Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis.
Clonorchis sinensis is the causative agent of the life-threatening disease endemic to China, Korea, and Vietnam. It is estimated that about 15 million people are infected with this fluke. C. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause...
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| Formatua: | Artikulua |
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Public Library of Science (PLoS)
2011-06-01
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| Saila: | PLoS Neglected Tropical Diseases |
| Sarrera elektronikoa: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001208&type=printable |
| _version_ | 1826800202334339072 |
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| author | Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong |
| author_facet | Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong |
| author_sort | Won Gi Yoo |
| collection | DOAJ |
| description | Clonorchis sinensis is the causative agent of the life-threatening disease endemic to China, Korea, and Vietnam. It is estimated that about 15 million people are infected with this fluke. C. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause cholangiocarcinoma in chronically infected individuals. Accumulation of a large amount of biological information about the adult stage of this liver fluke in recent years has advanced our understanding of the pathological interplay between this parasite and its hosts. However, no developmental gene expression profiles of C. sinensis have been published. In this study, we generated gene expression profiles of three developmental stages of C. sinensis by analyzing expressed sequence tags (ESTs). Complementary DNA libraries were constructed from the adult, metacercaria, and egg developmental stages of C. sinensis. A total of 52,745 ESTs were generated and assembled into 12,830 C. sinensis assembled EST sequences, and then these assemblies were further categorized into groups according to biological functions and developmental stages. Most of the genes that were differentially expressed in the different stages were consistent with the biological and physical features of the particular developmental stage; high energy metabolism, motility and reproduction genes were differentially expressed in adults, minimal metabolism and final host adaptation genes were differentially expressed in metacercariae, and embryonic genes were differentially expressed in eggs. The higher expression of glucose transporters, proteases, and antioxidant enzymes in the adults accounts for active uptake of nutrients and defense against host immune attacks. The types of ion channels present in C. sinensis are consistent with its parasitic nature and phylogenetic placement in the tree of life. We anticipate that the transcriptomic information on essential regulators of development, bile chemotaxis, and physico-metabolic pathways in C. sinensis that presented in this study will guide further studies to identify novel drug targets and diagnostic antigens. |
| first_indexed | 2024-12-12T19:11:17Z |
| format | Article |
| id | doaj.art-77ffebc058104d17b9401f7fb8c95ae3 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2025-03-17T00:47:14Z |
| publishDate | 2011-06-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-77ffebc058104d17b9401f7fb8c95ae32025-02-20T05:31:51ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352011-06-0156e120810.1371/journal.pntd.0001208Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis.Won Gi YooDae-Won KimJung-Won JuPyo Yun ChoTae Im KimShin-Hyeong ChoSang-Haeng ChoiHong-Seog ParkTong-Soo KimSung-Jong HongClonorchis sinensis is the causative agent of the life-threatening disease endemic to China, Korea, and Vietnam. It is estimated that about 15 million people are infected with this fluke. C. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause cholangiocarcinoma in chronically infected individuals. Accumulation of a large amount of biological information about the adult stage of this liver fluke in recent years has advanced our understanding of the pathological interplay between this parasite and its hosts. However, no developmental gene expression profiles of C. sinensis have been published. In this study, we generated gene expression profiles of three developmental stages of C. sinensis by analyzing expressed sequence tags (ESTs). Complementary DNA libraries were constructed from the adult, metacercaria, and egg developmental stages of C. sinensis. A total of 52,745 ESTs were generated and assembled into 12,830 C. sinensis assembled EST sequences, and then these assemblies were further categorized into groups according to biological functions and developmental stages. Most of the genes that were differentially expressed in the different stages were consistent with the biological and physical features of the particular developmental stage; high energy metabolism, motility and reproduction genes were differentially expressed in adults, minimal metabolism and final host adaptation genes were differentially expressed in metacercariae, and embryonic genes were differentially expressed in eggs. The higher expression of glucose transporters, proteases, and antioxidant enzymes in the adults accounts for active uptake of nutrients and defense against host immune attacks. The types of ion channels present in C. sinensis are consistent with its parasitic nature and phylogenetic placement in the tree of life. We anticipate that the transcriptomic information on essential regulators of development, bile chemotaxis, and physico-metabolic pathways in C. sinensis that presented in this study will guide further studies to identify novel drug targets and diagnostic antigens.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001208&type=printable |
| spellingShingle | Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. PLoS Neglected Tropical Diseases |
| title | Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
| title_full | Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
| title_fullStr | Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
| title_full_unstemmed | Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
| title_short | Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
| title_sort | developmental transcriptomic features of the carcinogenic liver fluke clonorchis sinensis |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001208&type=printable |
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