Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation
Wound healing is a complex process that requires specific interactions between multiple cells such as fibroblasts, mesenchymal, endothelial, and neural stem cells. Recent studies have shown that calcium silicate (CS)-based biomaterials can enhance the secretion of growth factors from fibroblasts, wh...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/1/70 |
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| author | Fong-Sian Lin Jian-Jr Lee Alvin Kai-Xing Lee Chia-Che Ho Yen-Ting Liu Ming-You Shie |
| author_facet | Fong-Sian Lin Jian-Jr Lee Alvin Kai-Xing Lee Chia-Che Ho Yen-Ting Liu Ming-You Shie |
| author_sort | Fong-Sian Lin |
| collection | DOAJ |
| description | Wound healing is a complex process that requires specific interactions between multiple cells such as fibroblasts, mesenchymal, endothelial, and neural stem cells. Recent studies have shown that calcium silicate (CS)-based biomaterials can enhance the secretion of growth factors from fibroblasts, which further increased wound healing and skin regeneration. In addition, gelatin methacrylate (GelMa) is a compatible biomaterial that is commonly used in tissue engineering. However, it has low mechanical properties, thus restricting its fullest potential for clinical applications. In this study, we infused Si ions into GelMa hydrogel and assessed for its feasibility for skin regeneration applications by observing for its influences on human dermal fibroblasts (hDF). Initial studies showed that Si could be successfully incorporated into GelMa, and printability was not affected. The degradability of Si-GelMa was approximately 20% slower than GelMa hydrogels, thus allowing for better wound healing and regeneration. Furthermore, Si-GelMa enhanced cellular adhesion and proliferation, therefore leading to the increased secretion of collagen I other important extracellular matrix (ECM) remodeling-related proteins including Ki67, MMP9, and decorin. This study showed that the Si-GelMa hydrogels were able to enhance the activity of hDF due to the gradual release of Si ions, thus making it a potential candidate for future skin regeneration clinical applications. |
| first_indexed | 2024-03-10T13:44:36Z |
| format | Article |
| id | doaj.art-77f86b4d10704b34a22d2cb91e923dfc |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T13:44:36Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-77f86b4d10704b34a22d2cb91e923dfc2023-11-21T02:42:06ZengMDPI AGPolymers2073-43602020-12-011317010.3390/polym13010070Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and DifferentiationFong-Sian Lin0Jian-Jr Lee1Alvin Kai-Xing Lee2Chia-Che Ho3Yen-Ting Liu4Ming-You Shie5x-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung City 40447, TaiwanSchool of Medicine, China Medical University, Taichung City 40447, Taiwanx-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung City 40447, TaiwanDepartment of Bioinformatics and Medical Engineering, Asia University, Taichung City 41354, Taiwanx-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung City 40447, Taiwanx-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung City 40447, TaiwanWound healing is a complex process that requires specific interactions between multiple cells such as fibroblasts, mesenchymal, endothelial, and neural stem cells. Recent studies have shown that calcium silicate (CS)-based biomaterials can enhance the secretion of growth factors from fibroblasts, which further increased wound healing and skin regeneration. In addition, gelatin methacrylate (GelMa) is a compatible biomaterial that is commonly used in tissue engineering. However, it has low mechanical properties, thus restricting its fullest potential for clinical applications. In this study, we infused Si ions into GelMa hydrogel and assessed for its feasibility for skin regeneration applications by observing for its influences on human dermal fibroblasts (hDF). Initial studies showed that Si could be successfully incorporated into GelMa, and printability was not affected. The degradability of Si-GelMa was approximately 20% slower than GelMa hydrogels, thus allowing for better wound healing and regeneration. Furthermore, Si-GelMa enhanced cellular adhesion and proliferation, therefore leading to the increased secretion of collagen I other important extracellular matrix (ECM) remodeling-related proteins including Ki67, MMP9, and decorin. This study showed that the Si-GelMa hydrogels were able to enhance the activity of hDF due to the gradual release of Si ions, thus making it a potential candidate for future skin regeneration clinical applications.https://www.mdpi.com/2073-4360/13/1/70gelatin–methacryloylfibroblasthydrogelcalcium silicateextracellular matrix remodeling |
| spellingShingle | Fong-Sian Lin Jian-Jr Lee Alvin Kai-Xing Lee Chia-Che Ho Yen-Ting Liu Ming-You Shie Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation Polymers gelatin–methacryloyl fibroblast hydrogel calcium silicate extracellular matrix remodeling |
| title | Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation |
| title_full | Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation |
| title_fullStr | Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation |
| title_full_unstemmed | Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation |
| title_short | Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation |
| title_sort | calcium silicate activated gelatin methacrylate hydrogel for accelerating human dermal fibroblast proliferation and differentiation |
| topic | gelatin–methacryloyl fibroblast hydrogel calcium silicate extracellular matrix remodeling |
| url | https://www.mdpi.com/2073-4360/13/1/70 |
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