Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.).
Nitric oxide is a gaseous signalling molecule and has a profound impact on plant growth and development. It is reported to serve as pro oxidant as well as antioxidant in plant system. In present study, we evaluated the protective role of nitric oxide against AsV toxicity in rice plants. Arsenate exp...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01272/full |
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| author | Amit Pal Singh Garima eDixit Amit eKumar Seema eMishra Pradyumna Kumar Singh Sanjay eDwivedi Prabodh Kumar Trivedi Debasis eChakrabarty Shekhar eMallick Vivek ePandey Om Parkash eDhankher Rudra Deo Tripathi |
| author_facet | Amit Pal Singh Garima eDixit Amit eKumar Seema eMishra Pradyumna Kumar Singh Sanjay eDwivedi Prabodh Kumar Trivedi Debasis eChakrabarty Shekhar eMallick Vivek ePandey Om Parkash eDhankher Rudra Deo Tripathi |
| author_sort | Amit Pal Singh |
| collection | DOAJ |
| description | Nitric oxide is a gaseous signalling molecule and has a profound impact on plant growth and development. It is reported to serve as pro oxidant as well as antioxidant in plant system. In present study, we evaluated the protective role of nitric oxide against AsV toxicity in rice plants. Arsenate exposure has hampered the plant growth, reduced the chlorophyll content and enhanced the oxidative stress while the exogenous NO supplementation has reverted these symptoms. Nitric oxide supplementation has reduced the As accumulation in root as well as shoot. Nitric oxide supplementation to AsV exposed plants has reduced the gene expression level of OsLsi1 and OsLsi2. Arsenate stress significantly impacted thiol metabolism, it reduced GSH content and GSH/GSSG ratio and enhanced the level of PCs. Nitric oxide supplementation maintained the GSH/GSSG ratio and reduced the level of PCs. Nitric oxide supplementation reverted AsV induced iron deficiency in shoot and had significant impact of gene expression level of various iron transporters (OsYSL2, OsFRDL1, OsIRT1 and OsIRO2). Conclusively, exogenous application of nitric oxide could be advantageous against AsV toxicity and could confer the tolerance to AsV stress in rice. |
| first_indexed | 2024-04-12T12:45:58Z |
| format | Article |
| id | doaj.art-78016671bd6a413894087dace9500f83 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T12:45:58Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-78016671bd6a413894087dace9500f832022-12-22T03:32:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-01-01610.3389/fpls.2015.01272172634Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.).Amit Pal Singh0Garima eDixit1Amit eKumar2Seema eMishra3Pradyumna Kumar Singh4Sanjay eDwivedi5Prabodh Kumar Trivedi6Debasis eChakrabarty7Shekhar eMallick8Vivek ePandey9Om Parkash eDhankher10Rudra Deo Tripathi11CSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteCSIR-National Botanical Research InstituteStockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003-9320, U.S.ACSIR-National Botanical Research InstituteNitric oxide is a gaseous signalling molecule and has a profound impact on plant growth and development. It is reported to serve as pro oxidant as well as antioxidant in plant system. In present study, we evaluated the protective role of nitric oxide against AsV toxicity in rice plants. Arsenate exposure has hampered the plant growth, reduced the chlorophyll content and enhanced the oxidative stress while the exogenous NO supplementation has reverted these symptoms. Nitric oxide supplementation has reduced the As accumulation in root as well as shoot. Nitric oxide supplementation to AsV exposed plants has reduced the gene expression level of OsLsi1 and OsLsi2. Arsenate stress significantly impacted thiol metabolism, it reduced GSH content and GSH/GSSG ratio and enhanced the level of PCs. Nitric oxide supplementation maintained the GSH/GSSG ratio and reduced the level of PCs. Nitric oxide supplementation reverted AsV induced iron deficiency in shoot and had significant impact of gene expression level of various iron transporters (OsYSL2, OsFRDL1, OsIRT1 and OsIRO2). Conclusively, exogenous application of nitric oxide could be advantageous against AsV toxicity and could confer the tolerance to AsV stress in rice.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01272/fullNitric Oxidearsenateiron transporterRice.Arsenic transporterNon protein thiol |
| spellingShingle | Amit Pal Singh Garima eDixit Amit eKumar Seema eMishra Pradyumna Kumar Singh Sanjay eDwivedi Prabodh Kumar Trivedi Debasis eChakrabarty Shekhar eMallick Vivek ePandey Om Parkash eDhankher Rudra Deo Tripathi Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.). Frontiers in Plant Science Nitric Oxide arsenate iron transporter Rice. Arsenic transporter Non protein thiol |
| title | Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.). |
| title_full | Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.). |
| title_fullStr | Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.). |
| title_full_unstemmed | Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.). |
| title_short | Nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice (Oryza sativa L.). |
| title_sort | nitric oxide alleviated arsenic toxicity by modulation of antioxidants and thiol metabolism in rice oryza sativa l |
| topic | Nitric Oxide arsenate iron transporter Rice. Arsenic transporter Non protein thiol |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01272/full |
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