Characterization of circulating natural killer cells in neotropical primates.
Despite extensive use of nonhuman primates as models for infectious diseases and reproductive biology, imprecise phenotypic and functional definitions exist for natural killer (NK) cells. This deficit is particularly significant in the burgeoning use of small, less expensive New World primate specie...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3823947?pdf=render |
| _version_ | 1818127882529013760 |
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| author | Angela Carville Tristan I Evans R Keith Reeves |
| author_facet | Angela Carville Tristan I Evans R Keith Reeves |
| author_sort | Angela Carville |
| collection | DOAJ |
| description | Despite extensive use of nonhuman primates as models for infectious diseases and reproductive biology, imprecise phenotypic and functional definitions exist for natural killer (NK) cells. This deficit is particularly significant in the burgeoning use of small, less expensive New World primate species. Using polychromatic flow cytometry, we identified peripheral blood NK cells as CD3-negative and expressing a cluster of cell surface molecules characteristic of NK cells (i.e., NKG2A, NKp46, NKp30) in three New World primate species - common marmosets, cotton-top tamarins, and squirrel monkeys. We then assessed subset distribution using the classical NK markers, CD56 and CD16. In all species, similar to Old World primates, only a minor subset of NK cells was CD56+, and the dominant subset was CD56-CD16+. Interestingly, CD56+ NK cells were primarily cytokine-secreting cells, whereas CD56-CD16+ NK cells expressed significantly greater levels of intracellular perforin, suggesting these cells might have greater potential for cytotoxicity. New World primate species, like Old World primates, also had a minor CD56-CD16- NK cell subset that has no obvious counterpart in humans. Herein we present phenotypic profiles of New World primate NK cell subpopulations that are generally analogous to those found in humans. This conservation among species should support the further use of these species for biomedical research. |
| first_indexed | 2024-12-11T07:24:25Z |
| format | Article |
| id | doaj.art-77feb5b9e3d9447d874be7a64e0b4fa0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T07:24:25Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-77feb5b9e3d9447d874be7a64e0b4fa02022-12-22T01:15:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7879310.1371/journal.pone.0078793Characterization of circulating natural killer cells in neotropical primates.Angela CarvilleTristan I EvansR Keith ReevesDespite extensive use of nonhuman primates as models for infectious diseases and reproductive biology, imprecise phenotypic and functional definitions exist for natural killer (NK) cells. This deficit is particularly significant in the burgeoning use of small, less expensive New World primate species. Using polychromatic flow cytometry, we identified peripheral blood NK cells as CD3-negative and expressing a cluster of cell surface molecules characteristic of NK cells (i.e., NKG2A, NKp46, NKp30) in three New World primate species - common marmosets, cotton-top tamarins, and squirrel monkeys. We then assessed subset distribution using the classical NK markers, CD56 and CD16. In all species, similar to Old World primates, only a minor subset of NK cells was CD56+, and the dominant subset was CD56-CD16+. Interestingly, CD56+ NK cells were primarily cytokine-secreting cells, whereas CD56-CD16+ NK cells expressed significantly greater levels of intracellular perforin, suggesting these cells might have greater potential for cytotoxicity. New World primate species, like Old World primates, also had a minor CD56-CD16- NK cell subset that has no obvious counterpart in humans. Herein we present phenotypic profiles of New World primate NK cell subpopulations that are generally analogous to those found in humans. This conservation among species should support the further use of these species for biomedical research.http://europepmc.org/articles/PMC3823947?pdf=render |
| spellingShingle | Angela Carville Tristan I Evans R Keith Reeves Characterization of circulating natural killer cells in neotropical primates. PLoS ONE |
| title | Characterization of circulating natural killer cells in neotropical primates. |
| title_full | Characterization of circulating natural killer cells in neotropical primates. |
| title_fullStr | Characterization of circulating natural killer cells in neotropical primates. |
| title_full_unstemmed | Characterization of circulating natural killer cells in neotropical primates. |
| title_short | Characterization of circulating natural killer cells in neotropical primates. |
| title_sort | characterization of circulating natural killer cells in neotropical primates |
| url | http://europepmc.org/articles/PMC3823947?pdf=render |
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