Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice
Aim: In this study, Staphylococcus phage JD007 bactericidal activity and induced immune responses during treatment were assessed in a dermal abscess model.Materials and Methods: Dermal abscesses in nude mice were established by injecting a clinical isolate of S. aureus SA325 isolated from the back u...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01553/full |
| _version_ | 1828268468585103360 |
|---|---|
| author | Bingyu Ding Bingyu Ding Qingtian Li Mingquan Guo Ke Dong Yan Zhang Xiaokui Guo Qingzhong Liu Li Li Zelin Cui |
| author_facet | Bingyu Ding Bingyu Ding Qingtian Li Mingquan Guo Ke Dong Yan Zhang Xiaokui Guo Qingzhong Liu Li Li Zelin Cui |
| author_sort | Bingyu Ding |
| collection | DOAJ |
| description | Aim: In this study, Staphylococcus phage JD007 bactericidal activity and induced immune responses during treatment were assessed in a dermal abscess model.Materials and Methods: Dermal abscesses in nude mice were established by injecting a clinical isolate of S. aureus SA325 isolated from the back under-dermal abscess of an in-patient.Results: Phage JD007 was able to inhibit the growth of S. aureus SA325 at MOI = 1 or 10, significantly preventing the formation of dermal abscesses. Moderate immune responses were observed in the prevention group through detection of cytokines.Conclusion: Phage JD007 inhibits the formation of dermal abscesses caused by a clinical S. aureus strain in nude mice without robust immune responses. |
| first_indexed | 2024-04-13T05:19:19Z |
| format | Article |
| id | doaj.art-78011571ba5a4afcbb95fe036eeeed52 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T05:19:19Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-78011571ba5a4afcbb95fe036eeeed522022-12-22T03:00:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01553315482Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude MiceBingyu Ding0Bingyu Ding1Qingtian Li2Mingquan Guo3Ke Dong4Yan Zhang5Xiaokui Guo6Qingzhong Liu7Li Li8Zelin Cui9Department of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaAim: In this study, Staphylococcus phage JD007 bactericidal activity and induced immune responses during treatment were assessed in a dermal abscess model.Materials and Methods: Dermal abscesses in nude mice were established by injecting a clinical isolate of S. aureus SA325 isolated from the back under-dermal abscess of an in-patient.Results: Phage JD007 was able to inhibit the growth of S. aureus SA325 at MOI = 1 or 10, significantly preventing the formation of dermal abscesses. Moderate immune responses were observed in the prevention group through detection of cytokines.Conclusion: Phage JD007 inhibits the formation of dermal abscesses caused by a clinical S. aureus strain in nude mice without robust immune responses.https://www.frontiersin.org/article/10.3389/fmicb.2018.01553/fullStaphylococcus aureusdermal abscessinhibitionnude mousephage JD007 |
| spellingShingle | Bingyu Ding Bingyu Ding Qingtian Li Mingquan Guo Ke Dong Yan Zhang Xiaokui Guo Qingzhong Liu Li Li Zelin Cui Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice Frontiers in Microbiology Staphylococcus aureus dermal abscess inhibition nude mouse phage JD007 |
| title | Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice |
| title_full | Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice |
| title_fullStr | Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice |
| title_full_unstemmed | Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice |
| title_short | Prevention of Dermal Abscess Formation Caused by Staphylococcus aureus Using Phage JD007 in Nude Mice |
| title_sort | prevention of dermal abscess formation caused by staphylococcus aureus using phage jd007 in nude mice |
| topic | Staphylococcus aureus dermal abscess inhibition nude mouse phage JD007 |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01553/full |
| work_keys_str_mv | AT bingyuding preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT bingyuding preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT qingtianli preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT mingquanguo preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT kedong preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT yanzhang preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT xiaokuiguo preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT qingzhongliu preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT lili preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice AT zelincui preventionofdermalabscessformationcausedbystaphylococcusaureususingphagejd007innudemice |