and decreases reactive oxygen species level. The results indicate that coptisine improves vascular function in diabetes through suppression of ER stress and oxidative stress, implying the therapeutic potential of coptisine to treat diabetic vasculopathy.https://www.mdpi.com/1420-3049/26/14/4210coptisinediabetesendoplasmic reticulum stressendothelial dysfunctionoxidative stress |
| spellingShingle | Yan Zhou Chunxiu Zhou Xutao Zhang Chi Teng Vong Yitao Wang Wai San Cheang Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress Molecules coptisine diabetes endoplasmic reticulum stress endothelial dysfunction oxidative stress |
| title | Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress |
| title_full | Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress |
| title_fullStr | Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress |
| title_full_unstemmed | Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress |
| title_short | Coptisine Attenuates Diabetes—Associated Endothelial Dysfunction through Inhibition of Endoplasmic Reticulum Stress and Oxidative Stress |
| title_sort | coptisine attenuates diabetes associated endothelial dysfunction through inhibition of endoplasmic reticulum stress and oxidative stress |
| topic | coptisine diabetes endoplasmic reticulum stress endothelial dysfunction oxidative stress |
| url | https://www.mdpi.com/1420-3049/26/14/4210 |
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