Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
<b>Background:</b> Measuring lipoprotein particle concentrations may help to improve cardiovascular risk stratification. Both the lipofit (Numares) and lipoprofile (LabCorp) NMR methods are widely used for the quantification of lipoprotein particle concentrations. <b>Objective:<...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2227-9059/10/7/1766 |
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| author | Martin Rief Reinhard Raggam Peter Rief Philipp Metnitz Tatjana Stojakovic Markus Reinthaler Marianne Brodmann Winfried März Hubert Scharnagl Günther Silbernagel |
| author_facet | Martin Rief Reinhard Raggam Peter Rief Philipp Metnitz Tatjana Stojakovic Markus Reinthaler Marianne Brodmann Winfried März Hubert Scharnagl Günther Silbernagel |
| author_sort | Martin Rief |
| collection | DOAJ |
| description | <b>Background:</b> Measuring lipoprotein particle concentrations may help to improve cardiovascular risk stratification. Both the lipofit (Numares) and lipoprofile (LabCorp) NMR methods are widely used for the quantification of lipoprotein particle concentrations. <b>Objective:</b> The aim of the present work was to perform a method comparison between the lipofit and lipoprofile NMR methods. In addition, there was the objective to compare lipofit and lipoprofile measurements of standard lipids with clinical chemistry-based results. <b>Methods:</b> Total, LDL, and HDL cholesterol and triglycerides were measured with ß-quantification in serum samples from 150 individuals. NMR measurements of standard lipids and lipoprotein particle concentrations were performed by Numares and LabCorp. <b>Results:</b> For both NMR methods, differences of mean concentrations compared to ß-quantification-derived measurements were ≤5.5% for all standard lipids. There was a strong correlation between ß-quantification- and NMR-derived measurements of total and LDL cholesterol and triglycerides (all r > 0.93). For both, the lipofit (r = 0.81) and lipoprofile (r = 0.84) methods, correlation coefficients were lower for HDL cholesterol. There was a reasonable correlation between LDL and HDL lipoprotein particle concentrations measured with both NMR methods (both r > 0.9). However, mean concentrations of major and subclass lipoprotein particle concentrations were not as strong. <b>Conclusions:</b> The present analysis suggests that reliable measurement of standard lipids is possible with these two NMR methods. Harmonization efforts would be needed for better comparability of particle concentration data. |
| first_indexed | 2024-03-09T03:39:29Z |
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| last_indexed | 2024-03-09T03:39:29Z |
| publishDate | 2022-07-01 |
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| series | Biomedicines |
| spelling | doaj.art-780aaba865fd4c5d8651e3f167fa0baf2023-12-03T14:43:04ZengMDPI AGBiomedicines2227-90592022-07-01107176610.3390/biomedicines10071766Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle ConcentrationsMartin Rief0Reinhard Raggam1Peter Rief2Philipp Metnitz3Tatjana Stojakovic4Markus Reinthaler5Marianne Brodmann6Winfried März7Hubert Scharnagl8Günther Silbernagel9Division of General Anaesthesiology, Emergency- and Intensive Care Medicine, Department of Anaesthesiology and Intensive Care Medicine, Medical University of Graz, A-8036 Graz, AustriaDivision of Angiology, Department of Internal Medicine, Medical University of Graz, A-8036 Graz, AustriaDivision of Angiology, Department of Internal Medicine, Medical University of Graz, A-8036 Graz, AustriaDivision of General Anaesthesiology, Emergency- and Intensive Care Medicine, Department of Anaesthesiology and Intensive Care Medicine, Medical University of Graz, A-8036 Graz, AustriaClinical Institute of Medical and Chemical Laboratory Diagnostics, University Hospital Graz, A-8036 Graz, AustriaDepartment of Cardiology (CBF), Charité-Universitätsmedizin Berlin, 12203 Berlin, GermanyDivision of Angiology, Department of Internal Medicine, Medical University of Graz, A-8036 Graz, AustriaClinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, A-8036 Graz, AustriaClinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, A-8036 Graz, AustriaDivision of Angiology, Department of Internal Medicine, Medical University of Graz, A-8036 Graz, Austria<b>Background:</b> Measuring lipoprotein particle concentrations may help to improve cardiovascular risk stratification. Both the lipofit (Numares) and lipoprofile (LabCorp) NMR methods are widely used for the quantification of lipoprotein particle concentrations. <b>Objective:</b> The aim of the present work was to perform a method comparison between the lipofit and lipoprofile NMR methods. In addition, there was the objective to compare lipofit and lipoprofile measurements of standard lipids with clinical chemistry-based results. <b>Methods:</b> Total, LDL, and HDL cholesterol and triglycerides were measured with ß-quantification in serum samples from 150 individuals. NMR measurements of standard lipids and lipoprotein particle concentrations were performed by Numares and LabCorp. <b>Results:</b> For both NMR methods, differences of mean concentrations compared to ß-quantification-derived measurements were ≤5.5% for all standard lipids. There was a strong correlation between ß-quantification- and NMR-derived measurements of total and LDL cholesterol and triglycerides (all r > 0.93). For both, the lipofit (r = 0.81) and lipoprofile (r = 0.84) methods, correlation coefficients were lower for HDL cholesterol. There was a reasonable correlation between LDL and HDL lipoprotein particle concentrations measured with both NMR methods (both r > 0.9). However, mean concentrations of major and subclass lipoprotein particle concentrations were not as strong. <b>Conclusions:</b> The present analysis suggests that reliable measurement of standard lipids is possible with these two NMR methods. Harmonization efforts would be needed for better comparability of particle concentration data.https://www.mdpi.com/2227-9059/10/7/1766nuclear magnetic resonance spectroscopyultracentrifugationlipoproteinslipoprotein subclassesmethodsanalysis |
| spellingShingle | Martin Rief Reinhard Raggam Peter Rief Philipp Metnitz Tatjana Stojakovic Markus Reinthaler Marianne Brodmann Winfried März Hubert Scharnagl Günther Silbernagel Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations Biomedicines nuclear magnetic resonance spectroscopy ultracentrifugation lipoproteins lipoprotein subclasses methods analysis |
| title | Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations |
| title_full | Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations |
| title_fullStr | Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations |
| title_full_unstemmed | Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations |
| title_short | Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations |
| title_sort | comparison of two nuclear magnetic resonance spectroscopy methods for the measurement of lipoprotein particle concentrations |
| topic | nuclear magnetic resonance spectroscopy ultracentrifugation lipoproteins lipoprotein subclasses methods analysis |
| url | https://www.mdpi.com/2227-9059/10/7/1766 |
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