Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>)
Fragrant woodfern (<i>Dryopteris fragrans</i>) is a medicinal plant rich in terpenoids. Ultraviolet-B (UV–B) light could increase concentration of terpenoids. The aim of this study was to analyze how UV–B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/10/5708 |
| _version_ | 1797499161884491776 |
|---|---|
| author | Chunhua Song Yalin Guan Dongrui Zhang Xun Tang Ying Chang |
| author_facet | Chunhua Song Yalin Guan Dongrui Zhang Xun Tang Ying Chang |
| author_sort | Chunhua Song |
| collection | DOAJ |
| description | Fragrant woodfern (<i>Dryopteris fragrans</i>) is a medicinal plant rich in terpenoids. Ultraviolet-B (UV–B) light could increase concentration of terpenoids. The aim of this study was to analyze how UV–B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant woodfern. In this study, compared with the control group, the content of the terpenes was significantly higher in fragrant woodfern leaves under UV–B treatment for 4 days (d). In order to identify how UV–B regulates the terpenoid metabolic mechanism in fragrant woodfern, we examined the mRNAs and small RNAs in fragrant woodfern leaves under UV–B treatment. mRNA and miRNA–seq identified 4533 DEGs and 17 DEMs in the control group compared with fragrant woodfern leaves under UV–B treatment for 4 d. mRNA–miRNA analysis identified miRNA target gene pairs consisting of 8 DEMs and 115 miRNAs. The target genes were subjected to GO and KEGG analyses. The results showed that the target genes were mainly enriched in diterpene biosynthesis, terpenoid backbone biosynthesis, plant hormone signal transduction, MEP pathway and MVA pathway, in which miR156 and miR160 regulate these pathways by targeting <i>DfSPL</i> and <i>DfARF</i>, respectively. The mRNA and miRNA datasets identified a subset of candidate genes. It provides the theoretical basis that UV–B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant woodfern. |
| first_indexed | 2024-03-10T03:43:29Z |
| format | Article |
| id | doaj.art-78056e314eb0446d9363418376de5082 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:43:29Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-78056e314eb0446d9363418376de50822023-11-23T11:27:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310570810.3390/ijms23105708Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>)Chunhua Song0Yalin Guan1Dongrui Zhang2Xun Tang3Ying Chang4College of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266100, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaFragrant woodfern (<i>Dryopteris fragrans</i>) is a medicinal plant rich in terpenoids. Ultraviolet-B (UV–B) light could increase concentration of terpenoids. The aim of this study was to analyze how UV–B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant woodfern. In this study, compared with the control group, the content of the terpenes was significantly higher in fragrant woodfern leaves under UV–B treatment for 4 days (d). In order to identify how UV–B regulates the terpenoid metabolic mechanism in fragrant woodfern, we examined the mRNAs and small RNAs in fragrant woodfern leaves under UV–B treatment. mRNA and miRNA–seq identified 4533 DEGs and 17 DEMs in the control group compared with fragrant woodfern leaves under UV–B treatment for 4 d. mRNA–miRNA analysis identified miRNA target gene pairs consisting of 8 DEMs and 115 miRNAs. The target genes were subjected to GO and KEGG analyses. The results showed that the target genes were mainly enriched in diterpene biosynthesis, terpenoid backbone biosynthesis, plant hormone signal transduction, MEP pathway and MVA pathway, in which miR156 and miR160 regulate these pathways by targeting <i>DfSPL</i> and <i>DfARF</i>, respectively. The mRNA and miRNA datasets identified a subset of candidate genes. It provides the theoretical basis that UV–B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant woodfern.https://www.mdpi.com/1422-0067/23/10/5708Fragrant woodfernterpenoid metabolic pathwaysUV–BmRNAsmall RNA |
| spellingShingle | Chunhua Song Yalin Guan Dongrui Zhang Xun Tang Ying Chang Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>) International Journal of Molecular Sciences Fragrant woodfern terpenoid metabolic pathways UV–B mRNA small RNA |
| title | Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>) |
| title_full | Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>) |
| title_fullStr | Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>) |
| title_full_unstemmed | Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>) |
| title_short | Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (<i>Dryopteris fragrans</i>) |
| title_sort | integrated mrna and mirna transcriptome analysis suggests a regulatory network for uv b controlled terpenoid synthesis in fragrant woodfern i dryopteris fragrans i |
| topic | Fragrant woodfern terpenoid metabolic pathways UV–B mRNA small RNA |
| url | https://www.mdpi.com/1422-0067/23/10/5708 |
| work_keys_str_mv | AT chunhuasong integratedmrnaandmirnatranscriptomeanalysissuggestsaregulatorynetworkforuvbcontrolledterpenoidsynthesisinfragrantwoodfernidryopterisfragransi AT yalinguan integratedmrnaandmirnatranscriptomeanalysissuggestsaregulatorynetworkforuvbcontrolledterpenoidsynthesisinfragrantwoodfernidryopterisfragransi AT dongruizhang integratedmrnaandmirnatranscriptomeanalysissuggestsaregulatorynetworkforuvbcontrolledterpenoidsynthesisinfragrantwoodfernidryopterisfragransi AT xuntang integratedmrnaandmirnatranscriptomeanalysissuggestsaregulatorynetworkforuvbcontrolledterpenoidsynthesisinfragrantwoodfernidryopterisfragransi AT yingchang integratedmrnaandmirnatranscriptomeanalysissuggestsaregulatorynetworkforuvbcontrolledterpenoidsynthesisinfragrantwoodfernidryopterisfragransi |