Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite
The rapid and sensitive detection of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is essential to ensure food safety and protect humans from foodborne diseases. In this study, a sensitive and facile electrochemical immunosensor using AuPdPt trimetallic nanoparticles functi...
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MDPI AG
2021-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/4/446 |
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| author | En Han Yun Zhang Jianrong Cai Xinai Zhang |
| author_facet | En Han Yun Zhang Jianrong Cai Xinai Zhang |
| author_sort | En Han |
| collection | DOAJ |
| description | The rapid and sensitive detection of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is essential to ensure food safety and protect humans from foodborne diseases. In this study, a sensitive and facile electrochemical immunosensor using AuPdPt trimetallic nanoparticles functionalized multi-walled carbon nanotubes (MWCNTs-AuPdPt) as the signal amplification platform was designed for the label-free detection of <i>S. aureus</i>. The nanocomposite of MWCNTs-AuPdPt was prepared by an in situ growth method of loading AuPdPt trimetallic nanoparticles on the surface of MWCNTs. The synthesized MWCNTs-AuPdPt featured good conductivity and superior catalytic performance for hydrogen peroxide. The nanocomposite of MWCNTs-AuPdPt with good biocompatibility and high specific surface area was further functionalized by anti-<i>S. aureus</i> antibodies. The immobilized antibodies could efficiently capture <i>S. aureus</i> to the modified electrode by an immune reaction, which resulted in the change of catalytic current intensity to realize the sensitive detection of <i>S. aureus</i>. The designed immunosensor could detect <i>S. aureus</i> in a linear range from 1.1 × 10<sup>2</sup> to 1.1 × 10<sup>7</sup> CFU mL<sup>−1</sup> with a low detection limit of 39 CFU mL<sup>−1</sup>. Additionally, the proposed immunosensor was successfully applied to determine <i>S. aureus</i> in actual samples with acceptable results. This strategy provided a promising platform for highly sensitive determination of <i>S. aureus</i> and other pathogens in food products. |
| first_indexed | 2024-03-10T12:16:31Z |
| format | Article |
| id | doaj.art-78148cfc2d9045398200fb438c170b1d |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T12:16:31Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-78148cfc2d9045398200fb438c170b1d2023-11-21T15:48:24ZengMDPI AGMicromachines2072-666X2021-04-0112444610.3390/mi12040446Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized NanocompositeEn Han0Yun Zhang1Jianrong Cai2Xinai Zhang3School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaThe rapid and sensitive detection of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is essential to ensure food safety and protect humans from foodborne diseases. In this study, a sensitive and facile electrochemical immunosensor using AuPdPt trimetallic nanoparticles functionalized multi-walled carbon nanotubes (MWCNTs-AuPdPt) as the signal amplification platform was designed for the label-free detection of <i>S. aureus</i>. The nanocomposite of MWCNTs-AuPdPt was prepared by an in situ growth method of loading AuPdPt trimetallic nanoparticles on the surface of MWCNTs. The synthesized MWCNTs-AuPdPt featured good conductivity and superior catalytic performance for hydrogen peroxide. The nanocomposite of MWCNTs-AuPdPt with good biocompatibility and high specific surface area was further functionalized by anti-<i>S. aureus</i> antibodies. The immobilized antibodies could efficiently capture <i>S. aureus</i> to the modified electrode by an immune reaction, which resulted in the change of catalytic current intensity to realize the sensitive detection of <i>S. aureus</i>. The designed immunosensor could detect <i>S. aureus</i> in a linear range from 1.1 × 10<sup>2</sup> to 1.1 × 10<sup>7</sup> CFU mL<sup>−1</sup> with a low detection limit of 39 CFU mL<sup>−1</sup>. Additionally, the proposed immunosensor was successfully applied to determine <i>S. aureus</i> in actual samples with acceptable results. This strategy provided a promising platform for highly sensitive determination of <i>S. aureus</i> and other pathogens in food products.https://www.mdpi.com/2072-666X/12/4/446trimetallic nanoparticleselectrochemical immunosensorlabel-free detection<i>Staphylococcus aureus</i> |
| spellingShingle | En Han Yun Zhang Jianrong Cai Xinai Zhang Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite Micromachines trimetallic nanoparticles electrochemical immunosensor label-free detection <i>Staphylococcus aureus</i> |
| title | Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite |
| title_full | Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite |
| title_fullStr | Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite |
| title_full_unstemmed | Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite |
| title_short | Development of Highly Sensitive Immunosensor for Detection of <i>Staphylococcus aureus</i> Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite |
| title_sort | development of highly sensitive immunosensor for detection of i staphylococcus aureus i based on aupdpt trimetallic nanoparticles functionalized nanocomposite |
| topic | trimetallic nanoparticles electrochemical immunosensor label-free detection <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2072-666X/12/4/446 |
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