SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
Objective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia. Study Design: Cross-sectional comparative. Place and Duration of Study:...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Army Medical College Rawalpindi
2020-10-01
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| Series: | Pakistan Armed Forces Medical Journal |
| Subjects: | |
| Online Access: | https://www.pafmj.org/index.php/PAFMJ/article/view/5548/2951 |
| _version_ | 1830190167173890048 |
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| author | Munnaza Sarfraz Amir Rashid Midhat Mustafa Saleem Ahmad Khan Asifa Majeed |
| author_facet | Munnaza Sarfraz Amir Rashid Midhat Mustafa Saleem Ahmad Khan Asifa Majeed |
| author_sort | Munnaza Sarfraz |
| collection | DOAJ |
| description | Objective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia.
Study Design: Cross-sectional comparative.
Place and Duration of Study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi, from Jan to Dec 2017.
Methodology: Ninety subjects were selected and divided into three groups, thirty in each. The division was based on newly diagnosed patients of type 2 diabetes with dyslipidemia, newly diagnosed patients of type 2 diabetes and healthy individuals. Genomic DNA was extracted from the blood samples of all subjects. Exon-9 of the ABCA1 gene was amplified through polymerase chain reaction and sequenced on automated DNA sequencer. The biochemical data was analyzed by SPSS-20 and presented in percentage and mean SD.
Results: The pattern of sequences of ABCA1 gene was found normal in patients of the group I and II through DNA sequencing. The levels of triglycerides were found elevated and mean value was 2.45 ± 0.57 for patients with diabetic dyslipidemia as compared to diabetic and control groups. The levels of low-density lipoproteins were observed as normal with mean value of 1.50 ± 0.22.
Conclusion: The ABCA1 gene did not carry any genetic mutation in exon-9 in studied subjects. The raised level of triglyceride lipoproteins was a typical sign of dyslipidemia with a normal profile of low-density lipoproteins. |
| first_indexed | 2024-12-17T23:16:34Z |
| format | Article |
| id | doaj.art-7809176eb1994649b858867735f1d33f |
| institution | Directory Open Access Journal |
| issn | 0030-9648 2411-8842 |
| language | English |
| last_indexed | 2024-12-17T23:16:34Z |
| publishDate | 2020-10-01 |
| publisher | Army Medical College Rawalpindi |
| record_format | Article |
| series | Pakistan Armed Forces Medical Journal |
| spelling | doaj.art-7809176eb1994649b858867735f1d33f2022-12-21T21:29:00ZengArmy Medical College RawalpindiPakistan Armed Forces Medical Journal0030-96482411-88422020-10-0170512711276SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIAMunnaza Sarfraz0Amir Rashid1Midhat Mustafa2Saleem Ahmad Khan3Asifa Majeed4Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanObjective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia. Study Design: Cross-sectional comparative. Place and Duration of Study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi, from Jan to Dec 2017. Methodology: Ninety subjects were selected and divided into three groups, thirty in each. The division was based on newly diagnosed patients of type 2 diabetes with dyslipidemia, newly diagnosed patients of type 2 diabetes and healthy individuals. Genomic DNA was extracted from the blood samples of all subjects. Exon-9 of the ABCA1 gene was amplified through polymerase chain reaction and sequenced on automated DNA sequencer. The biochemical data was analyzed by SPSS-20 and presented in percentage and mean SD. Results: The pattern of sequences of ABCA1 gene was found normal in patients of the group I and II through DNA sequencing. The levels of triglycerides were found elevated and mean value was 2.45 ± 0.57 for patients with diabetic dyslipidemia as compared to diabetic and control groups. The levels of low-density lipoproteins were observed as normal with mean value of 1.50 ± 0.22. Conclusion: The ABCA1 gene did not carry any genetic mutation in exon-9 in studied subjects. The raised level of triglyceride lipoproteins was a typical sign of dyslipidemia with a normal profile of low-density lipoproteins.https://www.pafmj.org/index.php/PAFMJ/article/view/5548/2951abca1 genedna sequencingdyslipidemialow-density lipoproteinstriglycerides |
| spellingShingle | Munnaza Sarfraz Amir Rashid Midhat Mustafa Saleem Ahmad Khan Asifa Majeed SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA Pakistan Armed Forces Medical Journal abca1 gene dna sequencing dyslipidemia low-density lipoproteins triglycerides |
| title | SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA |
| title_full | SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA |
| title_fullStr | SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA |
| title_full_unstemmed | SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA |
| title_short | SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA |
| title_sort | screening of genetic mutation in abca1 gene and relationship of low density lipoprotein and triglycerides in patients with type 2 diabetes with and without dyslipidemia |
| topic | abca1 gene dna sequencing dyslipidemia low-density lipoproteins triglycerides |
| url | https://www.pafmj.org/index.php/PAFMJ/article/view/5548/2951 |
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