Differentiation of Lactobacillus species by ARDRA
The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as s...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2013-04-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201302-0011_differentiation-of-lactobacillus-species-by-ardra.php |
| _version_ | 1797899682911879168 |
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| author | Kateřina Kšicová Marta Dušková Renáta Karpíšková |
| author_facet | Kateřina Kšicová Marta Dušková Renáta Karpíšková |
| author_sort | Kateřina Kšicová |
| collection | DOAJ |
| description | The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (AluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species. |
| first_indexed | 2024-04-10T08:33:47Z |
| format | Article |
| id | doaj.art-7801ef9a666d49e49bd3105009c7c38b |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:33:47Z |
| publishDate | 2013-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-7801ef9a666d49e49bd3105009c7c38b2023-02-23T03:27:59ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172013-04-0131218018810.17221/125/2012-CJFScjf-201302-0011Differentiation of Lactobacillus species by ARDRAKateřina Kšicová0Marta DuškováRenáta KarpíškováDepartment of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic, 2Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic, 3Veterinary Research Institute, Brno, Czech RepublicThe Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (AluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.https://cjfs.agriculturejournals.cz/artkey/cjf-201302-0011_differentiation-of-lactobacillus-species-by-ardra.phprestriction analysis of the 16s rdna genealuimspiin silico fragmentationspecies identification |
| spellingShingle | Kateřina Kšicová Marta Dušková Renáta Karpíšková Differentiation of Lactobacillus species by ARDRA Czech Journal of Food Sciences restriction analysis of the 16s rdna gene alui mspi in silico fragmentation species identification |
| title | Differentiation of Lactobacillus species by ARDRA |
| title_full | Differentiation of Lactobacillus species by ARDRA |
| title_fullStr | Differentiation of Lactobacillus species by ARDRA |
| title_full_unstemmed | Differentiation of Lactobacillus species by ARDRA |
| title_short | Differentiation of Lactobacillus species by ARDRA |
| title_sort | differentiation of lactobacillus species by ardra |
| topic | restriction analysis of the 16s rdna gene alui mspi in silico fragmentation species identification |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201302-0011_differentiation-of-lactobacillus-species-by-ardra.php |
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