The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots
The effect of inhibitors of plasma membrane oxidoreductases (quinacrine and dicumarol) and H+-ATPase (dicyclohexylcarbodiimide and orthovanadate) on ammonium uptake by Pisum arvense seedlings and the activities of H+-ATPase and NADH-ferricyanide oxidoreductase was investigated. The uptake solution c...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1113 |
| _version_ | 1818548381652353024 |
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| author | Genowefa Kubik-Dobosz Aleksandra Turska Halina Lekacz Waldemar Karcz Józef Buczek |
| author_facet | Genowefa Kubik-Dobosz Aleksandra Turska Halina Lekacz Waldemar Karcz Józef Buczek |
| author_sort | Genowefa Kubik-Dobosz |
| collection | DOAJ |
| description | The effect of inhibitors of plasma membrane oxidoreductases (quinacrine and dicumarol) and H+-ATPase (dicyclohexylcarbodiimide and orthovanadate) on ammonium uptake by Pisum arvense seedlings and the activities of H+-ATPase and NADH-ferricyanide oxidoreductase was investigated. The uptake solution contained 50 µM NH4+. In I h experiments, quinacrine and dicumarol depressed strongly and irreversibly the rate of NH4+ uptake and markedly inhibited the activity of NADH-ferri-cyanide oxidoreductase in the plasma membrane vesicles prepared from root cells. Simultaneously, sodium orthovanadate inhibited the activity of plasma membrane H+-ATPase increased the rate of NH4+ uptake. Dicyclohexylcarbodiimide inhibited H+-ATPase activity and increased efflux of NH4+ from roots to ambient solution. The results indicate on the lack of direct connection between uptake rate of 50 µM NH4+ and H+-ATPase activity, and suggest that membrane redox systems play a predominant role in this process. |
| first_indexed | 2024-12-12T08:19:10Z |
| format | Article |
| id | doaj.art-78150abd0a3b4a7a917085297a00a334 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T08:19:10Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-78150abd0a3b4a7a917085297a00a3342022-12-22T00:31:27ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01633-428729210.5586/asbp.1994.039849The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense rootsGenowefa Kubik-Dobosz0Aleksandra Turska1Halina Lekacz2Waldemar Karcz3Józef Buczek4Wrocław UniversityWrocław UniversityUniversity of SilesiaUniversity of SilesiaWrocław UniversityThe effect of inhibitors of plasma membrane oxidoreductases (quinacrine and dicumarol) and H+-ATPase (dicyclohexylcarbodiimide and orthovanadate) on ammonium uptake by Pisum arvense seedlings and the activities of H+-ATPase and NADH-ferricyanide oxidoreductase was investigated. The uptake solution contained 50 µM NH4+. In I h experiments, quinacrine and dicumarol depressed strongly and irreversibly the rate of NH4+ uptake and markedly inhibited the activity of NADH-ferri-cyanide oxidoreductase in the plasma membrane vesicles prepared from root cells. Simultaneously, sodium orthovanadate inhibited the activity of plasma membrane H+-ATPase increased the rate of NH4+ uptake. Dicyclohexylcarbodiimide inhibited H+-ATPase activity and increased efflux of NH4+ from roots to ambient solution. The results indicate on the lack of direct connection between uptake rate of 50 µM NH4+ and H+-ATPase activity, and suggest that membrane redox systems play a predominant role in this process.https://pbsociety.org.pl/journals/index.php/asbp/article/view/1113ammoniumuptakeinhibitorsoxidoreductase NADH-ferricyanideH+ - ATPase |
| spellingShingle | Genowefa Kubik-Dobosz Aleksandra Turska Halina Lekacz Waldemar Karcz Józef Buczek The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots Acta Societatis Botanicorum Poloniae ammonium uptake inhibitors oxidoreductase NADH-ferricyanide H+ - ATPase |
| title | The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots |
| title_full | The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots |
| title_fullStr | The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots |
| title_full_unstemmed | The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots |
| title_short | The effect of inhibitors of plasma membrane H+ - ATPase and oxidoreductases on NH4+ uptake by Pisum arvense roots |
| title_sort | effect of inhibitors of plasma membrane h atpase and oxidoreductases on nh4 uptake by pisum arvense roots |
| topic | ammonium uptake inhibitors oxidoreductase NADH-ferricyanide H+ - ATPase |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1113 |
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