Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy
Potato is an important food crop. After harvest, these tubers will undergo a period of dormancy. Brassinosteroids (BRs) are a new class of plant hormones that regulate plant growth and seed germination. In this study, 500 nM of BR was able to break the dormancy of tubers. Additionally, exogenous BR...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/4/2244 |
| Summary: | Potato is an important food crop. After harvest, these tubers will undergo a period of dormancy. Brassinosteroids (BRs) are a new class of plant hormones that regulate plant growth and seed germination. In this study, 500 nM of BR was able to break the dormancy of tubers. Additionally, exogenous BR also upregulated BR signal transduction genes, except for <i>StBIN2</i>. <i>StBIN2</i> is a negative regulator of BR, but its specific role in tuber dormancy remains unclear. Transgenic methods were used to regulate the expression level of <i>StBIN2</i> in tubers. It was demonstrated that the overexpression of <i>StBIN2</i> significantly prolonged tuber dormancy while silencing <i>StBIN2</i> led to premature sprouting. To further investigate the effect of <i>StBIN2</i> on tuber dormancy, RNA-Seq was used to analyze the differentially expressed genes in <i>OE</i>-<i>StBIN2</i>, <i>RNAi</i>-<i>StBIN2</i>, and WT tubers. The results showed that <i>StBIN2</i> upregulated the expression of ABA signal transduction genes but inhibited the expression of lignin synthesis key genes. Meanwhile, it was also found that StBIN2 physically interacted with StSnRK2.2 and StCCJ9. These results indicate that <i>StBIN2</i> maintains tuber dormancy by mediating ABA signal transduction and lignin synthesis. The findings of this study will help us better understand the molecular mechanisms underlying potato tuber dormancy and provide theoretical support for the development of new varieties using related genes. |
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| ISSN: | 1661-6596 1422-0067 |