Hepatoprotective and Antifibrotic Effects of Indonesian Propolis
Liver cirrhosis is the irreversible stage in liver damage process which occurs after liver fibrosis due to necro-inflammatory activities and liver fibrosis. Therefore, inhibition of liver inflammation and fibrosis is very important to prevent liver cirrhosis. This study aimed to analyze the effect o...
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| Format: | Article |
| Language: | English |
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Universitas Padjajaran
2019-12-01
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| Series: | Majalah Kedokteran Bandung |
| Subjects: | |
| Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1768 |
| _version_ | 1819289483604918272 |
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| author | Diding Heri Prasetyo Sarsono Sarsono Ida Nurwati Prihandjojo Andri Putranto Martini Martini Nabila Aushaf Prasetyo |
| author_facet | Diding Heri Prasetyo Sarsono Sarsono Ida Nurwati Prihandjojo Andri Putranto Martini Martini Nabila Aushaf Prasetyo |
| author_sort | Diding Heri Prasetyo |
| collection | DOAJ |
| description | Liver cirrhosis is the irreversible stage in liver damage process which occurs after liver fibrosis due to necro-inflammatory activities and liver fibrosis. Therefore, inhibition of liver inflammation and fibrosis is very important to prevent liver cirrhosis. This study aimed to analyze the effect of ethanol extract of propolis (EEP) from mount Lawu, Indonesia to prevent liver damage and fibrosis progression in mice with hepatic cirrhosis. This study was performed during the period of June 2018 to May 2019 on a sample of 32 male Balb/C mice divided into control group (P1), induction of carbon tetrachloride (CCl4 ) group (P2), induction of 50 mg/BW CCl4 + EEP group (P3), and (induction of 100 mg/KgBW CCl4 + EEP (P4) with each group consisted of eight mice. The CCl4 in olive oil was administered intraperitoneally three times a week for six weeks. Mean differences between group was determined using ANOVA test with a significance level of 0.05. The induction of CCl4 increased liver cell damage and serum alanin aminotransferase (ALT) level. However, the addition of EEP significantly (p<0.001) reduced liver cell damage as seen in P3 (54.38±4.17 per 100 liver cells) and P4 (37.13±4.36 per 100 liver cells) groups and serum alanin aminotransferase (ALT) as seen in P3 (291.19±113.92 U/L) and P4 (229.38±73.45 U/L) groups. The APRI scores were also reduced after EEP as seen in P3 (0.738±0.292) and P4 (0.513±0.253) groups. Thus, EEP isolates from Gunung Lawu can reduce liver cell damage and fibrosis in mice model of hepatic cirrhosis. |
| first_indexed | 2024-12-24T03:07:34Z |
| format | Article |
| id | doaj.art-780fcb89f35c402d9e821e5f053fde48 |
| institution | Directory Open Access Journal |
| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-12-24T03:07:34Z |
| publishDate | 2019-12-01 |
| publisher | Universitas Padjajaran |
| record_format | Article |
| series | Majalah Kedokteran Bandung |
| spelling | doaj.art-780fcb89f35c402d9e821e5f053fde482022-12-21T17:17:56ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232019-12-0151313414010.15395/mkb.v51n3.17681134Hepatoprotective and Antifibrotic Effects of Indonesian PropolisDiding Heri Prasetyo0Sarsono Sarsono1Ida Nurwati2Prihandjojo Andri Putranto3Martini Martini4Nabila Aushaf Prasetyo5Department of Internal Medicine, School of Medicine, Sebelas Maret University/Dr. Moewardi Hospital, SurakartaBiochemistry Laboratory, Faculty of Medicine, Sebelas Maret University, SurakartaBiochemistry Laboratory, Faculty of Medicine, Sebelas Maret University, SurakartaBiochemistry Laboratory, Faculty of Medicine, Sebelas Maret University, SurakartaBiochemistry Laboratory, Faculty of Medicine, Sebelas Maret University, SurakartaFaculty of Medicine, Sebelas Maret University, SurakartaLiver cirrhosis is the irreversible stage in liver damage process which occurs after liver fibrosis due to necro-inflammatory activities and liver fibrosis. Therefore, inhibition of liver inflammation and fibrosis is very important to prevent liver cirrhosis. This study aimed to analyze the effect of ethanol extract of propolis (EEP) from mount Lawu, Indonesia to prevent liver damage and fibrosis progression in mice with hepatic cirrhosis. This study was performed during the period of June 2018 to May 2019 on a sample of 32 male Balb/C mice divided into control group (P1), induction of carbon tetrachloride (CCl4 ) group (P2), induction of 50 mg/BW CCl4 + EEP group (P3), and (induction of 100 mg/KgBW CCl4 + EEP (P4) with each group consisted of eight mice. The CCl4 in olive oil was administered intraperitoneally three times a week for six weeks. Mean differences between group was determined using ANOVA test with a significance level of 0.05. The induction of CCl4 increased liver cell damage and serum alanin aminotransferase (ALT) level. However, the addition of EEP significantly (p<0.001) reduced liver cell damage as seen in P3 (54.38±4.17 per 100 liver cells) and P4 (37.13±4.36 per 100 liver cells) groups and serum alanin aminotransferase (ALT) as seen in P3 (291.19±113.92 U/L) and P4 (229.38±73.45 U/L) groups. The APRI scores were also reduced after EEP as seen in P3 (0.738±0.292) and P4 (0.513±0.253) groups. Thus, EEP isolates from Gunung Lawu can reduce liver cell damage and fibrosis in mice model of hepatic cirrhosis.http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1768altapri scoreliver cirrhosisliver damagepropolis |
| spellingShingle | Diding Heri Prasetyo Sarsono Sarsono Ida Nurwati Prihandjojo Andri Putranto Martini Martini Nabila Aushaf Prasetyo Hepatoprotective and Antifibrotic Effects of Indonesian Propolis Majalah Kedokteran Bandung alt apri score liver cirrhosis liver damage propolis |
| title | Hepatoprotective and Antifibrotic Effects of Indonesian Propolis |
| title_full | Hepatoprotective and Antifibrotic Effects of Indonesian Propolis |
| title_fullStr | Hepatoprotective and Antifibrotic Effects of Indonesian Propolis |
| title_full_unstemmed | Hepatoprotective and Antifibrotic Effects of Indonesian Propolis |
| title_short | Hepatoprotective and Antifibrotic Effects of Indonesian Propolis |
| title_sort | hepatoprotective and antifibrotic effects of indonesian propolis |
| topic | alt apri score liver cirrhosis liver damage propolis |
| url | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1768 |
| work_keys_str_mv | AT didingheriprasetyo hepatoprotectiveandantifibroticeffectsofindonesianpropolis AT sarsonosarsono hepatoprotectiveandantifibroticeffectsofindonesianpropolis AT idanurwati hepatoprotectiveandantifibroticeffectsofindonesianpropolis AT prihandjojoandriputranto hepatoprotectiveandantifibroticeffectsofindonesianpropolis AT martinimartini hepatoprotectiveandantifibroticeffectsofindonesianpropolis AT nabilaaushafprasetyo hepatoprotectiveandantifibroticeffectsofindonesianpropolis |