Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium
Objective Orchastric changes in the mammary glands are vital, especially during lactation. The secretary epithelial cells together with the supporting myoepithelial and stromal cells function cordially to secrete milk. Increase in the number of luminal epithelial cells and a decrease in adipocytes a...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2020-02-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://ajas.info/upload/pdf/ajas-18-0806.pdf |
| _version_ | 1818286364111667200 |
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| author | Subi Sugathan Sung-Jin Lee Supriya Shiwani Naresh Kumar Singh |
| author_facet | Subi Sugathan Sung-Jin Lee Supriya Shiwani Naresh Kumar Singh |
| author_sort | Subi Sugathan |
| collection | DOAJ |
| description | Objective Orchastric changes in the mammary glands are vital, especially during lactation. The secretary epithelial cells together with the supporting myoepithelial and stromal cells function cordially to secrete milk. Increase in the number of luminal epithelial cells and a decrease in adipocytes are visible during lactation, whereas the reverse happens in the involution. However, an early involution occurs if the epithelial cells transdifferentiate towards adipocytes during the lactation period. We aimed to inhibit the adipocyte transdifferentiation of luminal cells by restraining the peroxisomal proliferator-activated receptor γ (PPARγ) pathway. Methods Linolenic acid (LA) and thiazolidinediones (TZDs) induced adipogenesis in mammary epithelial cells were conducted in monolayer, mixed culture as well as in transwell plate co-culture with mammary myoepithelial cells. Results Co-culture with myoepithelial cells showed higher adipogenic gene expression in epithelial cells under LA+TZDs treatment. Increase in the expressions of PPARγ, CCAAT/enhancer-binding protein α and vimentin in both mRNA as well as protein levels were observed. Whereas, bisphenol A diglycidyl ether treatment blocked LA+TZDs induced adipogenesis, as it could not show a significant rise in adipose related markers. Although comparative results were found in both mixed culture and monolayer conditions, co-culture technic was found to work better than the others. Conclusion Antagonizing PPARγ pathway in the presence of myoepithelial cells can significantly reduce the adipogenisis in epithelial cells, suggesting therapeutic inhibition of PPARγ can be considered to counter early involution or excessive adipogenesis in mammary epithelium in animals. |
| first_indexed | 2024-12-13T01:23:25Z |
| format | Article |
| id | doaj.art-78052b92870a4f6ba470ad0a76122034 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-13T01:23:25Z |
| publishDate | 2020-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-78052b92870a4f6ba470ad0a761220342022-12-22T00:04:11ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172020-02-0133234935910.5713/ajas.18.080624253Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepitheliumSubi Sugathan0Sung-Jin Lee1Supriya Shiwani2Naresh Kumar Singh3 Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea Department of Veterinary Surgery and Radiology, Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Banaras Hindu University,Varanasi-221005, Uttar Pradesh, IndiaObjective Orchastric changes in the mammary glands are vital, especially during lactation. The secretary epithelial cells together with the supporting myoepithelial and stromal cells function cordially to secrete milk. Increase in the number of luminal epithelial cells and a decrease in adipocytes are visible during lactation, whereas the reverse happens in the involution. However, an early involution occurs if the epithelial cells transdifferentiate towards adipocytes during the lactation period. We aimed to inhibit the adipocyte transdifferentiation of luminal cells by restraining the peroxisomal proliferator-activated receptor γ (PPARγ) pathway. Methods Linolenic acid (LA) and thiazolidinediones (TZDs) induced adipogenesis in mammary epithelial cells were conducted in monolayer, mixed culture as well as in transwell plate co-culture with mammary myoepithelial cells. Results Co-culture with myoepithelial cells showed higher adipogenic gene expression in epithelial cells under LA+TZDs treatment. Increase in the expressions of PPARγ, CCAAT/enhancer-binding protein α and vimentin in both mRNA as well as protein levels were observed. Whereas, bisphenol A diglycidyl ether treatment blocked LA+TZDs induced adipogenesis, as it could not show a significant rise in adipose related markers. Although comparative results were found in both mixed culture and monolayer conditions, co-culture technic was found to work better than the others. Conclusion Antagonizing PPARγ pathway in the presence of myoepithelial cells can significantly reduce the adipogenisis in epithelial cells, suggesting therapeutic inhibition of PPARγ can be considered to counter early involution or excessive adipogenesis in mammary epithelium in animals.http://ajas.info/upload/pdf/ajas-18-0806.pdftransdifferentiationperoxisomal proliferator-activated receptor γ (pparγ)linolenic acidluminal epitheliumadipogenesismyoepithelial |
| spellingShingle | Subi Sugathan Sung-Jin Lee Supriya Shiwani Naresh Kumar Singh Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium Asian-Australasian Journal of Animal Sciences transdifferentiation peroxisomal proliferator-activated receptor γ (pparγ) linolenic acid luminal epithelium adipogenesis myoepithelial |
| title | Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium |
| title_full | Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium |
| title_fullStr | Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium |
| title_full_unstemmed | Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium |
| title_short | Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium |
| title_sort | transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium |
| topic | transdifferentiation peroxisomal proliferator-activated receptor γ (pparγ) linolenic acid luminal epithelium adipogenesis myoepithelial |
| url | http://ajas.info/upload/pdf/ajas-18-0806.pdf |
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