Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus
Summary: Investigating flowing red blood cell (RBC) morphology and orientation is important for elucidating physiology and disease; existing commercially available products are limited to observing cell populations or single cells. In this protocol, we create a custom apparatus that combines coaxial...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722001599 |
| _version_ | 1818668187510636544 |
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| author | Tom Fitzpatrick Michael J. Simmonds Antony P. McNamee |
| author_facet | Tom Fitzpatrick Michael J. Simmonds Antony P. McNamee |
| author_sort | Tom Fitzpatrick |
| collection | DOAJ |
| description | Summary: Investigating flowing red blood cell (RBC) morphology and orientation is important for elucidating physiology and disease; existing commercially available products are limited to observing cell populations or single cells. In this protocol, we create a custom apparatus that combines coaxial brightfield microscopy with laser diffractometry to inspect near-real-time deformability, morphology, and orientation of flowing RBCs. There are difficulties associated with building optical systems for biological inspection; however, this protocol provides a suitable framework for developing an ''ektacytoscope'' for studying blood cells.For complete details on the use and execution of this protocol, please refer to McNamee et al. (2020). |
| first_indexed | 2024-12-17T06:32:20Z |
| format | Article |
| id | doaj.art-7800d13ec0344ac6bc0cdcc266c42080 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T06:32:20Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-7800d13ec0344ac6bc0cdcc266c420802022-12-21T22:00:07ZengElsevierSTAR Protocols2666-16672022-06-0132101279Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatusTom Fitzpatrick0Michael J. Simmonds1Antony P. McNamee2School of Environment and Science, Griffith University, Gold Coast, QLD 4222, Australia; Corresponding authorBiorheology Research Laboratory, Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD 4222, AustraliaBiorheology Research Laboratory, Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD 4222, Australia; Corresponding authorSummary: Investigating flowing red blood cell (RBC) morphology and orientation is important for elucidating physiology and disease; existing commercially available products are limited to observing cell populations or single cells. In this protocol, we create a custom apparatus that combines coaxial brightfield microscopy with laser diffractometry to inspect near-real-time deformability, morphology, and orientation of flowing RBCs. There are difficulties associated with building optical systems for biological inspection; however, this protocol provides a suitable framework for developing an ''ektacytoscope'' for studying blood cells.For complete details on the use and execution of this protocol, please refer to McNamee et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166722001599BiophysicsCell BiologyCell MembraneMicroscopySystems biologyBiotechnology and bioengineering |
| spellingShingle | Tom Fitzpatrick Michael J. Simmonds Antony P. McNamee Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus STAR Protocols Biophysics Cell Biology Cell Membrane Microscopy Systems biology Biotechnology and bioengineering |
| title | Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus |
| title_full | Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus |
| title_fullStr | Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus |
| title_full_unstemmed | Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus |
| title_short | Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus |
| title_sort | protocol for inspecting blood cell dynamics with a custom ektacytometer rheometer apparatus |
| topic | Biophysics Cell Biology Cell Membrane Microscopy Systems biology Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722001599 |
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