Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD
<i>Panax vietnamensis</i> (PV), a wild <i>Panax</i> species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little informa...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/13/3086 |
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| author | Kim Long Vu-Huynh Huy Truong Nguyen Thi Hong Van Le Chi Thanh Ma Gwang Jin Lee Sung Won Kwon Jeong Hill Park Minh Duc Nguyen |
| author_facet | Kim Long Vu-Huynh Huy Truong Nguyen Thi Hong Van Le Chi Thanh Ma Gwang Jin Lee Sung Won Kwon Jeong Hill Park Minh Duc Nguyen |
| author_sort | Kim Long Vu-Huynh |
| collection | DOAJ |
| description | <i>Panax vietnamensis</i> (PV), a wild <i>Panax</i> species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins in PV during cultivation, but this information could serve as an indication of the appropriate harvest time. In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2–7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 were the major saponins that contributed to the change of PV’s saponin content through the years. In addition, the developed and validated HPLC method was proven to be reliable and effective for quality control of PV. |
| first_indexed | 2024-03-10T18:38:34Z |
| format | Article |
| id | doaj.art-780bd61c1ada445a8d881478742bbc02 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:38:34Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-780bd61c1ada445a8d881478742bbc022023-11-20T06:02:22ZengMDPI AGMolecules1420-30492020-07-012513308610.3390/molecules25133086Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSDKim Long Vu-Huynh0Huy Truong Nguyen1Thi Hong Van Le2Chi Thanh Ma3Gwang Jin Lee4Sung Won Kwon5Jeong Hill Park6Minh Duc Nguyen7Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, VietnamCollege of Pharmacy, Seoul National University, Seoul 151-742, KoreaCollege of Pharmacy, Seoul National University, Seoul 151-742, KoreaCollege of Pharmacy, Seoul National University, Seoul 151-742, KoreaFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam<i>Panax vietnamensis</i> (PV), a wild <i>Panax</i> species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins in PV during cultivation, but this information could serve as an indication of the appropriate harvest time. In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2–7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 were the major saponins that contributed to the change of PV’s saponin content through the years. In addition, the developed and validated HPLC method was proven to be reliable and effective for quality control of PV.https://www.mdpi.com/1420-3049/25/13/3086<i>Panax vietnamensis</i>vietnamese ginsengHPLC-UV/ELSDaccumulation of saponins |
| spellingShingle | Kim Long Vu-Huynh Huy Truong Nguyen Thi Hong Van Le Chi Thanh Ma Gwang Jin Lee Sung Won Kwon Jeong Hill Park Minh Duc Nguyen Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD Molecules <i>Panax vietnamensis</i> vietnamese ginseng HPLC-UV/ELSD accumulation of saponins |
| title | Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD |
| title_full | Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD |
| title_fullStr | Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD |
| title_full_unstemmed | Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD |
| title_short | Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD |
| title_sort | accumulation of saponins in underground parts of i panax vietnamensis i at different ages analyzed by hplc uv elsd |
| topic | <i>Panax vietnamensis</i> vietnamese ginseng HPLC-UV/ELSD accumulation of saponins |
| url | https://www.mdpi.com/1420-3049/25/13/3086 |
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